A5070306959- Microfluidic and Bio-sensing Technologies
- Sepsis Diagnosis and Treatment
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Cell Image Analysis Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inhalation and Respiratory Drug Delivery
- Hemodynamic Monitoring and Therapy
- SARS-CoV-2 and COVID-19 Research
- Particle Dynamics in Fluid Flows
- Digital Holography and Microscopy
- Pleural and Pulmonary Diseases
- Lattice Boltzmann Simulation Studies
- Nosocomial Infections in ICU
- Emergency and Acute Care Studies
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Electrohydrodynamics and Fluid Dynamics
- Occupational and environmental lung diseases
- Machine Learning in Healthcare
- Image Processing Techniques and Applications
- Single-cell and spatial transcriptomics
Cytovale (United States)
2013-2024
Our Lady of the Lake Regional Medical Center
2024
Louisiana State University
2021
Drexel University
2018
University of California, Los Angeles
2009-2016
California NanoSystems Institute
2010-2015
The University of Texas Southwestern Medical Center
2014
Nanosys (United States)
2013
University of California System
2012
Cell state is often assayed through measurement of biochemical and biophysical markers. Although markers have been widely used, intrinsic markers, such as the ability to mechanically deform under a load, are advantageous in that they do not require costly labeling or sample preparation. However, current techniques assay cell mechanical properties had limited adoption clinical biology research applications. Here, we demonstrate an automated microfluidic technology capable probing single-cell...
Rapid and accurate differentiation of cell types within a heterogeneous solution is challenging but important task for various applications in biological research medicine. Flow cytometry the gold standard analysis regularly used blood (i.e., complete counts). cytometry, however, lacks sufficient throughput to analyze rare cells or other dilute solutions reasonable time period because it an inherently serial process. In this study, we exploit inertial effects label- sheath-free parallel flow...
Single-cell biophysical properties were used for diagnosing malignant pleural effusions from patients.
Abstract A general strategy for controlling particle movement across streams would enable new capabilities in single‐cell analysis, solid‐phase reaction control, and biophysics research. Transferring cells is difficult to achieve a well‐controlled manner, since it requires precise control of fluid flow along with external force fields or precisely manufactured mechanical structures. Herein introduced transfer based on passive inertial lift forces shifts the distribution these channels...
Reorganization of cytoskeletal networks, condensation and decondensation chromatin, other whole cell structural changes often accompany in state can reflect underlying disease processes. As such, the observable mechanical properties, or mechanophenotype, which is closely linked to intracellular architecture, be a useful label-free biomarker disease. In order make use this biomarker, tool measure properties should accurately characterize clinical specimens that consist heterogeneous...
Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered difficulty isolation quantification due to their size the complexity of biological samples. Conventional approaches improved require specialized equipment extensive sample preparation time. Therefore, detection methods exosomes will benefit clinical studies. Here, we report a microfluidic platform for inline exosome...
As the microenvironment of a cell changes, associated mechanical cues may lead to changes in biochemical signaling and inherently processes such as mitosis. Here we explore effects confined environments on cellular responses during Previously, confinement have been difficult optically observe three-dimensional vivo systems. To address this challenge, present novel microfluidic perfusion culture system that allows controllable variation level single axis allowing observation growth division...
Abstract In this report, we present multiparameter deformability cytometry (m-DC), in which explore a large set of parameters describing the physical phenotypes pluripotent cells and their derivatives. m-DC utilizes microfluidic inertial focusing hydrodynamic stretching single conjunction with high-speed video recording to realize high-throughput characterization over 20 different cell motion morphology-derived parameters. Parameters extracted from videos include size, deformability,...
Abstract We introduce a label-free method to rapidly phenotype and classify cells purely based on physical properties. extract 15 biophysical parameters from as they deform in microfluidic stretching flow field via high-speed microscopy apply machine-learning approaches discriminate different cell types states. When employing the full dimensional dataset, technique robustly classifies individual their pluripotency, with accuracy above 95%. Rheological morphological properties of while...
Sepsis must be diagnosed quickly to avoid morbidity and mortality. However, the clinical manifestations of sepsis are highly variable emergency department (ED) clinicians often make rapid, impactful decisions before laboratory results known. We previously developed a technique that allows measurement biophysical properties white blood cells as they stretched through microfluidic channel. In this study we describe validate resultant output model score-the IntelliSep Index (ISI)-that aids in...
OBJECTIVES: To assess the in vitro IntelliSep test, a microfluidic assay that quantifies state of immune activation by evaluating biophysical properties leukocytes, as rapid diagnostic for sepsis. DESIGN: Prospective cohort study. SETTING: Five emergency departments (EDs) Louisiana, Missouri, North Carolina, and Washington. PATIENTS: Adult patients presenting to ED with signs (two four Systemic Inflammatory Response Syndrome criteria, where one must be temperature or WBC count) suspicion...
