A5030291084- Photosynthetic Processes and Mechanisms
- Leptospirosis research and findings
- Veterinary medicine and infectious diseases
- Helicobacter pylori-related gastroenterology studies
- Microbial Community Ecology and Physiology
- Viral Infections and Vectors
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Plant Stress Responses and Tolerance
- Protist diversity and phylogeny
- Aquatic Ecosystems and Phytoplankton Dynamics
- Protein Structure and Dynamics
- Clostridium difficile and Clostridium perfringens research
- Biocrusts and Microbial Ecology
- Nonmelanoma Skin Cancer Studies
- Microbial Fuel Cells and Bioremediation
- Humic Substances and Bio-Organic Studies
- Light effects on plants
- Plant Micronutrient Interactions and Effects
- Antibiotic Resistance in Bacteria
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Bacterial biofilms and quorum sensing
- COVID-19 Clinical Research Studies
- Diffusion and Search Dynamics
Universidad de Zaragoza
2011-2024
Instituto de Investigación Sanitaria Aragón
2018-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2021-2024
Instituto de Biocomputación y Física de Sistemas Complejos
2018-2024
Centre for Biomedical Network Research on Rare Diseases
2024
GTx (United States)
2018
Finlay Institute
2005-2009
Hospital de Basurto
2005
Knowledge on the regulatory mechanisms controlling iron homeostasis in cyanobacteria is limited. In Anabaena sp. PCC 7120, ferric uptake regulator FurA a constitutive and essential protein whose expression induced under deprivation. Our previous analyses have shown that this acts as global transcriptional regulator, of several genes belonging to different functional categories, including schT, gene coding for TonB-dependent schizokinen transporter. present study we analysed impact...
The increasing antibiotic resistance evolved by Helicobacter pylori has alarmingly reduced the eradication rates of first-line therapies. To overcome current circulating resistome, we selected a novel potential therapeutic target in order to identify new candidate drugs for treating H. infection. We screened 1120 FDA-approved molecules that bind essential response regulator HsrA and potentially inhibit its biological function. Seven natural flavonoids were identified as binders. All these...
In the filamentous cyanobacterium Anabaena sp. PCC 7120, ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating FurA-regulatory network, number of target genes described for this essential transcription factor is limited to handful examples. article, we combine an in silico genome-wide predictive approach with experimental determinations better define regulon. Predicted FurA-binding sites were identified upstream 215...
Previous genomic analyses of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 have identified three ferric uptake regulator (Fur) homologs with low sequence identities and probably different functions in cell. FurA is a constitutive protein that shares highest homology Fur from heterotrophic bacteria appears to be essential for vitro growth. In this study, we analysed effects overexpression on phenotype investigated which observed alterations were directly operated by...
The binding affinity of NtcA towards promoter regions the microcystin gene cluster from Microcystis aeruginosa PCC 7806 has been analyzed by band-shift assay (EMSA). key nitrogen transcriptional regulator exhibits for two fragments bidirectional mcyDA promoter, as well mcyE and mcyH. presence 2-oxoglutarate increased 2.5 fold mcyA region. effect peaked at 0.8 mM, a physiological concentration this compound under nitrogen-limiting conditions. results suggest that level, signal C to N balance...
In the nitrogen-fixing heterocyst-forming cyanobacterium Anabaena sp. PCC 7120, ferric uptake regulator FurA plays a global regulatory role. Failures to eliminate wild-type copies of furA gene from polyploid genome suggest essential functions. present study, we developed selectively regulated expression system by replacement promoter in chromosomes with Co2+/Zn2+ inducible coaT Synechocystis 6803. By removing Co2+ and Zn2+ medium shutting off expression, showed that was absolutely required...
The transcriptional repressor Fur (ferric uptake regulator) is one of the most important switches regulating prokaryotic iron metabolism. Cyanobacterial FurA binds heme in micromolar concentration range and this interaction negatively affects its vitro DNA binding ability a concentration-dependent manner. Using site-directed mutagenesis along with difference absorption UV-visible, circular dichroism electronic paramagnetic resonance spectroscopies, we further analyse nature FurA. Our data...
Summary Iron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator ( FUR ) superfamily in cyanobacterium A nabaena sp. PCC 7120. Although link between iron metabolism oxidative stress management well documented, little known about connection response cyanobacteria. Zinc Z ur, also named FurB . When overexpressed E scherichia coli , ur improved cell survival during stress. In order to investigate possible correlation zur deletion...
Antibiotic resistance is a major cause of the increasing failures in current eradication therapies against Helicobacter pylori. In this scenario, repurposing drugs could be valuable strategy to fast-track novel antimicrobial agents. present study, we analyzed inhibitory capability 1,4-dihydropyridine (DHP) antihypertensive on essential function H. pylori response regulator HsrA and investigated both vitro activities vivo efficacy DHP treatments Six different commercially available highly...
The normal nasal lymphatic drainage runs via the facial vessels into neck nodes of levels I-II and this pattern was found in our patients with sinonasal tumors. We only one true-positive sentinel lymph node (SLN). Our pathological material very limited so further investigations are required before any valid conclusions can be drawn concerning clinical value SLNs Objectives. To detect collection route fossae paranasal sinuses to identify first echelon tumor metastatic cascade order prevent...
In Anabaena sp. PCC 7120, FurA is a global transcriptional regulator whose expression strongly induced by NtcA in proheterocysts and remains stably expressed mature heterocysts. the present study, overexpression of furA partially suppressed heterocyst differentiation impairing morphogenesis at an early stage. Recombinant purified specifically bound vitro to promoter regions ntcA , while quantitative RT‐PCR analyses indicated that affected transient increase occurs shortly after nitrogen...
The interplay between Fur (ferric uptake regulator) proteins and small, non-coding RNAs has been described as a key regulatory loop in several bacteria. In the filamentous cyanobacterium Anabaena sp. PCC 7120, large dicistronic transcript encoding putative membrane protein Alr1690 an α-furA RNA is involved modulation of global regulator FurA. this work we report existence three novel antisense cyanobacteria show that cis conserved very different genomic contexts, namely unicellular...
is considered the most prevalent bacterial pathogen in humans. The increasing antibiotic resistance evolved by this microorganism has raised alarm bells worldwide due to significant reduction eradication rates of traditional standard therapies. A major challenge crisis identification novel microbial targets whose inhibitors can overcome currently circulating resistome. In present study, we have validated use essential response regulator ArsR as a and promising therapeutic target against
The increasing occurrence of multidrug-resistant strains the gastric carcinogenic bacterium