A5102016446- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Pulmonary Hypertension Research and Treatments
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Cardiovascular Function and Risk Factors
- Immune Cell Function and Interaction
- Cardiac Valve Diseases and Treatments
- Lysosomal Storage Disorders Research
- Autoimmune Bullous Skin Diseases
- PARP inhibition in cancer therapy
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Vascular Malformations and Hemangiomas
- Spondyloarthritis Studies and Treatments
- Prostate Cancer Treatment and Research
- Prenatal Screening and Diagnostics
- Proteoglycans and glycosaminoglycans research
- Trypanosoma species research and implications
- Rheumatoid Arthritis Research and Therapies
- Eosinophilic Disorders and Syndromes
- Glycogen Storage Diseases and Myoclonus
- Immune Response and Inflammation
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2025
Agostino Gemelli University Polyclinic
2021-2025
University of the Sacred Heart
2024
Università Cattolica del Sacro Cuore
2024
Hospital Universitario de Fuenlabrada
2020
Menéndez Pelayo International University
1998-2003
Comunidad de Madrid
2001-2003
Universidad de Jaén
2003
Centro de Investigaciones Biológicas Margarita Salas
2003
Centro de Biología Molecular Severo Ochoa
2002
The main objective of the present study was to determine role CD34 + cell subsets in haemopoietic recovery children undergoing peripheral blood stem transplantation. For this purpose, 38 leukaphereses from 33 with malignancies mobilized G‐CSF were analysed. Using dual‐colour flow cytometry, different subpopulations cells quantified and number each reinfused correlated resurgence. Multivariate analysis showed that CD38 − better time neutrophil platelet recovery, respectively, than total...
Abstract Background The COVID-19 pandemic has posed a huge challenge to healthcare systems and their personnel worldwide. study of the impact SARS-CoV-2 infection among workers, through prevalence studies, will let us know viral expansion, individuals at most risk exposed areas. aim this is gauge in our hospital workforce identify groups areas increased risk. Methods Findings This cross-sectional longitudinal carried out on workers based molecular serological diagnosis infection. Of 3013 HCW...
Abstract: We report the complete coding sequence of a new HLA‐B27 subtype, B*2712, which was found in Caucasian Spanish family within chromosome A2‐Cw2‐B*2712‐DR15‐DQ6. B*2712 first detected as segregating B blank Bw6‐associated antigen. Extensive serologic analysis demonstrated that this B27 subtype not recognised by any B27‐monospecific antibodies, giving positive reactions only with some monoclonal reagents against B40 or B27,40. Sequencing showed high similarity B*2708, differing three...
The development of molecular techniques for HLA typing has allowed the identification genes previously assigned as serologic blank alleles. Lack or poor cell surface expression been found molecules coded by HLA-A, -B, -DRB4, -DRB5, and -DPB1 genes. In this report we describe first HLA-C gene encoding a null molecule. HLA-Cw*0409 N shows point deletion at position 1095 within exon 7. This mutation provokes codon reading shift, generating new translation stop 97 bp downstream to that described...
On the basis of susceptibility normal myelomonocytic cells to adenoviral vectors, we have studied possibility selectively transducing murine leukemic (WEHI-3B) with regular (Reg-Ad) and genetically modified (RGD-Ad) vectors. An 8-h incubation WEHI-3B 100 pfu Reg-Ad vectors/cell resulted in whole population becoming positive for transgene expression. Under identical conditions infection, 20–30% mouse bone marrow (BM) were transgene. When RGD-Ad vectors used, a brief exposure (10 min) 150...
The aim of this study was to characterize the lymphocyte and hematopoietic stem progenitor cell (HPC) subsets cryopreserved premature cord blood (PCB) compared term (TCB) by flow cytometry, influence birth conditions, assess its availability for transplantation.Four-color cytometric analysis performed on 43 PCB 40 TCB samples using a panel 24 different mAbs, directed against lymphoid HPC surface markers. CB volume estimated weight newborn determine absolute MNC CD34(+) content/CB sample....
