A5004372889- Hemoglobinopathies and Related Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Hemoglobin structure and function
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
- Iron Metabolism and Disorders
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Neonatal Health and Biochemistry
- Barrier Structure and Function Studies
- Mycobacterium research and diagnosis
- Blood groups and transfusion
- Genomics and Rare Diseases
- Pancreatitis Pathology and Treatment
- MRI in cancer diagnosis
- Hepatocellular Carcinoma Treatment and Prognosis
- Heme Oxygenase-1 and Carbon Monoxide
- Medical Imaging Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Neurological Disease Mechanisms and Treatments
- Liver Disease Diagnosis and Treatment
- Neuroscience of respiration and sleep
Nagoya University
2014-2025
Hyogo Prefectural Cancer Center
2024
Yamaguchi University
1991-2024
Yokohama City University Hospital
2021
Yokohama City University
2021
Japan Society for the Promotion of Science
2018-2020
Kanazawa Medical Center
2020
Kyoto University
2011-2015
Washington Center
2015
Nagoya City University
2013
In the central nervous system, endothelial cells (ECs) and pericytes (PCs) of blood vessel walls cooperatively form a physical chemical barrier to maintain neural homeostasis. However, in diabetic retinopathy (DR), loss PCs from is assumed cause breakdown blood-retina (BRB) subsequent vision-threatening vascular dysfunctions. Nonetheless, lack adequate DR animal models has precluded disease understanding drug discovery. Here, by using an anti-PDGFRβ antibody, we show that transient...
The relationships between tissue-resident microglia and early macrophages, especially their lineage segregation outside the yolk sac, have been recently explored, providing a model in which conversion from macrophages seeds during brain development. However, spatiotemporal evidence to support such microglial seeding situ explain how it occurs has not obtained. By cell tracking via slice culture, intravital imaging, Flash tag-mediated or genetic labeling, we find that intraventricular CD206+...
An array of lipidic compounds that constitute the cell wall mycobacteria is recognized by host receptors. Examples include trehalose dimycolate (TDM), which a major surface-exposed glycolipid mycobacteria, interacts with macrophage inducible C-type lectin, Mincle, and exerts its highly potent adjuvant functions. Recent evidence has suggested glycerol monomycolate (GroMM), another mycolate-containing lipid species produced can stimulate innate immune cells; however, specific receptors have...
Iron overload of a chronic nature has been associated with wide variety human diseases, including infection, carcinogenesis, and atherosclerosis. Recently, highly specific turn-on fluorescent probe (RhoNox-1) to labile ferrous iron [Fe(II)], but not ferric [Fe(III)], was developed. The evaluation Fe(II) is more important than Fe(III) in vivo that an initiating component the Fenton reaction. In this study, we applied frozen sections established reaction-based rat renal carcinogenesis model...
In the developing cortex, postmigratory neurons accumulate in cortical plate (CP) to properly differentiate consolidating subtype identities. Microglia, despite their extensive surveying activity, temporarily disappear from midembryonic CP. However, mechanism and significance of this absence are unknown. Here, we show that microglia bidirectionally migrate via attraction by CXCL12 released meninges subventricular zone thereby exit Upon nonphysiological excessive exposure vivo or vitro, young...
Three monoclonal antibodies, 1-7 (gamma 2b), 3-5 1), and 8-30 (mu), specific to Fc epsilon receptors (Fc R) on human B cells were established. The two monoclonals (1-7 8-30) could inhibit the binding of IgE R in rosette formation assays, as well FACS analysis, shown recognize same epitope R. other antibody (3-5) recognized molecule but a different epitope, marginally inhibited binding. molecules RPMI 8866 by these antibodies had Mr 46,000 25,000 30,000 daltons determined immunoprecipitation...
While amyloid-β lies upstream of tau pathology in Alzheimer’s disease, key drivers for other tauopathies, including progressive supranuclear palsy (PSP), are largely unknown. Various mutations known to facilitate aggregation, but how the nonmutated tau, which most cases with PSP share, increases its propensity aggregate neurons and glial cells has remained elusive. Here, we identified genetic variations protein abundance filamin-A brains without mutations. We provided vivo biochemical...
To evaluate retrospectively the correlation between enhancement patterns on dynamic computed tomography (CT) and angiogenesis fibrosis in pancreatic adenocarcinoma.Twenty-three patients with adenocarcinoma underwent CT tumor resection. In addition to absolute relative enhanced value that was calculated by subtracting attenuation pre-contrast from those contrast-enhanced each phase, we defined one parameter, "tumor-aorta ratio", which dividing of cancer abdominal aorta phase. These were...
Proteinase-activated receptor 2 (PAR(2)) is a G-protein coupled associated with many pathophysiological functions. To date, the development of PAR(2) antagonists has been limited. Here, we identify number novel peptide-mimetic and demonstrate inhibitory effects on PAR(2)-mediated intracellular signalling pathways vascular responses.The compound library based structures agonist peptides were screened for inhibition PAR(2)-induced calcium mobilisation in human keratinocytes. Representative...
Neocortical development proceeds through the formation of new zones in which neural-lineage cells are organized based on their differentiation status. Although microglia initially distribute homogeneously throughout growing cerebral wall, they accumulate inner cytogenic zone, ventricular zone (VZ) and subventricular (SVZ) mid-embryonic stage. However, roles these remain to be elucidated. In this study, we found that microglia, despite being only a minor population constitute promote neural...
Delayed-type hypersensitivity (DTH) is marked by high levels of protein antigen-specific T cell responses in sensitized individuals. Recent evidence has revealed a distinct pathway for immunity directed against glycolipid antigens, but DTH to this class antigen been undetermined and difficult prove due their insolubility aqueous solutions. Here, glucose monomycolate (GMM), highly hydrophobic the wall mycobacteria, was dispersed solutions form octaarginine-modified liposomes tested its...
Human CD1b molecules contain a maze of hydrophobic pockets and tunnel capable accommodating the unusually long, branched acyl chain mycolic acids, an essential fatty acid component cell wall mycobacteria. It has been accepted that CD1b-bound acids constitute scaffold for mycolate-containing (glyco)lipids stimulating CD1b-restricted T cells. Remarkable homology in amino sequence is observed between human monkey molecules, indeed, are able to bind glucose monomycolate (GMM), glucosylated...