A5067518832- Legionella and Acanthamoeba research
- Bacterial biofilms and quorum sensing
- Advanced NMR Techniques and Applications
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Mass Spectrometry Techniques and Applications
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Signaling Pathways in Disease
- Amino Acid Enzymes and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Coenzyme Q10 studies and effects
- Heat shock proteins research
- PARP inhibition in cancer therapy
- Nerve injury and regeneration
- Modular Robots and Swarm Intelligence
- Calcium signaling and nucleotide metabolism
- Yersinia bacterium, plague, ectoparasites research
- RNA modifications and cancer
University of the Basque Country
2021-2024
Ikerbasque
2021-2024
Instituto Biofisika
2021-2024
Fundación Biofísica Bizkaia
2021-2024
Goethe University Frankfurt
2018-2023
University of Helsinki
2016-2021
CIC bioGUNE
2010-2020
Abstract Potassium homeostasis is vital for all organisms, but challenging in single-celled organisms like bacteria and yeast immobile plants that constantly need to adapt changing external conditions. KUP transporters facilitate potassium uptake by the co-transport of protons. Here, we uncover molecular basis transport this widely distributed family. We identify importer KimA from Bacillus subtilis as a member family, demonstrate it functions K + /H symporter report 3.7 Å cryo-EM structure...
The multisubunit Golgi-associated retrograde protein (GARP) complex is required for tethering and fusion of endosome-derived transport vesicles to the trans -Golgi network. Mutation leucine-967 glutamine in Vps54 subunit GARP responsible spinal muscular atrophy wobbler mouse, an animal model amyotrophic lateral sclerosis. crystal structure at 1.7 Å resolution mouse C-terminal fragment harboring leucine-967, conjunction with comparative sequence analysis, reveals that has a continuous...
Other than more widely used methods, the use of styrene maleic acid copolymers allows direct extraction membrane proteins from lipid bilayer into SMALPs keeping it in its native surrounding.
Abstract P-type ATPases ubiquitously pump cations across biological membranes to maintain vital ion gradients. Among those, the chimeric K + uptake system KdpFABC is unique. While ATP hydrolysis accomplished by ATPase subunit KdpB, has been assumed be transported channel-like KdpA. A first crystal structure uncovered its overall topology, suggesting such a spatial separation of energizing and transporting units. Here, we report two cryo-EM structures 157 kDa, asymmetric complex at 3.7 Å 4.0...
Asparaginyl endopeptidases (AEPs) catalyze head‐to‐tail backbone cyclization of naturally occurring cyclic peptides such as cyclotides, and have become an important peptide‐engineering tool for macrocyclization peptide ligation. Here, we report efficient protein ligation in trans by mimicking AEP without any excess reactants. We demonstrate a practical application segmental isotopic labeling NMR studies single‐domain globular refolding step using the recombinant prepared from Escherichia...
Abstract Cyclic di-AMP is the only known essential second messenger in bacteria and archaea, regulating different proteins indispensable for numerous physiological processes. In particular, it controls various potassium osmolyte transporters involved osmoregulation. Bacillus subtilis , K + /H symporter KimA of KUP family inactivated by c-di-AMP. sustains survival at limitation low external pH mediating ion uptake. However, elevated intracellular concentrations, further accumulation would be...
The covalent attachment of adenosine monophosphate (AMP) to proteins, a process called AMPylation (adenylylation), has recently emerged as novel theme in microbial pathogenesis. Although several AMPylating enzymes have been characterized, the only known virulence protein with de-AMPylation activity is SidD from human pathogen Legionella pneumophila. de-AMPylates mammalian Rab1, small GTPase involved secretory vesicle transport, thereby targeting host for inactivation. molecular mechanisms...
Abstract Bacterial competition is a significant driver of toxin polymorphism, which allows continual compensatory evolution between toxins and the resistance developed to overcome their activity. R earrangement h ot s pot (Rhs) proteins represent widespread example polymorphism. Here, we present 2.45 Å cryo-electron microscopy structure Tse5, an Rhs protein central Pseudomonas aeruginosa type VI secretion system-mediated bacterial competition. This structural insight, coupled with extensive...
Glial cell line-derived neurotrophic factor (GDNF) is a ligand that activates, through co-receptor GDNF family receptor alpha-1 (GFRα1) and tyrosine kinase "RET", several signaling pathways crucial in the development sustainment of multiple neuronal populations. We decided to study whether non-mammalian orthologs these three proteins have conserved their function: can they activate human counterparts? Using baculovirus expression system, we expressed purified Danio rerio RET, its binding...
How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS one type III-B system, regulated by the transmembrane anti-σ DdvA its cognate extracytoplasmic function (ECF) σ DdvS, can defend
AMPylation, the post-translational modification with adenosine monophosphate (AMP), is catalyzed by effector proteins from a variety of pathogens. Legionella pneumophila thus far only known pathogen that, in addition to encoding an AMPylase (SidM/DrrA), also encodes deAMPylase, called SidD, that reverses SidM-mediated AMPylation vesicle transport GTPase Rab1. DeAMPylation N-terminal phosphatase-like domain SidD. Here, we determined crystal structure full length SidD including uncharacterized...
Envelope glycoproteins from genetically-divergent virus families comprise fusion peptides (FPs) that have been posited to insert and perturb the membranes of target cells upon activation virus-cell reaction. Conserved sequences rich in aromatic residues juxtaposed external leaflet virion-wrapping are also frequently found viral glycoproteins. These membrane-proximal regions (MPERs) implicated promotion membrane restructuring event required for proceed, hence, proposed supplementary FPs....
Knots have attracted scientists in mathematics, physics, biology, and engineering. Long flexible thin strings easily knot tangle as experienced our daily life. Similarly, long polymer chains inevitably tend to get trapped into knots. Little is known about their formation or function proteins despite >1,000 knotted identified nature. However, these protein knots are not mathematical with backbone polypeptide because of open termini, the presence a “knot” depends on algorithm used...
Abstract Cyclic di-AMP is the only known essential second messenger in bacteria and archaea, regulating different proteins indispensable for numerous physiological processes. In particular, it controls various potassium osmolyte transporters involved osmoregulation. Bacillus subtilis , K + /H symporter KimA of KUP family inactivated by c-di-AMP. sustains survival at limitation low external pH mediating ions uptake. However, elevated intracellular concentrations, further accumulation would be...
Summary Clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonucleases have revolutionized biotechnology for their potential as programmable genome editors. Yet, most natural nucleases and variants limitations. Here, we report a fully synthetic CRISPR-associated (Cas) nuclease (α-synCas) designed by Ancestral Sequence Reconstruction (ASR) that displays set of robust distinct targeting properties, not found in any other known CRISPR-Cas Class 2 system. We show...