A5039510400- Legionella and Acanthamoeba research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacillus and Francisella bacterial research
- Bacterial biofilms and quorum sensing
- Advanced Proteomics Techniques and Applications
- Vibrio bacteria research studies
- Urinary Tract Infections Management
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Listeria monocytogenes in Food Safety
- Poxvirus research and outbreaks
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Pediatric Urology and Nephrology Studies
- Cancer Research and Treatments
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Immune cells in cancer
- Dye analysis and toxicity
- Diverse Scientific Research in Ukraine
Essen University Hospital
2023-2025
University of Duisburg-Essen
2023-2025
National Academy of Agrarian Sciences of Ukraine
2022
University of Rijeka
2017
Helmholtz Centre for Infection Research
2013-2017
Technische Universität Braunschweig
2009-2017
University of Würzburg
2009-2014
Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing software solutions MALDI MSI data analysis are incomplete, require programming skills contain laborious manual steps, hindering broadly applicable, reproducible, high-throughput generate impactful biological...
ABSTRACT Histological and clinical investigations describe late stages of Legionnaires' disease but cannot characterize early events human infection. Cellular or rodent infection models lack the complexity tissue have nonhuman backgrounds. Therefore, we developed applied a novel model for Legionella pneumophila comprising living lung tissue. We stimulated explants with L. strains outer membrane vesicles (OMVs) to analyze damage, bacterial replication, localization as well transcriptional...
ABSTRACT Antimicrobial resistance (AMR) has emerged as a significant global healthcare problem. Antibiotic use accelerated the physiologic process of AMR, particularly in Gram-negative pathogens. Urinary tract infections (UTIs) are predominantly nature. Uropathogens evolutionarily highly adapted and selected strains with specific virulence factors, suggesting common mechanisms how bacterial cells acquire AMR factors. The simultaneous increase is complex context-dependent phenomenon. Among...
REVIEW article Front. Microbiol., 25 April 2011Sec. Cellular and Infection Microbiology - closed section volume 2 2011 | https://doi.org/10.3389/fmicb.2011.00074
Abstract Infection with enterohemorrhagic E. coli (EHEC) causes severe changes in the brain leading to angiopathy, encephalopathy and microglial activation. In this study, we investigated role of tumour necrosis factor alpha (TNF-α) for activation pathology using a preclinical mouse model EHEC infection. LC–MS/MS proteomics mice injected combination Shiga toxin (Stx) lipopolysaccharide (LPS) revealed extensive alterations proteome, particular enrichment pathways involved complement...
<title>Abstract</title> Despite advancements in cancer therapies, bacterial complications remain a major challenge, delaying treatment and worsening outcomes. While immunosuppressive therapies prolonged hospitalizations contribute, they do not fully explain the elevated infection risk patients. We observed that G-CSF production by tumors was associated with persistence of Gram-negative pathogens head neck squamous cell carcinoma The presence oral rinse positively correlated poor prognosis...
Legionnaires' disease is an acute fibrinopurulent pneumonia. During infection Legionella pneumophila adheres to the alveolar lining and replicates intracellularly within recruited macrophages. Here we provide a sequence domain composition analysis of L. PilY1 protein, which has high homology Pseudomonas aeruginosa. proteins both pathogens contain von Willebrand factor A (vWFa) C-terminal PilY domain. Using cellular fractionation, assigned as outer membrane protein that only expressed during...
Protein glycosylation is an essential post-translational modification in all domains of life. Its impairment humans can result severe diseases named congenital disorders (CDGs). Most the glycosyltransferases (GTs) responsible for proper are polytopic membrane proteins that represent challenging targets proteomics. We established a multiple reaction monitoring (MRM) assay to comprehensively quantify GTs involved processes
Introduction Streptococcus pneumoniae is one of the main causes community-acquired infections in lung alveoli children and elderly. Alveolar macrophages (AM) patrol homeostasis under infectious conditions. However, molecular adaptations AM upon with are incompletely resolved. Methods We used a comparative transcriptomic proteomic approach to provide novel insights into cellular mechanism that changes signature during infections. Using tandem mass spectrometry murine cell-sorted AM, we...
Thyroid hormone (TH) transporter MCT8 deficiency causes severe locomotor disabilities likely due to insufficient TH transport across brain barriers and, consequently, compromised neural action. As an established animal model for this disease, Mct8/Oatp1c1 double knockout (DKO) mice exhibit strong central deprivation, impairments and similar histo-morphological features as seen in patients. The pathways that cause these neuro-motor symptoms are poorly understood. In paper, we performed...
Hepes-glutamic acid buffer-mediated organic solvent protection effect (HOPE)-fixation has been introduced as an alternative to formalin fixation of clinical samples. Beyond preservation morphological structures for histology, HOPE-fixation was demonstrated be compatible with recent methods RNA and DNA sequencing. However, the suitability HOPE-fixed materials inspection proteomes by mass spectrometry so far remained undefined. This is particular interest, since proteins constitute a prime...
Immune evasion of cancer is induced by interaction between immune cells with tumor microenvironment (TME). CCL2 a major chemoattractant for myeloid in TME and associated immunosuppression at the site poor prognosis. Aiming to prevent immunosuppressive TME, we developed peptide fragment CCL2, MMIb, which blocks glycosaminoglycan-CCL2 thus disturbs guiding chemokine gradient cells. In translational approach, effect MMIb was evaluated vitro vivo murine model oropharyngeal carcinoma, verified...
Urinary tract infections (UTIs) are common affecting the urinary system, predominantly caused by bacterial pathogens, with Escherichia coli being most frequent pathogen. Infections of kidney (eg, pyelonephritis) severe and challenging to treat, due specific tissue microenvironment. In this study, influence different parameters mimicking environment on effectiveness antibiotics prescribed for pyelonephritis growth uropathogenic strains was analyzed.
Abstract Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry (MALDI MSI) and immunofluorescence microscopy holds great potential for understanding pathological mechanisms mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing open-source software solutions analysis of MALDI MSI data are incomplete, require programming skills contain laborious manual steps, hindering broadly applicable, reproducible,...
is a highly infectious gram-negative bacterium that causes tularemia in humans and animals. It can survive multiply variety of cells, including macrophages, dendritic amoebae, arthropod-derived cells. However, the intracellular life cycle varies depending on cell type. Shortly after infection mammalian escapes phagosome into cytosol, where it replicates. In contrast, amoebae
Abstract Motivation: InFiRe, Insertion Finder via Restriction digest, is a novel software tool that allows for the computational identification of transposon insertion sites in known bacterial genome sequences after mutagenesis experiments. The approach based on fact restriction endonuclease digestions DNA yield unique pattern fragments with defined sizes. Transposon changes size hosting fragment by number base pairs. exact this can be determined Southern blot hybridization. Subsequently,...
Francisella is a gram-negative bacterial pathogen, which causes tularemia in humans and animals. A crucial step of infection its invasion macrophage cells. Biogenesis the Francisella-containing phagosome (FCP) arrested for ~15 minutes at endosomal stage, followed by gradual escape into cytosol, where microbe proliferates. The pathogenesis short transient presence bacterium within phagosome. Isolation FCPs further studies has been challenging due to period time residence it characteristics...
Strawberry (Fragaria ananassa Duch.) plants are valuable for the high taste, nutritional, medicinal and dietary properties of their fruits. Strawberries first to open season fresh berries. They especially rich in sugars, organic acids, pectin, vitamins mineral elements. The total area strawberry plantations Ukraine, according State Statistics Service, is 8200 ha. average yield reaches 6.9 tons/ha. In order develop an effective disease protection system, it necessary detect diagnose time. An...