Abstract: HLA‐B18 is a well defined Bw6‐associated serologic specificity. Up to now, four different sequences have been characterised in Caucasian populations (B*1801,3,4,5), and one Orientals (B*1802). We report new subtype (B*1806) which was serologically detected Spanish individual as B18 Bw4‐associated antigen. Complete coding region sequencing showed that B*1806 differs from B*1801 unique nu‐cleotide at position 299 (A T), giving rise an amino acid replacement residue 76 (glutamic...
Abstract: HLA‐J345, in contrast to B44, does not show molecular polymorphism. We have found a group of Caucasian Spanish individuals, sero‐logically typed as B45, showing an unexpected HLA‐B12 PCR‐SSO subtyp‐ing pattern. Complete coding region sequencing and B45 subtyping by demonstrated that the j345 serologic specificity is constituted two alleles: B*4501 B*5002. B*5002 recognized polyclonal monoclonal allosera against B12 whereas it detected B21, B49, or B50 reagents, providing new...
Abstract: Four new HLA classical class I alleles in the three loci are described Caucasian individuals. A*3012 was first suspected by an abnormal serologic pattern that would be explained single amino acid substitution at A30‐specific Ser17. B*270505 differs from B*270502 a silent up to now constant position B locus. B*3541 encodes for Cys 118 has not been encountered neither human nor primate alleles. Cw*0716 seems originated large‐scale interallelic recombination event between...
Abstract: Two novel human leukocyte antigen (HLA) class II alleles for DRB3 and DQB1 genes detected in Caucasoid Spanish individuals are described: DRB3*0218 DQB1*030202. Both have been found during routine high‐resolution typing by sequencing. shows a gene polymorphic position, located at amino acid residue 58, alanine to glutamic acid. This is shared several DRB1 alleles, including all described DRB1*11 subtypes. DQB1*030202 differs from DQB1*030201 point mutation position 319 (T C)....
Abstract Background and purpose Takotsubo syndrome (TTS) may lead to serious in-hospital complications. Our study aims investigate the prognostic impact of right ventricular-to-pulmonary artery (RV-PA) coupling in patients with TTS. Methods Consecutive TTS were prospectively enrolled. RV function was evaluated by global longitudinal strain (RVGLS) free wall (RVFWS) RV-PA measured as ratio either tricuspid annular plane systolic excursion (TAPSE), RVGLS or RVFWS pulmonary pressure (PASP)....
The broad HLA‐B16 serologic specificity is divided into B38 and B39 splits associated to Bw4 Bw6, respectively. Differential variants have been defined for several molecular subtypes of B38. We found a Spanish Caucasian individual carrying B16 Bw4‐associated antigen which was not recognized by reagents against splits. Sequencing analysis showed new subtype termed B*3803. HLA class I sequence‐based typing (SBT) demonstrated that B*3803 co‐segregates with A*2608 Cw*1203, forming common...
The study of HLA class II polymorphism by direct exon 2 DNA sequencing analysis has been established to be a reliable and accurate high‐resolution typing procedure. This approach shows some advantages in relation previous methods, polymerase chain reaction using sequence‐specific oligonucleotides (PCR‐SSO) primers (PCR‐SSP), basically due the capability for complete sequenced genomic region, including non‐polymorphic motifs. DRB3 DQB1 based (SBT) unrelated bone marrow donor searching allowed...
HLA‐B14 serological subtyping is very limited probably due to the internal position of unique amino acid residue that differentiates B64 and B65 molecules. In order carry out an accurate B14 we have designed a semi‐nested PCR‐SSP procedure can differentiate B*1401 B*1402 in any HLA‐A, ‐B or ‐C antigen combination. A panel 133 B14‐positive 31 B14‐negative healthy unrelated Spanish individuals were studied. Additionally, 45 B14‐bearing haplotypes (‐A,‐B,‐C,‐DRB1,‐DRB3/DRB4/DRB5,‐DQA1,‐DQB1)...