A5004797408- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Ion-surface interactions and analysis
- Analytical chemistry methods development
- Glycosylation and Glycoproteins Research
- Advanced Chemical Sensor Technologies
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Bacterial Identification and Susceptibility Testing
- Erythrocyte Function and Pathophysiology
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Legume Nitrogen Fixing Symbiosis
- Viral gastroenteritis research and epidemiology
- Studies on Chitinases and Chitosanases
- MRI in cancer diagnosis
- Sphingolipid Metabolism and Signaling
- Mechanical Failure Analysis and Simulation
- Electric and Hybrid Vehicle Technologies
- Data Visualization and Analytics
- Real-time simulation and control systems
- Species Distribution and Climate Change
- Marine Biology and Ecology Research
University of Münster
2016-2025
Institute of Medical Microbiology and Hygiene
2024
Netherlands Metabolomics Centre
2023
Leiden University Medical Center
2023
Center for Clinical Research (United States)
2017
Institute for Atomic and Molecular Physics
2013-2014
University Hospital Münster
2009
Imaging lipid composition Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main components, such as abundant phosphatidylcholine species. Soltwisch et al. used tunable for post-ionization neutral species boost other membrane cholesterol and phospho- glycolipids. cells tissues these methods allows differentiation based on more extensive chemical...
Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing software solutions MALDI MSI data analysis are incomplete, require programming skills contain laborious manual steps, hindering broadly applicable, reproducible, high-throughput generate impactful biological...
Abstract Matrix‐assisted laser desorption ionization mass spectrometry imaging (MALDI‐MSI) visualizes the distribution of phospho‐ and glycolipids in tissue sections. However, C=C double‐bond (db) positional isomers generally cannot be distinguished. Now an on‐tissue Paternò–Büchi (PB) derivatization procedure that exploits benzaldehyde as a MALDI‐MSI‐compatible reagent is introduced. Laser‐induced postionization (MALDI‐2) was used to boost yields protonated PB products. Collision‐induced...
Matrix-assisted laser desorption/ionization combined with laser-induced postionization (MALDI-2) is a recently introduced method for enhanced mass spectrometry imaging of numerous classes biomolecules, including phospho- and glycolipids in tissue sections at high lateral resolution. Here we describe the first adaptation technology to Bruker timsTOF fleX spectrometer. Upon use 1 kHz laser, MALDI-2 produces sizable increase number detected features as well ion signal intensities. This...
Coupling laser post-ionisation with a high resolving power MALDI Orbitrap mass spectrometer has realised an up to ∼100-fold increase in the sensitivity and enhanced chemical coverage for MALDI-MS imaging of lipids relative conventional MALDI. This could constitute major breakthrough biomedical research.
N-glycans are important players in a variety of pathologies including different types cancer, (auto)immune diseases, and also viral infections. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an tool for high-throughput N-glycan profiling and, upon use tandem MS, structure determination. By MALDI-MS imaging (MSI) combination with PNGase F treatment, spatially correlated from tissue sections becomes possible. Here we coupled laser-induced postionization, or...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) makes it possible to simultaneously visualize the spatial distribution of dozens hundreds different biomolecules (e.g., phospho- and glycolipids) in tissue sections cell cultures. The implementation novel desorption (post-)ionization techniques has recently pushed pixel size this technique low micrometer scale below thus a cellular potentially sub-cellular level. However, fully exploit potential for biology...
Molecular analysis on the single-cell level represents a rapidly growing field in life sciences. While bulk from pool of cells provides general molecular profile, it is blind to heterogeneities between individual cells. This heterogeneity, however, an inherent property every cell population. Its fundamental understanding development, function, and role specific same genotype that display different phenotypical properties. Single-cell mass spectrometry (MS) aims provide broad information for...
The laser wavelength constitutes a key parameter in ultraviolet-matrix-assisted desorption ionization-mass spectrometry (UV-MALDI-MS). Optimal analytical results are only achieved at wavelengths that correspond to high optical absorption of the matrix. In presented work, dependence and contribution matrix proton affinity MALDI process were investigated. A tunable dye was used examine range between 280 355 nm. peptide ion signals recorded as function these irradiation parameters displayed...
Mass spectrometers from the Synapt-G1/G2 family (Waters) are widely employed for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). A lateral resolution of about 50 μm is typically achieved with these instruments, that is, however, below often desired cellular resolution. Here, we show first MALDI-MSI examples demonstrating a ten micrometers obtained Synapt G2-S HDMS spectrometer without oversampling. This improvement became possible by beam shaping using 4:1...
