A5017526751- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Advanced Neural Network Applications
- Machine Learning and Data Classification
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Advanced Proteomics Techniques and Applications
- Galectins and Cancer Biology
- Calcium signaling and nucleotide metabolism
- Radiomics and Machine Learning in Medical Imaging
- Electrochemical sensors and biosensors
- Urinary and Genital Oncology Studies
- Phagocytosis and Immune Regulation
- Cardiac Ischemia and Reperfusion
- Advanced Photocatalysis Techniques
- Medical Image Segmentation Techniques
- Ferroptosis and cancer prognosis
- Bladder and Urothelial Cancer Treatments
- Virus-based gene therapy research
- Advanced Nanomaterials in Catalysis
- Cardiac Arrest and Resuscitation
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Mechanical Circulatory Support Devices
University of Freiburg
2020-2024
University Medical Center Freiburg
2023-2024
Heidelberg University
2019-2020
University of Cambridge
2019
Pancreatic ductal adenocarcinoma (PDAC) represents one of the most aggressive and lethal malignancies worldwide with an urgent need for new diagnostic therapeutic strategies. One major risk factor PDAC is pre-indication chronic pancreatitis (CP), which highly inflammatory pancreatic tissue. Kallikreins (KLKs) are secreted serine proteases that play important role in various cancers as components tumor microenvironment. Previous studies KLKs solid tumors largely relied on either...
Protein glycosylation is an essential post-translational modification in all domains of life. Its impairment humans can result severe diseases named congenital disorders (CDGs). Most the glycosyltransferases (GTs) responsible for proper are polytopic membrane proteins that represent challenging targets proteomics. We established a multiple reaction monitoring (MRM) assay to comprehensively quantify GTs involved processes
Abstract State‐of‐the‐art mass spectrometers combined with modern bioinformatics algorithms for peptide‐to‐spectrum matching (PSM) robust statistical scoring allow more variable features (i.e., post‐translational modifications) being reliably identified from (tandem‐) spectrometry data, often without the need biochemical enrichment. Semi‐specific proteome searches, that enforce a theoretical enzymatic digestion to solely N‐ or C‐terminal end, identify of native protein termini those arising...
The complexity of the transcriptome is governed by intricate interplay transcription, RNA processing, translocation, and decay. In eukaryotes, removal 5’-RNA cap essential for initiation degradation. addition to canonical 5’-N7-methyl guanosine in ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) was identified as a new structure prokaryotic eukaryotic organisms. So far, two classes NAD-RNA decapping enzymes have been identified, namely Nudix that liberate mononucleotide...
Clonal isolation is an integral step of numerous workflows in genome editing and cell engineering. It comprises the a single progenitor from defined line population with subsequent expansion to obtain monoclonal population. This process associated transient loss cell-cell contacts absence multicellular microenvironment. Previous studies have revealed transcriptomic changes upon clonal specific extent. Since transcriptome alterations are only partially reflected on proteome level, we sought...
State-of-the-art mass spectrometers combined with modern bioinformatics algorithms for peptide-to-spectrum matching (PSM) robust statistical scoring allow more variable features (i.e., post-translational modifications) being reliably identified from (tandem-) spectrometry data, often without the need biochemical enrichment. Semi-specific proteome searches, that enforces a theoretical enzymatic digestion to solely N- or C-terminal end, identify native protein termini those arising endogenous...
Only a small number of patients survive an out-of-hospital cardiac arrest (CA) and can be discharged from hospital alive with large percentage these retaining neurological impairments. In recent years, extracorporeal cardiopulmonary resuscitation (ECPR) has emerged as beneficial strategy to optimize treatment. However, ECPR is still associated various complications. To reduce problems, profound understanding the underlying mechanisms required. This study aims investigate effects CA,...
Hybrid flavin–polydopamine nanoparticles combine the activity of an enzyme cofactor with stabilising and electron-transfering properties nanostructured poly-dopamine to afford a green biocompatible catalytic system tuneable by visible light.
Abstract We present the proteomic profiling of 79 bladder cancers, including treatment‐naïve non‐muscle‐invasive cancer (NMIBC, n = 17), muscle‐invasive (MIBC, 51), and neoadjuvant‐treated MIBC ( 11). Proteins were extracted from formalin‐fixed, paraffin‐embedded samples analyzed using data‐independent acquisition, yielding >8,000 quantified proteins. MIBC, compared to NMIBC, shows an extracellular matrix (ECM) immune response signature as well alteration metabolic proteome together with...
Abstract Protein glycosylation is essential in all domains of life and its mutational impairment humans can result severe diseases named Congenital Disorders Glycosylation (CDGs). Studies on molecular level are however challenging, because many glycosyltransferases the endoplasmic reticulum (ER) low abundance membrane proteins. We established a comprehensive multiple reaction monitoring (MRM) assay to quantify most human involved processes N -glycosylation, O - C -mannosylation ER. To...
Abstract Pancreatic ductal adenocarcinoma (PDAC) represents one of the most aggressive and lethal malignancies worldwide with an urgent need for new diagnostic therapeutic strategies. One major risk factor PDAC is pre-indication chronic pancreatitis (CP), which highly inflammatory pancreatic tissue. Kallikreins (KLKs) are secreted serine proteases that play important role in various cancers as components tumor microenvironment. Previous studies KLKs solid tumors largely relied on either...