Abstract In this study, we assessed the genetic relatedness of 11 representative Pseudomonas syringae pv. ( Pss ) strains isolated in Serbia from different herbaceous and woody plant species, as well one reference strain, using various molecular tools such repetitive element palindromic PCR, arbitrarily primed insertion sequences 50 PCR (IS50‐PCR) multilocus sequence analysis (MLSA). Their virulence traits were also by inoculating detached pear lilac leaves, immature cherry fruitlets. The...

The development of biological pesticides is rapidly becoming an integral aspect pest management in sustainable agriculture. This study was conducted to evaluate the effectiveness Bacillus halotolerans strain B33 against three common seedborne fungal pathogens-Fusarium graminearum, Alternaria alternata, and Aspergillus flavus. identity determined using 16S rRNA tuf gene sequences. Commercial wheat, barley, oat, rye seeds were artificially infected by isolates then treated with overnight...

In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite- F-CSZ, nature F-CNZ and gelatin- F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as alternative of conventional pesticides. By wanted enable water solubility ensure prolonged efficacy oil. Biological activity designed CEO tested potato tuber moth...

Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) one of the main potato growing areas Serbia (Bačka region, Vojvodina). The percentage infected plants reached 40 to 70%, with 10.5 44.7% yield reductions. From fields, out 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent blackleg for first time (29 isolates). Dickeya dianthicola a less causative bacterium, which also...

The aim of this work was to identify and characterize the pectolytic bacteria responsible for emergence bacterial soft rot on two summer cabbage hybrids (Cheers F1 Hippo F1) grown in Futog locality (Bačka, Vojvodina), known five-century-long tradition cultivation Serbia. Symptoms manifesting as lesions outer head leaves were observed during August 2021, while inner tissues macerated, featuring cream black discoloration. As affected tissue decomposed, it exuded a specific odor. Disease...

The present study provides insight into the diversity of 147 Xanthomonas campestris pv. (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and winter oilseed rape crop napus , collected different regions in Serbia 2014. XCF/XCR pathovar‐specific primer set was used for fast preliminary identification. In repetitive sequence‐based PCR (BOX, ERIC REP) all isolates, a higher level genetic found compared to other...

Unusual blight-like symptoms appeared on highbush blueberry plants in Serbia during August 2015 and infected showed browning reddening of leaves, drying foliage brown discolouration internal vascular stem tissues. The objective this study was to isolate confirm a causal agent the disease. Five diseased (2-year-old), with visible wood, were collected for isolation potato dextrose agar (PDA). Morphological analysis selected fungal isolates presence abundant black, round oblong, or irregularly...

Potato blackleg is frequently observed on the production fields in Bačka region of Vojvodina province, which one largest potato-growing areas Serbia. This disease usually occurs during June and July. In July 2020, symptoms form stem necrotic lesions, vascular discoloration, hollow stems, wilting whole plants were noted potato cultivar VR808 a field 28 ha size located Maglić village (GPS coordinates 45.349325 N, 19.542768 E). Disease incidence was estimated at 20-25%. Isolations performed...

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Numerous scientific research studies all over the world have addressed problem of agriculture in 21st century as being particularly sensitive to climate change, which has caused phytopathogenic bacteria spread. Therefore, there is a clear and urgent need contain this kind risk agricultural production (both conventional organic farming). The objective study was determine antibacterial activity 30 essential oils (EOs) against three harmful plant pathogenic importance, Erwinia amylovora,...

Abstract In May 2016, an unusual appearance of leaf spot (water‐soaked, brown‐purple, round to angular surrounded with yellow halos) was observed on the leaves wild cherry specimens grown in Rimski Šančevi, Vojvodina (North Serbia). The causal pathogen isolated from diseased Nutrient Agar supplemented 5% sucrose and identified as Pseudomonas cerasi based multilocus sequence analysis (MLSA). PCR amplification sequencing four housekeeping genes— gapA , gltA rpoD gyrB —showed 100% ( ), 99.81% )...

Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown a vegetable farm in Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG 5 , REP, SERE) randomly amplified polymorphic DNA-PCR M13 primer revealed...

The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots carrot (Daucus carota L.). Disease symptoms appeared at end June form chlorosis some leaves, which became intensely red one week later, while previously healthy from same branch becme chlorotic. A few days all infected leaf were red. Infected plants also had slower growth compared to ones with fewer branches developed. less developed, seared, or gummy without...

The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, 2018 was identified characterized. Symptoms appeared the form water-soaked lesions on leaves specific odour. Disease incidence ranged from 20% to 30%. pathogen isolated nutrient agar (NA), King?s B crystal violet pectate (CVP) media. Eight creamy-white, round convex bacterial isolates, which produced characteristic pits CVP medium were taken as representative. They gram...

Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet production significantly increased new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 2019, occurrence of bacterial symptoms was observed on a newly planted plantations two localities, Zitoradja (Southern region) Karavukovo (Northern region-Vojvodina). Typical P. syringae...

Abstract Winter oilseed rape has recently been described as a new host for the phytopathogenic bacterium Xanthomonas campestris pv. (Xcc) in Serbia. In order to provide information about genetic structure of this bacterium's population and its pathogenic features on crop, collection 65 winter Xcc isolates obtained over 5‐year period (2014–2018) from different Serbian localities was examined. The presence intrapathovar diversity within tested confirmed by repetitive element PCR (rep‐PCR),...

Abstract Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB‐1 (R3/B2/PIIB‐1) have significantly compromised potato production in Serbia. During 6 years monitoring (2013–2018) among 3,524 tuber samples, 344 were found positive for disease. R . R3/B2/PIIB‐1 was isolated from seven cultivars 12 monitored, and five localities 17 monitored. Cultivar Lady Claire to highest disease frequency (31.98%). A total 78 isolates identified ‐specific...

Abstract The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia Montenegro). Multilocus sequence typing analysis of sequences nine housekeeping genes revealed homology between tested Xap strains as well with European this bacterium. share same haplotype (Haplotype I) Italy, France, Spain, United States, Australia Brazil. single nucleotide change (G ↔ C)...

In August 2020, the unusual appearance of crown gall symptoms was observed on tobacco plants (hybrid PVH2310) grown in fields Golubinci (Srem district, Serbia) locality. The causal agent isolated from galls located roots formed circular, convex, and glistening light blue colonies, then dark to olive-green-colored bacterial colonies a semi-selective D1 medium. Molecular analysis based multiplex PCR multi-locus sequence (MLSA) using concatenated sequences atpD, dnaK, glnA, rpoB genes as well...

Infections with phytoplasma present one of the most significant biotic stresses influencing plant health, growth, and production. The ‘Candidatus Phytoplasma solani’ infects a variety species. This pathogen impacts physiological morphological characteristics plants causing stunting, yellowing, leaf curling, other symptoms that can lead to economic losses. aim this study was determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita dill (Anethum graveolens L.)...