Abstract. Seree W, Supiwong Nuntaporn G, Ditcharoen S, Donbundit N, Seetapan K, Tongnunui Juntharat Tanomtong A. 2025. Comparative study on karyotypes of three Mastacembelus species (Synbranchiformes: Mastacembelidae) from Thailand. Biodiversitas 26: 246-254. Karyotype or chromosomal is necessary for some cases fish taxonomy. The chromosomes named Zig-zag eel armatus, Fire erythrotaenia, and Tire track favus using conventional Giemsa staining Ag-Nucleolar Organizer Region (NOR) banding...

Abstract Background Java combtail fish Belontia hasselti (Cuvier, 1831), a member of the Osphronemidae family, inhabits lakes and rivers throughout Southeast Asia Sri Lanka. Previous cytogenetic research revealed it possesses diploid chromosome number 48 chromosomes with female-heterogametic ZZ/ZW sex system, where W is distinguishable as only metacentric element in complement. Female-heterogametic systems seem to be otherwise surprisingly rare highly diverse order Perciformes and,...

The barbels of the subfamilies ´Poropuntinae´ and Smiliogastrinae within family Cyprinidae play a significant role as food source for fish in artisanal fisheries are highly valued ornamental Thailand. In this study, we employed both conventional molecular cytogenetics to analyze karyotype 15 species from two cyprinid lineages. All analyzed had diploid chromosome number 2n = 50. Despite sharing same 2n, our analyses revealed species-specific distribution patterns mapped microsatellite motifs...

Channid fishes, commonly referred to as “snakeheads”, are currently very important in Asian fishery and aquaculture due the substantial decline natural populations because of overexploitation. A large degree chromosomal variation has been found this family, mainly through use conventional cytogenetic investigations. In study, we analyzed karyotype structure distribution 7 repetitive DNA sequences several Channa species from different Thailand river basins. The aim study was investigate...

Synbranchidae or swamp eels are fishes belonging to the order Synbranchiformes that occur in both freshwater and occasionally brackish. They worldwide distributed tropical subtropical rivers of four different continents. A large degree chromosomal variation has been found this family, mainly through use conventional cytogenetic investigations. Inside group, a still almost unexplored species under point view is Asian eel Monopterus albus, widely throughout Asia. Here, we tested hypothesis...

The Labeoninae subfamily is a highly diversified but demonstrably monophyletic lineage of cyprinid fishes comprising five tribes and six incertae sedis genera. This widely distributed assemblage contains some 48 genera around 480 recognized species in freshwaters Africa Asia. In this study, the karyotypes other chromosomal properties found Thailand Labeo chrysophekadion (Labeonini) Epalzeorhynchos bicolor , munense Henicorhynchus siamensis Thynnichthys thynnoides (´Osteochilini´) were...

In the present study, conventional and molecular cytogenetic studies were performed in naked catfish Mystus bocourti (Siluriformes, Bagridae). Besides Giemsa staining, fluorescence situ hybridization (FISH) using nine classes of repetitive DNAs namely 5S 18S rDNAs, U2 snRNA, microsatellites (CA)15 (GA)15, telomeric repeats, retrotransposable elements Rex1, 3 6. was also performed.M. had 2n = 56 chromosomes with a karyotype composed by 11 m + sm 6 st/a fundamental number (NF) equal to 100...

The genus Clarias (Clariidae, Siluriformes) contains at least 61 species naturally spread over vast regions of Asia, India and Africa. However, have also been introduced in many different countries represent the most widespread catfishes world. These fishes are known as "walking catfishes" due to their ability move land. A large degree chromosomal variation has previously found this family, mainly using conventional cytogenetic investigations, with diploid chromosome numbers ranging between...

The representatives of cyprinid lineage 'Poropuntiinae' with 16 recognized genera and around 100 species form a significant part Southeast Asian ichthyofauna. Cytogenetics are valuable when studying fish evolution, especially the dynamics repetitive DNAs, such as ribosomal DNAs (5S 18S) microsatellites, that can vary between species. Here, karyotypes seven 'poropuntiin' species, namely Cosmochilus harmandi, Cyclocheilichthys apogon, Hypsibarbus malcomi, H. wetmorei, Mystacoleucus...

The chromosomes of the chevron snakehead fish (Channa striata) from Khon Kaen and Mahasarakam provinces, Northeast Thailand, were investigated by using conventional staining Ag-NOR techniques. diploid chromosome number all 2 populations was 2n=42, with karyotype composed 6 metacentric, acrocentric 34 telocentric chromosomes, NF=50, without heteromorphic sex chromosomes. This is a new report in C. striata. located on region adjacent to centromere pair 14. Here we first demonstrated bands...

