Abstract In this study, we assessed the genetic relatedness of 11 representative Pseudomonas syringae pv. ( Pss ) strains isolated in Serbia from different herbaceous and woody plant species, as well one reference strain, using various molecular tools such repetitive element palindromic PCR, arbitrarily primed insertion sequences 50 PCR (IS50‐PCR) multilocus sequence analysis (MLSA). Their virulence traits were also by inoculating detached pear lilac leaves, immature cherry fruitlets. The...

The development of biological pesticides is rapidly becoming an integral aspect pest management in sustainable agriculture. This study was conducted to evaluate the effectiveness Bacillus halotolerans strain B33 against three common seedborne fungal pathogens-Fusarium graminearum, Alternaria alternata, and Aspergillus flavus. identity determined using 16S rRNA tuf gene sequences. Commercial wheat, barley, oat, rye seeds were artificially infected by isolates then treated with overnight...

Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) one of the main potato growing areas Serbia (Bačka region, Vojvodina). The percentage infected plants reached 40 to 70%, with 10.5 44.7% yield reductions. From fields, out 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent blackleg for first time (29 isolates). Dickeya dianthicola a less causative bacterium, which also...

The aim of this work was to identify and characterize the pectolytic bacteria responsible for emergence bacterial soft rot on two summer cabbage hybrids (Cheers F1 Hippo F1) grown in Futog locality (Bačka, Vojvodina), known five-century-long tradition cultivation Serbia. Symptoms manifesting as lesions outer head leaves were observed during August 2021, while inner tissues macerated, featuring cream black discoloration. As affected tissue decomposed, it exuded a specific odor. Disease...

Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only the north (Vojvodina Province), up to 1,500 ha. June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent greasy spots with yellow margins) were observed first time, cultivated at experimental fields near Backi Petrovac. Since then, regularly on beginning of growing seasons during rainy weather, disease incidence ranging from 5 20%....

Potato blackleg is frequently observed on the production fields in Bačka region of Vojvodina province, which one largest potato-growing areas Serbia. This disease usually occurs during June and July. In July 2020, symptoms form stem necrotic lesions, vascular discoloration, hollow stems, wilting whole plants were noted potato cultivar VR808 a field 28 ha size located Maglić village (GPS coordinates 45.349325 N, 19.542768 E). Disease incidence was estimated at 20-25%. Isolations performed...

Abstract In this study, the susceptibility of different sweet (19) and sour cherry (3) cultivars (cvs.) to bacterial pathogens Pseudomonas syringae pv. P. s. morsprunorum race 1 causing canker (sensu stricto) leaf spot was evaluated. Susceptibility examined by artificial inoculations 1‐year‐old rooted trees, which revealed all cvs. both pathovars . level differed among cvs.; Katalin, Linda, Summit, New Star Bigarreau Burlat were highly susceptible, Érdi bôtermô Drogan's Yellow, Carmen,...

Pseudomonas syringae pvs., isolated from different cultivars of sweet cherry grown in several locations Serbia, were characterized and compared with strains collected earlier sour oil pumpkin growing this region, as well reference P. pv.morsprunorum race 1 CFBP2119, s. pv.lachrymans 765R pv.syringae H-1.By employing LOPAT GATTa tests, isolates identified 1. Simultaneous detection syringomycin synthesis (syrB) secretion (syrD) genes multiplex-polymerase chain reaction (m-PCR) was used for...

Abstract In May 2016, an unusual appearance of leaf spot (water‐soaked, brown‐purple, round to angular surrounded with yellow halos) was observed on the leaves wild cherry specimens grown in Rimski Šančevi, Vojvodina (North Serbia). The causal pathogen isolated from diseased Nutrient Agar supplemented 5% sucrose and identified as Pseudomonas cerasi based multilocus sequence analysis (MLSA). PCR amplification sequencing four housekeeping genes— gapA , gltA rpoD gyrB —showed 100% ( ), 99.81% )...