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is widely used to visualize and analyze the distribution of membrane lipids in an increasingly large number applications. In this context, different lipoforms glycerophospholipids (GPLs) are among prime targets interest. For group analytes, however, ion suppression effects have been described strongly favor detection certain GPL classes over others, thereby hampering analysis suppressed species greatly...
Arsenic-containing lipids (arsenolipids) are natural products of marine organisms such as fish, invertebrates, and algae, many which important seafoods. A major group arsenolipids, namely, the arsenic-containing hydrocarbons (AsHC), have recently been shown to be cytotoxic human liver bladder cells, a result that has stimulated interest in chemistry toxicology these compounds. In this study, elemental laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) molecular...
Visualizing the differential distribution of carbon-carbon double bond (C═C db) positional isomers unsaturated phospholipids (PL) in tissue sections by use refined matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) technologies offers a high promise to deeper understand PL metabolism and isomer-specific functions health disease. Here we introduce an on-tissue ozonization protocol that enables particular straightforward derivatization lipids sections....
The main cellular receptors of Shiga toxins (Stxs), the neutral glycosphingolipids (GSLs), globotriaosylceramide (Gb3Cer/CD77) and globotetraosylceramide (Gb4Cer), are significantly upregulated in about half human colorectal carcinomas (CRC) other cancers. Therefore, conjugates exploiting Gb3Cer/Gb4Cer-binding B subunit Stx (StxB) have attracted great interest for both diagnostic adjuvant therapeutic interventions. Moreover, fucosylated GSLs were recognized as potential tumor-associated...
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. However, for many analyte classes, its sensitivity limited due to poor ionization efficiencies. To mitigate this problem, we here introduce novel post-ionization scheme based on single-photon induced chemical using pulsed RF-Kr lamps. The fine-vacuum conditions of dual ion-funnel ion source effectively thermalize evolving MALDI plume and enable ample gas-phase...
Although water ice has been utilized in the past as a matrix for infrared matrix-assisted laser desorption/ionization mass spectrometry (IR-MALDI-MS), it not found wider use due to limitations analytical performance and technical demands on employment of necessary cooling stage. Here, we developed temperature-controlled sample stage with an orthogonal time-of-flight spectrometer (MALDI-o-TOF-MS). The utilizes combination liquid nitrogen counterheating Peltier element. It allows adjustment...
To improve the lateral resolution in matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) beyond dimensions of focal spot oversampling techniques are employed. However, few data available on effect size and its beam profile ion signals recorded mode. investigate these dependencies, we produced 2 times six spots with between ~30 200 μm. By optional use a fundamental shaper, square flat-top Gaussian profiles were compared. MALDI-MSI collected using fixed pixel 20...
A recently introduced technique based on MALDI with laser-induced postionization (PI), also named MALDI-2, increases the ion yields for numerous classes of lipids, metabolites, and carbohydrates in MALDI-MS imaging experiments under certain experimental conditions. Here, we used a semiautomatic LabVIEW-based protocol to investigate optimize efficiency PI process dependent four relevant input parameters dense parameter grid: pulse energies two lasers, delay between laser pulses, buffer gas...
MALDI mass spectrometry imaging (MALDI-MSI) is a widely used technique to map the spatial distribution of molecules in sectioned tissue. The based on systematic generation and analysis ions from small sample volumes, each representing single pixel investigated surface. Subsequently, spectrometric images for any recorded ion species can be generated by displaying signal intensity at coordinate origin these pixels. Although easily equalized, intensities, however, are not necessarily good...
Abstract Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is widely used for the analysis of large biomolecules in numerous applications. The technique utilizes nanosecond-long pulses at various spot sizes to eject and ionize molecules embedded a highly absorptive chemical matrix. Despite methods name, ‘molecular desorption’ from matrix crystal surface not sole mechanism discussed material ejection MALDI, but additional ablation larger clusters has been reported. Here...
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is increasingly used to visualize the chemical communication between microorganisms. However, fully exploit potential of this label-free technique, crucial methodological advances are still needed. In particular, with current microbial MALDI-MSI methods coverage strongly limited well ionizing compounds and a safe MSI-compatible inactivation viability quenching metabolism not possible. Here, we introduce...