A5042004829- Radiation Therapy and Dosimetry
- Nuclear Physics and Applications
- Radiation Detection and Scintillator Technologies
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Nicotinic Acetylcholine Receptors Study
- Nuclear physics research studies
- Enzyme Structure and Function
- Advanced Radiotherapy Techniques
- Matrix Theory and Algorithms
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Particle accelerators and beam dynamics
- Plant biochemistry and biosynthesis
- Advanced NMR Techniques and Applications
- Ion-surface interactions and analysis
- Plant Gene Expression Analysis
- Bacterial Genetics and Biotechnology
- Ion channel regulation and function
- Advanced Optimization Algorithms Research
- Fusion and Plasma Physics Studies
- RNA Research and Splicing
- Medical Imaging Techniques and Applications
- Particle Accelerators and Free-Electron Lasers
- X-ray Spectroscopy and Fluorescence Analysis
Kitasato University
2012-2024
Okayama University of Science
1995-2023
Tokyo Institute of Technology
2008-2019
Japan Radioisotope Association
2019
Mitsubishi Group (Japan)
2003-2015
Motion Control (United States)
2013
Institute of Life Sciences
1996-2011
Kumamoto University
2011
Gunma University
1998-2009
Ibaraki University
2009
Families bearing mutations in the presenilin 1 (PS1) gene develop Alzheimer’s disease. Previous studies have shown that Alzheimer-associated PS1 increase production of amyloid β protein (Aβ 1–42 ). We now show also regulates phosphorylation microtubule-associated tau. directly binds tau and a kinase, glycogen synthase kinase 3β (GSK-3β). Deletion both GSK-3β bind to same region PS1, residues 250–298, whereas binding domain on is microtubule-binding repeat region. The ability bring into close...
The production of projectile fragments is one the most important, but not yet perfectly understood, problems to be considered when planning for utilization high-energy heavy charged particles radiotherapy. This paper reports our investigation fragments' fluence and linear energy transfer (LET) spectra produced from various incident ions using an experimental approach reveal these physical qualities beams. Polymethyl methacrylate, as a substitute human body, was used target. A ΔE–E counter...
Monochromatic x-ray computed tomography (CT) at two different energies provides information about electron density of human tissue without ambiguity due to the beam hardening effect. This makes treatment planning for proton and heavy-ion radiotherapy more precise. We have started a feasibility study on dual energy CT by using synchrotron radiation. A translation–rotation scanning system was developed quantitative measurement in order clarify what precision achieved. Liquid samples solutions...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-Dimensional Structure in Solution of the Calcium Channel Blocker .omega.-Conotoxin MVIIAToshiyuki Kohno, Jae Il Kim, Kuniko Kobayashi, Yoshio Kodera, Tadakazu Maeda, and Kazuki SatoCite this: Biochemistry 1995, 34, 32, 10256–10265Publication Date (Print):August 15, 1995Publication History Published online1 May 2002Published inissue 15 August 1995https://pubs.acs.org/doi/10.1021/bi00032a020https://doi.org/10.1021/bi00032a020research-articleACS...
SGTx1 is a peptide toxin isolated from the venom of spider Scodra griseipes that has been shown to inhibit outward K+ currents in rat cerebellar granule neurons. Although its amino acid sequence known be highly (76%) homologous with hanatoxin (HaTx), well-characterized modifier Kv2.1 channel gating, structural and functional characteristics remain largely unknown. Here we describe NMR solution structure SGTx1, mechanism interaction channels, effect on activity once bound. The contains...
Our cell survival data showed the obvious dependence of RBE on photon energy: The value for 200 kV X-rays was approximately 10% greater than those mega-voltage beams. In radiation therapy using beams, energy distribution outside field is different with that in because a large number low scattering photons. Hence, values become greater. To evaluate increase RBE, method deriving Microdosimetric Kinetic model (MK model) proposed this study. MK has two kinds parameters, tissue-specific...
HsPKS1 from Huperzia serrata is a type III polyketide synthase (PKS) with remarkable substrate tolerance and catalytic potential. Here we present the synthesis of unnatural unique polyketide–alkaloid hybrid molecules by exploiting enzyme reaction using precursor-directed structure-based approaches. produced novel pyridoisoindole (or benzopyridoisoindole) 6.5.6-fused 6.6.5.6-fused) ring system condensation 2-carbamoylbenzoyl-CoA 3-carbamoyl-2-naphthoyl-CoA), synthetic nitrogen-containing...
The three-dimensional structure of amyloid β peptide (25−35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total 20 converged structures were obtained on the basis 110 experimental constraints, including 106 distance constraints reduced from nuclear Overhauser effect (NOE) connectivities and four torsion angle (φ) constraints. atomic root mean square difference about averaged...
The latest heavy ion therapy tends to require information about the spatial distribution of quality radiation in a patient's body order make best use any potential advantage swift ions for therapeutic treatment tumour. deflection incident particles is described well by Molière's multiple-scattering theory primary particles; however, projectile fragments not yet thoroughly understood. This paper reports on our investigation produced from carbon beam through nuclear reactions thick water. A...
A series of analogs omega-conotoxin GVIA, a peptide neurotoxin having 27 amino acid residues with three disulfide bridges, were synthesized by replacing each residue except for Cys and Hyp Ala. CD spectra virtually identical between native all the analogs, indicating overall conformations not changed substitutions. The inhibitory effects these on 125I-omega-conotoxin GVIA binding to chick brain synaptic plasma membranes showed that replacement Tyr13 Ala drastically lowered affinity toxin...
The three-dimensional structure of a complex cinnamycin, 19-amino acid residue immunopotentiator peptide, and lysophosphatidylethanolamine was determined by 1H-NMR. cylindrical in shape, 11 Å diameter 26 length, excluding the acyl chain phospholipid. peptide had hydrophobic pocket surrounded . residues Phe-7 through Ala(S)-14 to bind head group ligand. Fitting so good that other than glycerophosphoethanol-amine would be unable fit pocket. goodness fitting is compatible with strict...
The action mechanism of sapecin, an antibacterial peptide with membrane permeabilization activity, was investigated. dose dependence the caused by sapecin sigmoidal, suggesting that oligomerization leads to permeabilization. Solution nuclear magnetic resonance analysis sapecin-phospholipid vesicle complex revealed surface buried in and on molecule. membrane-buried determined observing transferred cross-saturation phenomena from alkyl chains phospholipid amide protons sapecin. contains basic...
Ficolins are a kind of pathogen-recognition molecule in the innate immune systems. To investigate discrimination mechanism between self and non-self by ficolins, we determined crystal structure human M-ficolin fibrinogen-like domain (FD1), which is ligand-binding domain, at 1.9A resolution. Although FD1 monomer shares common fold with fibrinogen gamma fragment tachylectin-5A, Asp-282-Cys-283 peptide bond, predicted site on C-terminal P normal trans unlike cases other two proteins. The...
A cDNA encoding a novel plant type III polyketide synthase was cloned and sequenced from the Chinese club moss Huperzia serrata (Huperziaceae). The deduced amino acid sequence of Hu. 1 showed 44–66% identity to those other chalcone superfamily enzymes origin. Further, phylogenetic tree analysis revealed that groups with nonchalcone‐producing synthases. Indeed, recombinant enzyme expressed in Escherichia coli unusually versatile catalytic potential produce various aromatic tetraketides,...