A5060556060- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Nuclear Structure and Function
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Electron Spin Resonance Studies
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Plant and Fungal Interactions Research
Université Paris-Saclay
2019-2023
Centre National de la Recherche Scientifique
2005-2023
Institut de Biologie Intégrative de la Cellule
2019-2023
CEA Paris-Saclay
2021-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2023
Sorbonne Paris Cité
2017
Institut Jacques Monod
2017
Université Paris Cité
2017
Centre de Génétique Moléculaire
2005-2013
Aarhus University
2008-2013
Abstract Nuclear pore complexes (NPCs) have increasingly recognized interactions with the genome, as exemplified in yeast, where they bind transcribed or damaged chromatin. By combining genome-wide approaches live imaging of model loci, we uncover a correlation between NPC association and accumulation R-loops, which are genotoxic structures formed through hybridization nascent RNAs their DNA templates. Manipulating hybrid formation demonstrates that R-loop per se, rather than transcription...
Abstract Long non-coding RNAs (lncRNAs) contribute to the regulation of gene expression in response intra- or extracellular signals but underlying molecular mechanisms remain largely unexplored. Here, we identify an uncharacterized lncRNA as a central player shaping meiotic program fission yeast. We report that this regulatory RNA, termed mamRNA , scaffolds antagonistic RNA-binding proteins Mmi1 and Mei2 ensure their reciprocal inhibition fine tune mRNA degradation during mitotic growth....
Abstract Timely and accurate expression of the genetic information relies on integration environmental cues activation regulatory networks involving transcriptional post-transcriptional mechanisms. In fission yeast, meiosis-specific transcripts are selectively targeted for degradation during mitosis by EMC complex, composed Erh1, ortholog human ERH, YTH family RNA-binding protein Mmi1. Here, we present crystal structure Erh1 show that it assembles as a homodimer. Mutations amino acid...
Abstract Transcriptionally silent chromatin often localizes to the nuclear periphery. However, whether envelope (NE) is a site for post-transcriptional gene repression not well understood. Here we demonstrate that Schizosaccharomyces pombe Lem2, an NE protein, regulates nuclear-exosome-mediated RNA degradation. Lem2 deletion causes accumulation of precursors and meiotic transcripts de-localization engineered exosome substrate from does directly bind but instead interacts with...
Pericentromeric heterochromatin formation is mediated by repressive histone H3 lysine 9 methylation (H3K9Me) and its recognition HP1 proteins. Intriguingly, in many organisms, RNAi coupled to this process through poorly understood mechanisms. In Schizosaccharomyces pombe , the H3-K9 methyltransferase Clr4 protein 1 (HP1) ortholog Swi6 are critical for RNAi, whereas stimulates H3K9Me. addition endoribonuclease Dcr1, S. requires two interacting complexes, RITS complex, which contains an...
Production of messenger ribonucleoprotein particles (mRNPs) is subjected to quality control (QC). In Saccharomyces cerevisiae, the RNA exosome and its cofactors are part nuclear QC machinery that removes, or stalls, aberrant molecules, thereby ensuring only correctly formed mRNPs exported cytoplasm. The Ccr4-Not complex, which constitutes major S. cerevisiae cytoplasmic deadenylase, has recently been implied in exosome-related processes. Consistent with a possible function deletion mutation...
In fission yeast, meiosis-specific transcripts are selectively eliminated during vegetative growth by the combined action of YTH-family RNA-binding protein Mmi1 and nuclear exosome. Upon nutritional starvation, master regulator meiosis Mei2 inactivates Mmi1, thereby allowing expression meiotic program. Here, we show that E3 ubiquitin ligase subunit Not4/Mot2 evolutionarily conserved Ccr4-Not complex, which associates with promotes suppression in mitotic cells. Our analyses suggest Mot2...
Centromeric silencing and heterochromatin formation in Schizosaccharomyces pombe require the RNA interference (RNAi) machinery. Three factors that mediate this mechanism have been identified: 1) RNA-dependent polymerase complex RdRC, 2) Argonaute-containing RITS (RNA-induced initiation of transcriptional silencing) complex, 3) endoribonuclease Dicer ortholog Dcr1. S. mutants lacking a new factor described here, Ers1, are completely defective RNAi-dependent centromeric regions but,...
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic post-transcriptional levels. However, extent their regulatory potential underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in fission yeast long mamRNA carrying two independent cellular functions. On one hand, snR107 guides site-specific 25S...
The THO complex is involved in transcription, genome stability, and messenger ribonucleoprotein (mRNP) formation, but its precise molecular function remains enigmatic. Under heat shock conditions, mutants accumulate large protein-DNA complexes that alter the chromatin density of target genes (heavy chromatin), defining a specific biochemical facet powerful tool analysis. Here, we show heavy distribution dictated by gene boundaries promoter necessary sufficient to convey sensitivity these...
Heterochromatic gene silencing relies on combinatorial control by specific histone modifications, the occurrence of transcription, and/or RNA degradation. Once nucleated, heterochromatin propagates within defined chromosomal regions and is maintained throughout cell divisions to warrant proper genome expression integrity. In fission yeast Schizosaccharomyces pombe, Ccr4-Not complex partakes in silencing, but its relative contribution distinct domains role nucleation versus spreading have...
Abstract Heterochromatic gene silencing relies on combinatorial control by specific histone modifications, the occurrence of transcription, and/or RNA degradation. Once nucleated, heterochromatin propagates within defined chromosomal regions and is maintained throughout cell divisions to warrant proper genome expression integrity. The fission yeast Ccr4-Not complex has been involved in silencing, but its relative contribution distinct domains role nucleation versus spreading have remained...