A5061847307- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Liver physiology and pathology
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Cardiac, Anesthesia and Surgical Outcomes
- Biomedical Ethics and Regulation
- Pancreatic function and diabetes
- Medical History and Innovations
- Tissue Engineering and Regenerative Medicine
- Genetics and Neurodevelopmental Disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Mesenchymal stem cell research
- Neuroscience of respiration and sleep
- Ocular Surface and Contact Lens
- Biochemical and Molecular Research
- Connexins and lens biology
- Cancer-related gene regulation
- Microfluidic and Bio-sensing Technologies
- Corneal surgery and disorders
- Airway Management and Intubation Techniques
- Sphingolipid Metabolism and Signaling
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
Glasgow Caledonian University
2018
University of Oslo
2013-2017
MRC Centre for Regenerative Medicine
2010-2015
University of Edinburgh
2010-2015
Roslin Institute
2013
Medical Research Council
2010-2012
Keele University
2010
In recent years, the use of a simple inkjet technology for cell printing has triggered tremendous interest and established field biofabrication. A key challenge been development processes which are both controllable less harmful, in order to preserve tissue viability functions. Here, we report on valve-based printer that validated print highly viable cells programmable patterns from two different bio-inks with independent control volume each droplet (with lower limit 2 nL or fewer than five...
The differentiation of pluripotent stem cells to hepatocytes is well established, yet current methods suffer from several drawbacks. These include a lack definition and reproducibility, which in part stems continued reliance on recombinant growth factors. This has remained stumbling block for the translation technology into industry clinic reasons associated with cost quality. We have devised growth-factor-free protocol that relies small molecules differentiate human toward hepatic...
In the epidermis, remodelling of Connexin43 is a key event in wound closure. However, controversy between role connexin channel and non-channel functions exist. We compared impact SiRNA targeted to mimetic peptide Gap27 on scrape closure rates hemichannel signalling adult keratinocytes (AK) fibroblasts sourced from juvenile foreskin (JFF), human neonatal (HNDF) dermal tissue (ADF). The these agents, following 24 h exposure, GJA1 (encoding Connexin43), Ki67 TGF-β1 gene expression, pSmad3...
Abstract A challenge facing the human pluripotent stem cell (hPSC) field is variability observed in differentiation potential of hPSCs. Variability can lead to time consuming and costly optimisation yield type interest. This especially relevant for hPSCs towards endodermal lineages. Endodermal cells have promising new knowledge therapies diseases affecting multiple organ systems, including lung, thymus, intestine, pancreas liver, as well applications regenerative medicine toxicology....
Human embryonic stem cells (hESCs) offer an inexhaustible supply of human somatic cell types through their ability to self-renew while retaining pluripotency. As such, hESC-derived are important for applications ranging from in vitro modeling therapeutic use. However, full potential be realized, both the growth undifferentiated and derivatives must performed defined culture conditions. Many research groups maintain hESCs using mouse fibroblasts (MEF) MEF conditioned medium (CM). The use...
The coordination of signalling pathways within the cell is vital for normal human development and post-natal tissue homeostasis. Gene expression function therefore tightly controlled at a number levels. We investigated role that post-translational modifications play during hepatocyte differentiation. In particular, we examined small ubiquitin-like modifier (SUMO) proteins in this process. used embryonic stem (hESC)-based model differentiation to follow changes protein SUMOylation. Moreover,...
It has been suggested that the isolation of scalable populations limbal stem cells may lead to radical changes in ocular therapy. In particular, derivation and transplantation corneal from these result therapies providing clinical normality diseased or damaged cornea. Although feasible theory, lack donor material sufficient quantity quality currently limits such a strategy. A potential source could be derived pluripotent (PSCs). We developed an vitro serum-free differentiation model which...
Adult hepatocytes are polarised with their apical and basolateral membranes separated from neighbouring cells by tight junction proteins. Although efficient differentiation of pluripotent stem to has been achieved, the formation proper polarisation in these not thoroughly investigated. In present study, human embryonic (hESCs) mesenchymal (hMSCs) were differentiated hepatocyte-like derived characterised for mature hepatocyte markers. The secretion hepatic proteins, expression genes...
We have devised an embryoid body–based screening method for the selection of human embryonic stem cell (hESC) lines capable forming functional hepatocyte-like cells (HLCs) after single-cell dissociation. The highlighted one line from a panel five that produced albumin-positive during body (EB) formation. Cell did not produce EB formation were shown to respond less well directed differentiation following replating. Additionally, seeding density pluripotent populations prior was exert...
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