Mitragyna speciosa (Ms), M. diversifolia (Md), hirsuta (Mh) and rotundifolia (Mr) were investigated for phytochemicals by GC-MS GC-FID, cytotoxicity genotoxicity testing MTT comet assay, biological activity examination through gene expression of human µ, δ, κ, nociceptin opioid receptors qRT-PCR. The substances mitragynine, 7-hydroxymitragynine, mitraphylline found in all studied species, and, first time, rhynchophylline was Mr, Md, Mh Mr. assays the ethanol hexane leaf extracts on PBMCs...

The multicolor banding (MCB/mBAND) technique provides a unique opportunity to characterize intrachromosomal rearrangements and determine chromosomal breakpoints. Until recently, MCB probes have only been available for human some murine chromosomes. Generation of chromosomes other species, useful required in many cytogenetics research fields, was limited by technical difficulties. are established chromosome microdissection followed whole genomic DNA amplification. However, unambiguous...

The chromosomal homologies of human (Homo sapiens = HSA) and silvered leaf monkey (Trachypithecus cristatus TCR) have been previously studied by classical chromosome staining fluorescence in situ hybridization (FISH) applying chromosome-specific DNA probes all chromosomes the 1980s 1990s, respectively. However, as resolution these techniques is limited we used multicolor banding (MCB) at an ~250-band level, other selected to establish a detailed map TCR. Therefore it was possible precisely...

α-EG is a unique substance that was first found in the leaves and fruits of Morinda citrifolia (Mc) growing Thailand using GC-MS at 52.33% 54.12%. It then concentrated its abundance quantified, along with pinoresinol, via GC, compared to standards leaves, ufp, rfp, rawfs, seeds. which have collagen stimulating, skin whitening, an inhibitory effect on wrinkle formation, were different concentrations amounts. Three five Mc part extracts tested NHDF for gene expression related aforementioned...

The first chromosomal characteristic of nucleolar organizer regions/NORs and karyological analysis the royal knifefish (Chitala blanci) from Mekong River, northeast Thailand, were studied. Kidney cell samples taken one male female fish. mitotic chromosome preparations done directly kidney cells. Conventional Ag-NOR staining techniques applied to stain chromosomes. results showed that diploid number C. blanci was 2n=42, fundamental numbers (NF) 42 in both female. types chromosomes 12 large...

The present study reports the first finding of NOR (nucleolar organizer region) polymorphism and chromosome analysis in John's snapper (Lutjanus johnii) from Thailand.Kidney cell samples were taken four male female fish.Mitotic preparations conducted using standard squash technique, as well directly kidney cells.Metaphase spreads performed on microscopic slides then air-dried.Conventional Ag-NOR banding techniques applied to stain chromosome.The results showed that diploid number L. johnii...

Our knowledge is the first report on karyotypic study of four toad species (genus Bufo) in Thailand namely; large-eared (Bufo macrotis Boulenger 1887), Indochinese dwarf parvus common Indian melanostictus Schneider 1799) and river giant asper Gravenhorst 1829). Blood samples were taken from 5 males females each species. After standard whole blood T-lymphocyte culture presence colchicine, metaphase spreads performed microscopic slides air-dried. Conventional staining, G-banding C-banding...

The first cytogenetics of the Malayan snail-eating turtle (Malayemys macrocephala) from Chi river basin, Khon Kaen Province, Thailand, were studied. Blood samples taken two male and female turtles. Standard T-lymphocyte cell culture at 26°C for 96 h was applied. mitotic chromosomes harvested by colchicine-hypotonic-fixation-air drying technique. Conventional staining GTG-banding techniques applied to stain with 20% Giemsa's solution. Results showed that number diploid 2n=50, while...

Based on the long history of medicinal use Thunbergia laurifolia, Clerodendrum disparifolium and Rotheca serrata, extract formulations these species: T. laurifolia C. disparifolium; R. serrata; called formulas 1, 2 3, were created for detoxification testing to take more advantage each species.The objective this study is estimate detoxifying effects studied human cell tissue culture as a preclinical trial.The major phytochemicals derived by GC-MS. The efficacy in cells DNA levels MTT comet...

The six Dioscorea species, D. brevipetiolata, bulbifera, depauperata (Dd), glabra (Dg), pyrifolia and hamiltonii were analyzed for phytochemicals, toxicity in PBMCs, biological activity two cancer cell lines by MTT comet assays, pesticide efficiency. Via GC-MS, lidocaine was found to be the predominant compound of studied species. To confirm systematics, also lower amounts 11 assay showed no all key result that ethanol extracts Dd Dg violently broke DNA into pieces. Biological these species’...

Comparative chromosome banding analysis and/or fluorescence in situ hybridization (FISH) studies are established approaches to compare human and ape chromosomes.FISH is a relatively new not routinely applied method very well suited provide better understanding of the evolutionary history primate phylogeny.Here multicolor (MCB)-applying probes derived from Homo sapiens were used analyze chromosomes Thai crab-eating macaque (Macaca fascicularis).The results agree with those previous other...

Comparative chromosome banding analysis and/or fluorescence in situ hybridization (FISH) studies are established approaches to compare human and ape chromosomes. FISH-banding is a relatively new not routinely applied method suited very well provide better understanding of the evolutionary history primate phylogeny. Here multicolor (MCB) applying probes derived from Homo sapiens was used analyze chromosomes Thai crab-eating macaque (Macaca fascicularis). The results agree with those previous...

This research was the first cytogenetic study of Malayan porcupine (Hystrix brachyura) from Songkla Zoo, Thailand. Blood samples were taken two males and females then subjected to standard whole blood T-lymphocyte culture. The harvested by colchicine-hypotonic-fixation-air-drying technique followed conventional staining G-banding with Giemsa's. results showed that diploid number 2n=66, fundamental (NF) 127 128 in male female, respectively. autosomes consist 6 large submetacentric, 20...

As an endangered species in Thailand, wild animal of the jungle cat (Felis chaus) was selected for karyological study. Blood samples were taken from 2 males and females. After standard whole blood lymphocyte culture presence colchicine, metaphase spreads performed on microscopic slides air-dried. Conventional staining, G-banding high-resolution staining technique applied to stain chromosomes. The results showed that 2n (diploid) 38, fundamental number (NF) 72 male female. There are 6...

Introduction: Plants containing β-sitosterol and oleamide are important for various diseases. So, Dillenia indica, D. obovata, pentagyna were investigated phytochemicals, cytotoxicity genotoxicity levels on peripheral blood mononuclear cells (PBMCs) Hela cells. The protective effect of extract a HepG2 cell line was also investigated. Methods: Gas chromatography-mass spectrometry (GC-MS) high-performance liquid chromatography (HPLC) used phytochemical analysis. 3-(4,5-dimethylthiazol-2-...