A5002190584- Microfluidic and Bio-sensing Technologies
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Ultrasound and Cavitation Phenomena
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Capillary Electrophoresis Applications
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Protein purification and stability
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Proteins in Food Systems
- Fungal and yeast genetics research
- Microbial Metabolites in Food Biotechnology
- Cancer Research and Treatments
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Transgenic Plants and Applications
- Antimicrobial Peptides and Activities
- Tissue Engineering and Regenerative Medicine
- Nanopore and Nanochannel Transport Studies
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
Bioprocessing Technology Institute
2015-2025
Agency for Science, Technology and Research
2015-2025
National University of Singapore
2006-2008
United States Department of Agriculture
2001
One way to focus the diffuse energy of a sound field in liquid is by acoustically driving bubbles into nonlinear oscillation. A rapid and nearly adiabatic bubble collapse heats up interior produces intense concentration that able emit light (sonoluminescence) trigger chemical reactions (sonochemistry). Such phenomena have been extensively studied bulk liquid. We present here realization sonoluminescence sonochemistry created from confined within narrow channel polydimethylsiloxane-based...
Biotechnological advancements in carbon utilization, particularly CO2 gas fermentation, present a sustainable alternative to energy-intensive chemical catalysis. Clostridium carboxidivorans sp. 624, clostridial acetogen, employs the Wood-Ljungdahl pathway fix CO2, producing valuable acids and alcohols. In this study, we systematically investigate metabolic dynamics of CO2/H2 focusing on optimizing temperature media conditions enhance yield. Notably, batch fermentation at 25 °C achieved...
We present a study on achieving intense acoustic cavitation generated by ultrasonic vibrations in polydimethylsiloxane (PDMS) based microfluidic devices. The substrate to which the PDMS is bonded was forced into oscillation with simple piezoelectric transducer attached at 5 mm from device microscopic glass slide. operated 100 kHz driving voltages ranging between 20 V and 230 V. Close surface, pressure vibration amplitudes of up bar 400 nm were measured respectively. It found that this strong...
We report on an efficient ultrasound based technique for lysing Escherichia coli and Pichia pastoris with oscillating cavitation bubbles in integrated microfluidic system. The system consists of a meandering channel four piezoelectric transducers mounted glass substrate, the exposure gas pressure regulated by automatic control Controlled lysis bacterial yeast cells expressing green fluorescence protein (GFP) is studied high-speed photography microscopy, quantified real-time polymerase chain...
The ability to tolerate organic solvents is essential for bacteria producing these chemicals with high titer, yield, and productivity. As exemplified by 2-PE, bioproduction of 2-PE represents a promising alternative chemical synthesis plant extraction approaches, but its toxicity hinders successful large-scale microbial production.
Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between uptake various nutrients and their metabolic outcomes. During exponential phase, we observed sequential consumption order serine, aspartate glutamate as well complete key carbohydrate nutrients, glucose trehalose. Based on production rates measured metabolites, constraints-based genome-scale E. model then conducted elucidate utilization metabolism. The...
The overexpression of scFv antibody fragments in the periplasmic space Escherichia coli frequently results extensive protein misfolding and loss cell viability. Although folding factors such as Skp FkpA are often exploited to restore solubility functionality recombinant products, their exact impact on cellular metabolism during fragment expression is not clearly understood. In this study, we expressed scFvD1.3 E. BL21 evaluated overall physiological global gene changes upon or co-expression....
Abstract The presence of multiple copies plasmids in Escherichia coli could induce a complex cascade physiological changes known as the metabolic burden response. In this work, effect such plasmid on E. metabolism was investigated by constraint‐based genome‐scale flux modeling. We systematically applied three cellular objectives: (a) maximizing growth rate, (b) production, and (c) maintenance energy expenditure to quantify silico distributions. These simulated results were compared with...
Abstract Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range value-added products, particularly lipids. Sweetwater, methanol-free by-product fat splitting process, has emerged as alternative feedstock utilization glycerol. To further optimize sweetwater utilization, we compared growth and lipid production capabilities 21 oleaginous yeast strains under different conditions with various glycerol...
Drying technologies are often utilized to maximize microbial shelf-life stability of probiotics-based foods. However, these processes inadvertently induce stress on microorganisms and reduce probiotic viability. This work sought develop suitable protection strategies maintain viability powdered probiotics in different A formulation platform (set pre-existing/initial templates for application/adaptation various products) consisting six powder formulations was evaluated. Each template...
Phage therapy involves the application of lytic bacteriophages for treatment clinical infections but bacterial resistance may develop over time. Isolated from nosocomial infections, small colony variants (SCVs) are morphologically distinct, highly virulent strains that resistant to conventional antibiotics. In this study, SCVs was derived Pseudomonas aeruginosa exposed bacteriophage PB1 and these cells were subsequent phage infection by PB1. To elucidate mechanism SCV resistance, we...
The emergence of antibiotic resistant bacteria is one the biggest threats to human health worldwide. In 2017, World Health Organization listed world's most dangerous antibiotic-resistant or "superbugs", such as carbapenem-resistant Pseudomonas aeruginosa and Escherichia coli, indicating highest priority needs for new antibiotics. possibility that infectious diseases may soon be untreatable, due decreased efficacy, creates an urgent need novel alternative antimicrobials. Antimicrobial...
The phenomenon of stretched cells in the vicinity an oscillating bubble is investigated this work. Experiments reveal that a red blood cell can be up to five times its initial size towards end collapse laser-induced cavitation bubble. We hypothesize elasticity crucial for elongation. In order get insight physics involved, numerical simulations based on potential flow theory (with boundary element method) are performed. A simple membrane tension model elongating employed. observe stretching...
The incidence and prevalence of inflammatory disorders have increased globally, is projected to double in the next decade. Gut microbiome-based therapeutics shown promise ameliorating chronic inflammation. However, they are largely experimental, context- or strain-dependent lack a clear mechanistic basis. This hinders precision probiotics poses significant risk, especially individuals with pre-existing conditions. Molecules secreted by gut microbiota act as ligands several health-relevant...