The first chromosome analysis and nucleolar organizer region (NOR) pattern of the humpback cardinalfish (Fibramia lateralis) were studied. Samples from 10 male female fish collected Andaman Sea Gulf Thailand. Mitotic preparations prepared directly kidney tissues. Conventional Ag-NOR staining techniques applied to stain chromosomes. results showed that diploid number F. lateralis was 2n=46, fundamental numbers (NF) 54 in both sexes. karyotype consisted 8 large acrocentric, 12 telocentric, 24...

The multicolor banding (MCB/mBAND) technique provides a unique opportunity to characterize intrachromosomal rearrangements and determine chromosomal breakpoints. Until recently, MCB probes have only been available for human some murine chromosomes. Generation of chromosomes other species, useful required in many cytogenetics research fields, was limited by technical difficulties. are established chromosome microdissection followed whole genomic DNA amplification. However, unambiguous...

In the present study, karyotype and chromosomal characteristics of 9 species Bagridae fish family were investigated using conventional Giemsa staining as well dual-color fluorescence in situ hybridization to detect 18S 5S rDNA sites.In addition describing several catfishes, we established molecular cytogenetic techniques study this group.The contained a diploid number, varying from 50 (Pseudomystus siamensis) 62 (Hemibagrus wyckii), while none heteromorphic sex chromosomes.18S sites detected...

The chromosomal homologies of human (Homo sapiens = HSA) and silvered leaf monkey (Trachypithecus cristatus TCR) have been previously studied by classical chromosome staining fluorescence in situ hybridization (FISH) applying chromosome-specific DNA probes all chromosomes the 1980s 1990s, respectively. However, as resolution these techniques is limited we used multicolor banding (MCB) at an ~250-band level, other selected to establish a detailed map TCR. Therefore it was possible precisely...

This study examined for the first time chromosomal characteristics of nucleolar organizer regions (NORs) and karyological analysis pink anemonefish, Amphiprion perideraion (Bleeker 1955) from Andaman Sea, Phuket Province, Thailand. Kidney cell samples were taken five male female fish. Mitotic chromosome preparations conducted using a standard squash technique as well directly kidney cells. Metaphase spreads performed on microscopic slides then air-dried. Conventional Ag-NOR banding...

The catfish family Siluridae contains 107 described species distributed in Asia, but with some Europe. In this study, karyotypes and other chromosomal characteristics of 15 from eight genera were examined using conventional molecular cytogenetic protocols. Our results showed the diploid number (2n) to be highly divergent among species, ranging 2n = 40 92, modal frequency comprising 56 64 chromosomes. Accordingly, ratio uni- bi-armed chromosomes is also variable, thus suggesting extensive...

The Cyprinidae family is a highly diversified but demonstrably monophyletic lineage of cypriniform fishes.Among them, the genus Osteochilus contains 35 recognized valid species distributed from India, throughout Myanmar, Laos, Thailand, Malaysia, Indonesian archipelago to southern China.In this study, karyotypes and other chromosomal characteristics five occurring in namely O. lini, melanopleura, microcephalus, vittatus waandersii were examined using conventional molecular cytogenetic...

A new natural autotetraploid and the chromosomal characteristics of dwarf snakehead fish (Channa gachua) from Kalasin, Nong Khai, Bua Lam Phu Udon Thani Provinces (four populations) in northeast Thailand were studied. Kidney cell samples taken 12 male female fish. Mitotic chromosome preparations conducted using a blood culture technique as well directly kidney cells. Conventional Ag-NOR staining techniques applied to stain chromosomes. The results showed that number C. gachua was 4n = 104,...

The first chromosomal characteristic of F1 hybrid catfish from Thailand was studied. Kidney cell samples were taken four male and female fish. mitotic chromosome preparations done directly kidney cells. Conventional staining technique applied to stain the chromosomes. results showed that diploid number Mekong giant (Pangasianodon gigas)×striped (P. hypophthalmus) spot pangasius (Pangasius larnaudii)×P. hypophthalmus 2n=60, fundamental (NF) 108 in both males females. chromosomes presences...

Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent situ hybridization (FISH) techniques. Chromosomes directly prepared from kidney tissues stained using conventional Ag-NOR banding Microsatellite d(CA) 15 d(CGG) 10 probes hybridized to chromosomes three cyprinids. The...

A first karyotype analysis and chromosomal characteristic of nucleolar organizer regions (NORs) the common sheathfish, Micronema apogon (Bleeker 1985), from Chao Praya, Chi, Song karm basins (3 populations), Thailand, were studied. Kidney cell samples taken 10 male female fish. The mitotic chromosome preparations conducted by directly kidney, spleen and/or gill cells. Conventional Ag-NOR staining techniques applied to stain chromosomes. results showed that diploid chromosomes number M. was...