A5031511174- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Mitochondrial Function and Pathology
- Isotope Analysis in Ecology
University of Münster
2021-2025
Institute of Medical Microbiology and Hygiene
2024
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) makes it possible to simultaneously visualize the spatial distribution of dozens hundreds different biomolecules (e.g., phospho- and glycolipids) in tissue sections cell cultures. The implementation novel desorption (post-)ionization techniques has recently pushed pixel size this technique low micrometer scale below thus a cellular potentially sub-cellular level. However, fully exploit potential for biology...
Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing software solutions MALDI MSI data analysis are incomplete, require programming skills contain laborious manual steps, hindering broadly applicable, reproducible, high-throughput generate impactful biological...
The precise fatty acyl chain configuration of cardiolipin (CL), a tetrameric mitochondrial-specific membrane lipid, exhibits dependence on cell and tissue types. A powerful method to map CL profiles in sections spatially resolved manner is matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). To build explore this potential, we employed quadrupole time-of-flight spectrometer along with optimized sample preparation protocols. We imaged the individual murine...
Abstract Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry (MALDI MSI) and immunofluorescence microscopy holds great potential for understanding pathological mechanisms mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing open-source software solutions analysis of MALDI MSI data are incomplete, require programming skills contain laborious manual steps, hindering broadly applicable, reproducible,...
We introduce Fluorescence Integrated Single-Cell Analysis Script (FISCAS), which combines fluorescence microscopy with MALDI-MSI to streamline single-cell analysis. FISCAS enables automated selection of tight measurement regions, thereby reducing the acquisition off-target pixels, and makes use established algorithms for cell segmentation coregistration rapidly compile spectra. MALDI-compatible staining membranes, nuclei, lipid droplets allows collection data prior on a timsTOF fleX MALDI-2....
The rapidly evolving field of spatial biology revolves around the analysis cells in their native microenvironment. This can include morphological features, presence specific antigens or gene expression. To add another layer information, recent methodological advances matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) now enable untargeted lipids and metabolites at subcellular resolution. integration MALDI-MSI single-cell level with established optical...