A5024456106- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- Fibroblast Growth Factor Research
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Wound Healing and Treatments
- Cancer Cells and Metastasis
- Advancements in Transdermal Drug Delivery
- Mass Spectrometry Techniques and Applications
- Single-cell and spatial transcriptomics
- Gold and Silver Nanoparticles Synthesis and Applications
- Gender, Labor, and Family Dynamics
- Cancer Mechanisms and Therapy
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Phagocytosis and Immune Regulation
- Machine Learning and ELM
- RNA Interference and Gene Delivery
University of Virginia
2022-2025
Indiana University Bloomington
2020-2022
Baylor University
2016
Bidirectional cell-cell communication involving exosome-borne cargo such as miRNA has emerged a critical mechanism for wound healing. Unlike other shedding vesicles, exosomes selectively package by SUMOylation of heterogeneous nuclear ribonucleoproteinA2B1 (hnRNPA2B1). In this work, we elucidate the significance exosome in keratinocyte-macrophage crosstalk following injury. Keratinocyte-derived were genetically labeled with GFP-reporter (Exoκ-GFP) using tissue nanotransfection (TNT), and...
The tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC) plays a key role progression and response to therapy. dense PDAC stroma causes hypovascularity, which leads hypoxia. Here, we showed that hypoxia drives long-lasting epithelial-mesenchymal transition (EMT) primarily through positive-feedback histone methylation-MAPK signaling axis. Transformed cells preferentially underwent EMT hypoxic regions multiple model systems. Hypoxia drove cell autonomous cells, which, unlike...
ABSTRACT Epithelial-mesenchymal transition (EMT) occurs heterogeneously among malignant carcinoma cells to promote chemoresistance. Identifying the signaling pathways involved will nominate drug combinations chemoresponse, but cell population-level studies are inherently fraught, and single-cell transcriptomics limited indirect ontology-based inferences. To understand EMT heterogeneity at a protein level, we combined iterative immunofluorescence imaging of pancreas cancer tumors mutual...
The masses of particles in a bovine milk extracellular vesicle (EV) preparation enriched for exosomes were directly determined the first time by charge detection mass spectrometry (CDMS). In CDMS, both mass-to-charge ratio (m/z) and z are simultaneously individual particles, enabling determinations that far beyond limit (∼1.0 MDa) conventional (MS). Particle charges span wide range from m ∼ 2 to ∼90 MDa 50 ∼1300 e (elementary charges) highly dependent upon conditions used extract isolate...
<div>Abstract<p>The tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC) plays a key role progression and response to therapy. The dense PDAC stroma causes hypovascularity, which leads hypoxia. Here, we showed that hypoxia drives long-lasting epithelial–mesenchymal transition (EMT) primarily through positive-feedback histone methylation–MAPK signaling axis. Transformed cells preferentially underwent EMT hypoxic regions multiple model systems. Hypoxia drove cell...
Unresolved inflammation compromises diabetic wound healing. Recently, we reported that inadequate RNA packaging in murine wound-edge keratinocyte-originated exosomes (Exoκ) leads to persistent [Zhou, X. ACS Nano 2020, 14(10), 12732−12748]. Herein, use charge detection mass spectrometry (CDMS) analyze intact Exoκ isolated from a 5 day old tissue of mice and heterozygous nondiabetic littermate control group. In CDMS, the (z) mass-to-charge ratio (m/z) individual exosome particles are measured...
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<p>Supplementary Methods, Tables, References</p>
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