A5032258234- Blood disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Neutropenia and Cancer Infections
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Systemic Sclerosis and Related Diseases
- Autoimmune and Inflammatory Disorders Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Caveolin-1 and cellular processes
- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Mast cells and histamine
- interferon and immune responses
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory Myopathies and Dermatomyositis
- Sphingolipid Metabolism and Signaling
Research Center Borstel - Leibniz Lung Center
2002-2025
German Center for Lung Research
2020-2025
Medizinische Hochschule Hannover
1995-2000
Familial hemophagocytic lymphohistiocytosis (FHL) is a rare autosomal recessive disorder characterized by hyperactive phagocytes and defects in natural killer cell function. It has been shown previously that mutations the perforin 1 gene (PRF1) UNC13D are associated with FHL2 FHL3, respectively, indicating genetic heterogeneity. We performed genome-wide homozygosity mapping large consanguineous Kurdish kindred five children affected FHL. Linkage to 10 cM region on chromosome 6q24 between...
Abstract Platelet factor 4 (PF-4), a platelet-derived CXC chemokine, has been shown to induce the differentiation of monocytes into subset macrophages that lack expression HLA-DR Ag. This suggests potential role for PF-4 in modulation monocyte-dependent T cell activation. Using an Ag-specific stimulation model which cells were cocultured with presence recall Ags, we could show under these conditions PF-4-treatment caused strong decrease proliferation as well IFN-γ release. However,...
Abstract Platelet factor 4 (PF-4), a platelet-derived CXC chemokine, is known to prevent human monocytes from apoptosis and promote differentiation of these cells into HLA-DR− macrophages. In this study, we investigated the role PF-4 in control acute monocyte proinflammatory responses involved direct combat microbial invaders. We show that increases phagocytosis provokes strong formation oxygen radicals but lacks chemotactic activity cells. Compared with FMLP, PF-4-induced oxidative burst...
Syntaxin 11 (STX11) controls vesicular trafficking and is a key player in exocytosis. Since Stx11 mutations are causally associated with familial hemophagocytic lymphohistio-cytosis, we wanted to clarify whether STX11 functionally important for immune cell populations. This was studied primary cells obtained from newly generated Stx11(-/-) mice. Our data revealed that not only widely expressed different cells, but also induced upon LPS or IFN-γ treatment. However, deficiency does affect...
To determine contributions and functions of autoantibodies (Abs) directed to the angiotensin receptor type 1 (AT1R), which are suggested be involved in pathogenesis AT1R Abs-related diseases such as systemic sclerosis (SSc).
Background In addition to serving as the primary effector cells against infections, neutrophils have been implicated in regulation of both innate and adaptive immunity. this study, we aimed investigate role immune system under physiological conditions. Methods The vivo effect on was examined using neutropenic mice. interaction between γδ T investigated an vitro co-culture system. Findings Unexpectedly, observed accumulation cervical lymph nodes Transcriptomic analysis revealed that these...
Abstract Platelet factor 4 (PF4; CXCL4) is an abundant platelet α-granule CXC chemokine with unique functions. Although lacking a chemotactic activity, PF4 initiates signal transduction cascade in human monocytes leading to the induction of broad spectrum acute and delayed functions including phagocytosis, respiratory burst, survival, secretion cytokines. Surprisingly, although these monocyte are well defined, only very limited information exists on specific signaling pathways that involved...
Objective The contribution of sustained autologous autoantibody production by B cells to the pathogenesis systemic sclerosis (SSc) and granulomatosis with polyangiitis (GPA) is not fully understood. To investigate this, a humanized mouse model was generated transferring patient-derived peripheral blood mononuclear (PBMC) into immunocompromised mice. Methods PBMC derived from patients SSc GPA as well healthy controls (HD) were isolated, characterized flow cytometry, infused Rag2 -/- /IL2rg In...
Human monocytes respond to a variety of stimuli with complex spectrum activities ranging from acute defense mechanisms cell differentiation or cytokine release. However, the individual intracellular signaling pathways related these functions are not well understood. CXC chemokine ligand 4 (CXCL4) represents broad activator monocytes, which induces as delayed in cells including differentiation, survival, release ROS, and cytokines. Here, we report for first time that CXCL4-treated...
SARS-CoV-2 enters into human airway epithelial cells via membrane fusion or endocytosis, and this process is dependent on ACE2, TMPRSS2, cathepsin L. In study, we examined the expression profiles of three entry genes in primary isolated from smokers, non-smokers, patients with chronic obstructive pulmonary disease lung cancer. An exhaustive search GEO database was performed to identify eligible data 1st June 2020. total, 46 datasets comprising transcriptomic 3,053 samples were identified as...
Point mutations in the gene for G-CSF receptor have been reported previously a subgroup of patients with severe congenital neutropenia. Here, we investigated frequency these specific neutropenic disorders undergoing treatment recombinant human (r-metHu)G-CSF (Filgrastim). Nucleotides 2306 to 2561, including critical region (nucleotides 2384-2429) from intracellular domain gene, were amplified by reverse transcriptase-polymerase chain reaction, and DNA was sequenced directly after...
Recently, a novel humanized mouse model for systemic sclerosis (SSc) was established by transferring peripheral blood mononuclear cells (PBMC) from patients with SSc to Rag2
Abstract A modified technique of two‐dimensional gel electrophoresis (2‐DE) was used to investigate differences in the pattern cytosolic proteins neutrophilic granulocytes from patients with severe congenital neutropenia, cyclic and idiopathic neutropenia comparison healthy donors. At time study, all tested received treatment a recombinant human granulocyte colony‐stimulating factor (r‐metHuG‐CSF; Filgrastim, Amgen). Using Investigator 2‐D Electrophoresis System™ (Millipore) we were able...
In-vitro models of differentiated primary human airway epithelial cells are a valuable tool to study severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Through the use these models, it has been shown that expression SARS-CoV-2 entry genes in epithelia is influenced by various factors such as age, sex, smoking status, and pathogenic conditions. In this study, we aimed determine effects cell culture conditions donor demographic clinical characteristics on including...
Abstract SARS-CoV-2 enters into human airway epithelial cells via membrane fusion or endocytosis, and this process is dependent on ACE2, TMPRSS2, cathepsin L. In study, we examined the expression profiles of three entry-related genes in primary isolated from donors with different physiological pathological backgrounds such as smoking, COPD, asthma, lung cancer, allergic rhinitis, cystic fibrosis, viral infections. By reanalyzing 54 GEO datasets comprising transcriptomic data 3428 samples,...