A5045251824- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Drug-Induced Hepatotoxicity and Protection
- Galectins and Cancer Biology
- Advanced Proteomics Techniques and Applications
- Autophagy in Disease and Therapy
- Vitamin C and Antioxidants Research
- Heat shock proteins research
- Immunodeficiency and Autoimmune Disorders
- Genomics and Phylogenetic Studies
- Bacterial Infections and Vaccines
- Blood disorders and treatments
- Bacterial Genetics and Biotechnology
- Retinal Development and Disorders
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
- COVID-19 Clinical Research Studies
- Erythrocyte Function and Pathophysiology
- Streptococcal Infections and Treatments
- Complement system in diseases
Heidelberg University
2020-2025
DKFZ-ZMBH Alliance
2020-2025
Leibniz Institute for Analytical Sciences - ISAS
2020-2024
Max Planck Institute for Medical Research
2018
Abstract Background Presently, antibody concentration measurements for patients undergoing treatment are predominantly determined by ELISA, which still comes with known disadvantages. Therefore, our aim was to establish a targeted mass-spectrometric assay enabling the reproducible absolute quantification of peptides from hypervariable and interaction regions infliximab. Methods Peptides infliximab were measured post-trypsin digestion subsequent separation on Vanquish Horizon UHPLC coupled...
ABSTRACT Lysosomes are membrane-enclosed organelles that play a crucial role in the degradation of intra- and extracellular substrates regulation metabolic signaling. These functions carried out by variety proteins, which > 150 currently known to be located lysosomal lumen or embedded its membrane. proteins typically low abundant, necessitating organelle enrichment experiments enable their investigation unbiased mass spectrometry-based proteomics analyses. Enrichment strategies have been...
As novel liquid chromatography–mass spectrometry (LC-MS) technologies for proteomics offer a substantial increase in LC-MS runs per day, robust and reproducible sample preparation emerges as new bottleneck throughput. We introduce strategy positive-pressure 96-well filter-aided (PF96) on commercial solid-phase extraction device. PF96 allows five-fold throughput conjunction with extraordinary reproducibility Pearson product-moment correlations the protein level of r = 0.9993, demonstrated...
Protein glycosylation is an essential post-translational modification in all domains of life. Its impairment humans can result severe diseases named congenital disorders (CDGs). Most the glycosyltransferases (GTs) responsible for proper are polytopic membrane proteins that represent challenging targets proteomics. We established a multiple reaction monitoring (MRM) assay to comprehensively quantify GTs involved processes
Abstract Background The SARS-CoV-2/COVID-19 pandemic has inflicted medical and socioeconomic havoc, despite the current availability of vaccines broad implementation vaccination programs, more easily accessible cost-effective acute treatment options preventing morbidity mortality are urgently needed. Herbal teas have historically recurrently been applied as self-medication for prophylaxis, therapy, symptom alleviation in diverse diseases, including those caused by respiratory viruses,...
Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology activation of toxins induces stasis or programmed cell death by interference with vital cellular processes. Zeta toxins, which prevalent in many genomes, were shown to interfere wall formation perturbing peptidoglycan synthesis Gram-positive bacteria. Here, we characterize the epsilon/zeta (TA) homologue from Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1. Contrary previously...
•TSR1 of ADAMTS16 can be C-mannosylated.•Deletion DPY19L1 or DPY19L3 in hiPSCs caused reduced secretion ADAMTS16.•Targeting dpy19l3 medaka occasionally led to coloboma. C-mannosylation is a modification tryptophan residues with single mannose and affect protein folding, secretion, and/or function. To date, only few proteins have been demonstrated C-mannosylated, studies that globally assess are scarce. interrogate the C-mannosylome human induced pluripotent stem cells, we compared secretomes...
Abstract The current SARS-CoV-2/COVID-19 pandemic wreaks medical and socioeconomic havoc. Despite the availability of vaccines, cost-effective acute treatment options preventing morbidity mortality are urgently needed. To identify affordable, ubiquitously available, effective treatments, we tested herbs consumed worldwide as herbal teas regarding their antiviral activity against SARS-CoV-2. Aqueous infusions prepared by boiling leaves Lamiaceae perilla sage elicit potent sustained SARS-CoV-2...
Abstract The collapse of protein homeostasis manifests itself in a toxic aggregation cascade, which is associated with degenerative diseases and aging. To solubilize aggregates, dedicated disaggregases exist unicellular organisms, but these have no nuclear/cytosolic orthologs metazoa. Alternative metazoan disaggregation machines been described, how are operated regulated vivo remained unknown. We show that functionally diversified human cells to efficiently target different types...
ABSTRACT Defects in the evolutionarily conserved protein-glycosylation machinery during embryonic development are often fatal. Consequently, congenital disorders of glycosylation (CDG) human rare. We modelled a putative hypomorphic mutation described an alpha-1,3/1,6-mannosyltransferase (ALG2) index patient (ALG2-CDG) to address developmental consequences teleost medaka (Oryzias latipes). observed specific, multisystemic, late-onset phenotypes, closely resembling patient's syndrome,...
Abstract Protein glycosylation is essential in all domains of life and its mutational impairment humans can result severe diseases named Congenital Disorders Glycosylation (CDGs). Studies on molecular level are however challenging, because many glycosyltransferases the endoplasmic reticulum (ER) low abundance membrane proteins. We established a comprehensive multiple reaction monitoring (MRM) assay to quantify most human involved processes N -glycosylation, O - C -mannosylation ER. To...
Abstract Patients suffering from Congenital Disorders of Glycosylation (CDG) carry mutations in components the evolutionarily highly conserved protein-glycosylation-machinery. Employing targeted genome editing, we modeled alleles medaka fish based on a mutation described an ALG2-index patient. The multisystemic phenotypes our alg2 model closely resembled patient’s syndromes. Molecularly, results reduction abundance N -glycans without altering profile glycan structures as well patient...