Cells suspended in bodily fluids are routinely analyzed by cytopathologists as a means of diagnosing malignancies and other diseases. The physical morphological properties these cells evaluated making diagnostic decisions, which often requires manual concentration, staining, washing procedures to extract information about intracellular architecture. need manually prepare slides for analysis cytopathologist is labor-intensive process, ripe additional automation reduce costs but also...
Sepsis, the leading cause of mortality in hospitals, currently lacks effective early diagnostics. A new cellular host response test, IntelliSep may provide an indicator immune dysregulation characterizing sepsis. The objective this study was to examine correlation between measurements performed using test and biological markers processes associated with Phorbol myristate acetate (PMA), agonist neutrophils known induce neutrophil extracellular trap (NET) formation, added whole blood healthy...
Sepsis is a common cause of morbidity and mortality. A reliable, rapid, early indicator can help improve efficiency care outcomes. To assess the IntelliSep test, novel in vitro diagnostic that quantifies state immune activation by measuring biophysical properties leukocytes, as rapid for sepsis measure severity illness, defined Sequential Organ Failure Assessment Acute Physiology Chronic Health Evaluation-II scores need hospitalization.Adult patients presenting to two emergency departments...
The study of flow and particle dynamics in microfluidic cross-slot channels is high relevance for lab-on-a-chip applications. In this work, we investigate the a rigid spherical junction channel height-to-width ratio 0.6 at Reynolds number 120 which steady vortex exists area. Using an in-house immersed-boundary-lattice-Boltzmann code, analyse effect entry position size on trajectory shape particle. We find that depend strongly its lateral weakly vertical position; particles enter close to...
In this paper, we demonstrate a plasma misting system capable of inactivating Escherichia coli O157:H7 (E.coli) in 4 ft <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> (approximately 100 L) volume with potential for future use decontaminating fresh produce. Water droplets, an average diameter 5 μm, are generated via ultrasonic nebulizers and carried by compressed air. These micrometer-sized droplets flowthrough set three cylindrical,...
Recent advances in imaging technology for biomedicine, including high-speed microscopy, automated and flow cytometry are poised to have a large impact on clinical diagnostics, drug discovery, biological research. Enhanced acquisition speed, resolution, automation of sample handling enabling researchers probe phenomena at an increasing rate achieve intuitive image-based results. However, the rich image sets produced by these tools massive, possessing potentially millions frames with...
Abstract Lung cancers are documented to have remarkable intratumoral genetic heterogeneity. However, little is known about the heterogeneity of biophysical properties, such as cell motility, and its relationship early disease pathogenesis micrometastatic dissemination. In this study, we identified selected a subpopulation highly migratory premalignant airway epithelial cells that were observed migrate through microscale constrictions at up 100-fold rate unselected immortalized lines. This...
Abstract Objectives Sepsis is one of the most common, costly, and misdiagnosed conditions in U.S. emergency departments (EDs). ED providers often treat on nonspecific signs, subjective suspicion, or presumption infection, resulting over‐ undertreatment. An increased understanding host response has opened a new direction for sepsis diagnostics. The IntelliSep test Food Drug Administration–cleared cellular diagnostic that could help distinguish settings. Our objective was to evaluate potential...
Imaging flow cytometry uses high-speed flows and a camera to capture morphological features of hundreds thousands cells per second. These can be useful isolate sub-populations for life science research diagnostics. Our experimental setup utilizes high speed 208×32 resolution CMOS camera, operating at over 140,000 frames second (FPS). In each frame, the analysis routine detects presence an object, performs morphology measurements. Real-time cell sorting requires latency under 10 ms in...
Objective Assess the IntelliSep Index (ISI) for risk stratification of patients presenting to Emergency Department (ED) with respiratory symptoms suspected COVID-19 during pandemic. Methods An observational single-center study prospective cohort ED early pandemic and a CBC drawn within 4.5 hours initial vital signs. A sample this blood was aliquoted performance ISI, were followed clinical outcomes. The required no patient-centered activity beyond standard care treating clinicians unaware...
Abstract Sepsis must be diagnosed quickly to avoid morbidity and mortality. However, the clinical manifestations of sepsis are highly variable emergency department (ED) clinicians often make rapid, impactful decisions before laboratory results known. We previously developed a technique that allows measurement biophysical properties white blood cells as they stretched through microfluidic channel. In this study we describe validate resultant output model score – IntelliSep Index (ISI) aids in...