In recent years, sweet and sour cherry production in Serbia has increased. Under Serbian agroecological conditions, pathogens causing leaf diseases threaten the success of production. period 2012-2019, health status cherries was monitored more than 30 locations. Depending on system, following were identified: Blumeriella jaapii, Wilsonomyces carpophilus, Mycosphaerella cerasella, Phoma prunicola, Podosphaera clandestina Pseudomonas syringae pv. morsprunorum race 1. Leaf caused premature...

Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates colony morphology typical for genus Xanthomonas characterized pathogenicity, biochemical, serological, molecular assays. All reacted positive a polymerase chain reaction (PCR) using XcpM1 XcpM2 primers specific hortorum pv. pelargonii. pathogenicity tests on Pelargonium zonale (leaf stem inoculation), all caused starting 2 days after...

Pseudomonas syringae pvs., isolated from sweet cherry grown on different localities in Serbia, were genetically characterized using RAPD analysis. Four out of eleven tested primers (SPH1, DJP 17, DJ 15, and 16) selected the basis differences between isolates within two pathovars - morsprunorum race 1. Cumulative analysis indicated heterogeneity population both groups isolates, revealing four patterns each group. showed up to 24% among pv. as well 41% comparison with reference strain KFB0103...

The effectiveness of medicinal plants is mainly associated with their active constituents, but one the major quality problems frequently encountered high trace metals content that can be to extensive pollution environment where grow. Therefore aim this research was evaluate Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn and As in selected used plants, including chicory, broadleaf, common comfrey dandelion. plant material collected from wild habitats area highly developed power activity during summer...

Erwinia amylovora, the causal agent of fire blight (FB) on fruit trees and ornamental plants, rapidly spread across eastern Mediterranean countries in early 1980s. This quarantine bacterium probably arrived southern parts former Yugoslavia (now FYR Macedonia) from Greece. Based symptoms, isolation identification data, it was concluded that amylovora pear drying Macedonia (1989). It first experimental confirmation a presence E. territory Yugoslavia. The also proved Serbia same year. In Bosnia...

Summary Monilinia laxa Aderh. and Ruhl. is the predominant causal agent of brown rot disease stone fruit orchards, especially sweet cherries. The objective this study was to identify reaction in response nine genotypes cherry, with different pomological properties, against rot. These were harvested at commercial maturity from orchard Fruit Research Institute Rimski Šančevi. studied showed significant differences terms occurrence on fruits, both under artificial inoculation infection field....

The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, 2018 was identified characterized. Symptoms appeared the form water-soaked lesions on leaves specific odour. Disease incidence ranged from 20% to 30%. pathogen isolated nutrient agar (NA), King?s B crystal violet pectate (CVP) media. Eight creamy-white, round convex bacterial isolates, which produced characteristic pits CVP medium were taken as representative. They gram...

Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet production significantly increased new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 2019, occurrence of bacterial symptoms was observed on a newly planted plantations two localities, Zitoradja (Southern region) Karavukovo (Northern region-Vojvodina). Typical P. syringae...

The effects of plant growth promoting (PGP) bacteria on soybean and yield were tested in field conditions using four treatments: (A) Bradyrhizobium japonicum strain 526, combined with cell-free supernatants (CFS) Bacillus sp. Q10 Pseudomonas chlororaphis Q16; (B) B. 526 + Q10; (C) P. Q16 (D) commercial fertilizer containing japonicum, which served as a control. average values dry weight per nodule shoot had the maximum B treatment. In shoots collected at flowering stage, nitrogen carbon...

Xanthomonas arboricola pv. pruni (Xap) causes bacterial spot of leaves and fruits, defoliation, fruit dropping twigs, branches or trunk cankers in most cultivated ornamental Prunus species. The bacterium is listed as an EPPO (European Mediterranean Plant Protection Organisation) A2 quarantine pathogen. Xap was first detected 2019 on peach [Prunus persica L., unknown cultivar (cv.)] a 13-year-old orchard located Irig (GPS: 45°6'10.538'' N, 19°54'8.04'' E), with disease incidence 10-20%....