A5026503374- Microtubule and mitosis dynamics
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Magnetic and Electromagnetic Effects
- CAR-T cell therapy research
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Protist diversity and phylogeny
MRC Laboratory of Molecular Biology
2023-2025
Centre National de la Recherche Scientifique
2019-2021
Université Paris Sciences et Lettres
2019-2021
Institut Curie
2019-2021
Sorbonne Université
2021
German Cancer Research Center
2008
Abstract Optimum protein function and biochemical activity critically depends on water availability because solvent thermodynamics drive folding macromolecular interactions 1 . Reciprocally, macromolecules restrict the movement of ‘structured’ molecules within their hydration layers, reducing available ‘free’ bulk therefore total thermodynamic potential energy water, or potential. Here, concentrated solutions such as cytosol, we found that modest changes in temperature greatly affect...
Elongator is a tRNA-modifying complex that regulates protein translation. Recently, moonlighting function of has been identified in regulating the polarization microtubule cytoskeleton during asymmetric cell division. induces symmetry breaking anaphase midzone by selectively stabilizing microtubules on one side spindle, contributing to downstream polarized segregation cell-fate determinants, and therefore fate determination. Here, we investigate how controls dynamics. binds both tip free...
Polarized cells rely on a polarized cytoskeleton to function. Yet, how cortical polarity cues induce polarization remains elusive. Here, we capitalized recently established designed 2D protein arrays ectopically engineer of virtually any interest during mitosis in various cell types. This enables direct manipulation signaling and the identification sufficient for polarization. Using this assay, dissected logic Par complex pathway, key regulator asymmetric division. We show that clustering...
The length of the mitotic spindle scales with cell size in a wide range organisms during embryonic development. Interestingly, C. elegans embryos, this goes along temporal regulation: larger cells speed up assembly and elongation. We demonstrate that, similarly fission yeast, dynamics adjust to size, which allows keep mitosis duration constant. Since prolongation was shown affect viability, may resemble mechanism regulate duration. further reveal how velocity elongation is regulated: coupled...
Mitotic spindle function depends on the precise regulation of microtubule dynamics and sliding. Throughout mitosis, both processes have to be orchestrated establish maintain stability. We show that during anaphase B elongation in Schizosaccharomyces pombe , sliding motor Klp9 (kinesin-6) also promotes growth vivo. In vitro, can enhance dampen growth, depending tubulin concentration. This indicates is able promote block subunit incorporation into lattice order set a well-defined velocity....
Epitranscriptomic has emerged as a fundamental control of gene expression. Nevertheless, the determination transcriptome-wide occupancy RNA modifications remains challenging. We have developed Rho-seq, an integrated pipeline detecting range through differential modification-dependent Rhodamine labeling. Using we confirm that reduction uridine to dihydrouridine targets tRNAs in E. coli. Unexpectedly, find D modification expands mRNAs fission yeast.The modified are enriched for...
Abstract Mitotic spindle function depends on the precise regulation of microtubule dynamics and sliding. Throughout mitosis, both processes have to be orchestrated establish maintain stability. We show that during anaphase B elongation in S. pombe , sliding motor Klp9 (kinesin-6) also promotes growth vivo. In vitro can enhance dampen growth, depending tubulin concentration. This indicates is able promote block subunit incorporation into lattice order set a well-defined velocity. Moreover,...