A5071482972- Viral-associated cancers and disorders
- Respiratory viral infections research
- Lymphoma Diagnosis and Treatment
- Circadian rhythm and melatonin
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Genetics, Aging, and Longevity in Model Organisms
- Infection Control and Ventilation
- Parvovirus B19 Infection Studies
- Spaceflight effects on biology
- Histiocytic Disorders and Treatments
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Diabetes and associated disorders
- Genetics, Bioinformatics, and Biomedical Research
- Light effects on plants
- Infection Control in Healthcare
- Antimicrobial agents and applications
- Inhalation and Respiratory Drug Delivery
- Heat shock proteins research
- Protein Structure and Dynamics
- COVID-19 epidemiological studies
- Polyomavirus and related diseases
- Contact Dermatitis and Allergies
- Herpesvirus Infections and Treatments
Imperial College London
2016-2025
The Francis Crick Institute
2025
Abstract Optimum protein function and biochemical activity critically depends on water availability because solvent thermodynamics drive folding macromolecular interactions 1 . Reciprocally, macromolecules restrict the movement of ‘structured’ molecules within their hydration layers, reducing available ‘free’ bulk therefore total thermodynamic potential energy water, or potential. Here, concentrated solutions such as cytosol, we found that modest changes in temperature greatly affect...
The mammalian cryptochrome proteins (CRY1 and CRY2) are transcriptional repressors most notable for their role in circadian feedback. Not all rhythms depend on CRY proteins, however, the promiscuous interactors that also regulate many other processes. In cells with chronic deficiency, protein homeostasis is highly perturbed, a basal increase cellular stress activation of key inflammatory signalling pathways. Here, we developed tools to delineate specific effects reduction, rather than better...
The within-host environment changes over circadian time and influences the replication severity of viruses. Genetic knockout transcription factors CRYPTOCHROME 1 2 ( CRY1 −/− / CRY2 ; CKO) leads to altered protein homeostasis chronic activation integrated stress response (ISR). adaptive ISR signalling pathways help restore cellular by downregulating synthesis in endoplasmic reticulum overloading or viral infections. By quantitative mass spectrometry analysis, we reveal that many recognition...
Activation-induced cytidine deaminase (AID), the enzyme responsible for induction of sequence variation in immunoglobulins (Igs) during process somatic hypermutation (SHM) and also Ig class switching, can have a potent mutator phenotype development lymphoma. Using various Epstein-Barr virus (EBV) recombinants, we provide definitive evidence that viral nuclear protein EBNA3C is essential EBV-infected primary B cells AID mRNA protein. lymphoblastoid cell lines (LCLs) established with EBV...
Mature human B cells infected by Epstein-Barr virus (EBV) become activated, grow, and proliferate. If the are ex vivo, they transformed into continuously proliferating lymphoblastoid cell lines (LCLs) that carry EBV DNA as extra-chromosomal episomes, express 9 latency-associated proteins, phenotypically resemble antigen-activated B-blasts. In vivo similar B-blasts can differentiate to memory (MBC), in which persistence is established. Three related viral proteins EBNA3A, EBNA3B, EBNA3C...
Article29 November 2021Open Access Source DataTransparent process CRYPTOCHROMES promote daily protein homeostasis David C S Wong orcid.org/0000-0002-1712-9527 MRC Laboratory of Molecular Biology, Cambridge, UK Search for more papers by this author Estere Seinkmane orcid.org/0000-0002-3636-4709 These authors contributed equally to work Aiwei Zeng orcid.org/0000-0003-0354-2529 Alessandra Stangherlin orcid.org/0000-0001-7296-1183 Nina M Rzechorzek orcid.org/0000-0003-3209-5019 Andrew D Beale...
EBV contributes to several types of human cancer. Some cancers and nonmalignant lymphoproliferative diseases involving T or NK cells contain EBV.
Abstract Viruses are vulnerable as they transmit between hosts, and we aimed to exploit this critical window. We found that the ubiquitous, safe, inexpensive biodegradable small molecule propylene glycol (PG) has robust virucidal activity. Propylene rapidly inactivates a broad range of viruses including influenza A, SARS‐CoV‐2 rotavirus reduces disease burden in mice when administered intranasally at concentrations commonly nasal sprays. Most critically, vaporised PG efficiently abolishes A...
Abstract The daily organisation of most mammalian cellular functions is attributed to circadian regulation clock-controlled protein expression, driven by cycles CRYPTOCHROME-dependent transcriptional feedback repression. To test this, we compared the proteome and phosphoproteome wild type CRY-deficient fibroblast cells. Strikingly, cells showed a two-fold increase in circadian-regulated proteins, phosphopeptides, K + transport. This was accompanied extensive remodelling overall, including...
Abstract Viruses are vulnerable as they transmit between hosts and we aimed to exploit this critical window. We found that the ubiquitous, safe, inexpensive biodegradable small molecule propylene glycol (PG) has robust virucidal activity. Propylene rapidly inactivates influenza, SARS-CoV-2 a broad range of other enveloped viruses, reduces disease burden in mice when administered intranasally at concentrations commonly nasal sprays. Most critically, aerosolized PG efficiently abolishes...
ABSTRACT Objectives To investigate whether the antimicrobial emollient Dermol 500 and its active components, benzalkonium chloride (BAK) chlorhexidine dihydrochloride (CD), exhibit virucidal activity thus informing is a suitable soap substitute for use during COVID19 pandemic, to combat increased incidence of work-related contact dermatitis in clinical settings that we report here. Methods Inactivation influenza A virus SARS-CoV-2 by independent combined components BAK CD was assessed...