A5020701044- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Oral microbiology and periodontitis research
- Advanced Glycation End Products research
- Metabolism and Genetic Disorders
- Digital Transformation in Industry
- Antimicrobial Peptides and Activities
- biodegradable polymer synthesis and properties
- Flexible and Reconfigurable Manufacturing Systems
- Advanced Proteomics Techniques and Applications
- Machine Learning in Bioinformatics
- Product Development and Customization
- Microbial metabolism and enzyme function
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
University of Göttingen
2015-2021
Fraunhofer Institute for Mechatronic Systems Design
2019
General Department of Preventive Medicine
2014
The second messenger cyclic di-adenosine monophosphate (c-di-AMP) is essential in the Gram-positive model organism Bacillus subtilis and related pathogenic bacteria. It controls activity of conserved ydaO riboswitch several proteins involved potassium (K+) uptake. We found that YdaO protein was among different bacteria provide evidence functions as a K+ transporter. Thus, we renamed gene KimA (K+ importer A). Reporter assays indicated expression beyond c-di-AMP-responsive kimA upstream...
Gram-positive bacteria synthesize the second messenger cyclic di-AMP (c-di-AMP) to control cell wall and potassium homeostasis secure integrity of their DNA. In firmicutes, c-di-AMP is essential for growth. The model organism Bacillus subtilis encodes three diadenylate cyclases two potential phosphodiesterases produce degrade c-di-AMP, respectively. Among cyclases, CdaA conserved in nearly all this enzyme seems be responsible that required homeostasis. Here, we demonstrate localizes membrane...
The Gram-positive model organism Bacillus subtilis produces the essential second messenger signaling nucleotide cyclic di-AMP. In B. and other bacteria, c-di-AMP has been implicated in diverse functions such as control of metabolism, cell division wall synthesis, potassium transport. To enhance our understanding multiple this messenger, we have studied consequences accumulation at a global level by transcriptome analysis. C-di-AMP affected expression about 700 genes, among them two major...
Potassium is the most abundant metal ion in every living cell. This essential due to its requirement for activity of ribosome and many enzymes but also because role buffering negative charge nucleic acids. As external concentrations potassium are usually low, efficient uptake intracellular enrichment necessary. The Gram-positive bacterium Bacillus subtilis possesses three transporters potassium, KtrAB, KtrCD, recently discovered KimA. In absence high-affinity KtrAB KimA, bacteria were unable...
In order to adjust changing environmental conditions, bacteria use nucleotide second messengers transduce external signals and translate them into a specific cellular response. Cyclic di-adenosine monophosphate (c-di-AMP) is the only known essential messenger. addition well-established role of this messenger in control potassium homeostasis, we observed that glutamate as toxic for c-di-AMP-free strain Gram-positive model bacterium Bacillus subtilis . work, isolated suppressor mutants allow...
Industry 4.0 promises numerous potentials in production, ranging from data-driven machine optimization to improving the entire production planning. The lack of transparency about effects and benefits is one key obstacles its introduction. Key Performance Indicators (KPIs) are a way capture changes production. This paper analyses typical KPIs represents their mathematical interconnection graph representation. In addition, structuring framework presented, which linked 4.0-related We conclude...
Utilization of energy-rich carbon sources such as glucose is fundamental to the evolutionary success bacteria. Glucose can be catabolized via glycolysis for feeding intermediary metabolism. The methylglyoxal synthase MgsA produces from glycolytic intermediate dihydroxyacetone phosphate. Methylglyoxal toxic, requiring stringent regulation activity. In Gram-positive bacterium Bacillus subtilis, an interaction with phosphoprotein Crh controls absence preferred sources, present in...
ABSTRACT In order to adjust changing environmental conditions, bacteria use nucleotide second messengers transduce external signals and translate them into a specific cellular response. Cyclic di-adenosine monophosphate (c-di-AMP) is the only known essential messenger. addition well-established role of this messenger in control potassium homeostasis, we observed that glutamate as toxic for c-di-AMP-free strain Gram-positive model bacterium Bacillus subtilis . work, isolated suppressor...