A5040607121- Gut microbiota and health
- Genetic diversity and population structure
- Species Distribution and Climate Change
- Wildlife Ecology and Conservation
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Clostridium difficile and Clostridium perfringens research
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Genetic and phenotypic traits in livestock
- Primate Behavior and Ecology
- Veterinary medicine and infectious diseases
- Toxin Mechanisms and Immunotoxins
- Fish Ecology and Management Studies
- Diet and metabolism studies
- Ginger and Zingiberaceae research
- Cancer-related molecular mechanisms research
- Identification and Quantification in Food
- Physiological and biochemical adaptations
- Tryptophan and brain disorders
- Synthesis and Biological Evaluation
- Coordination Chemistry and Organometallics
- Soil and Water Nutrient Dynamics
Nanjing University of Chinese Medicine
2023-2025
Nanjing Normal University
2012-2024
Lishui University
2024
University of Nebraska at Omaha
2015-2021
University of Nebraska–Lincoln
1991-2021
Institute of Zoology
2009-2019
Chinese Academy of Sciences
2007-2019
South China Agricultural University
2010-2015
The giant panda genome codes for all necessary enzymes associated with a carnivorous digestive system but lacks genes needed to digest cellulose, the principal component of their bamboo diet. It has been posited that this iconic species must therefore possess microbial symbionts capable metabolizing these have remained undetected. Here we examined 5,522 prokaryotic ribosomal RNA gene sequences in wild and captive fecal samples. We found lower richness microbiome than mammalian microbiomes...
The giant panda (Ailuropoda melanoleuca) is currently threatened by habitat loss, fragmentation, and human persecution. Its dietary specialization, isolation, reproductive constraints have led to a perception that this species at an "evolutionary dead end," destined for deterministic extinction in the modern world. Here we examine comprehensive investigation of its genetic diversity, population structure, demographic history across geographic range. We present analysis 655 base pairs...
Wild giant pandas use different parts of bamboo (shoots, leaves and stems) species at times the year. Their usage can be classified temporally into a distinct leaf stage, shoot stage transition stage. An association between this pattern variation in panda gut microbiome remains unknown. Here, we found associations using metagenomic approach nutritional analyses whereby diversity microbial community stages was significantly different. Functional analysis showed that bacteria over-represented...
Abstract Understanding population history and genetic structure are key drivers of ecological research. Here, we studied two highly fragmented isolated populations (Xiaoxiangling Daxiangling) giant pandas ( Ailuropoda melanoleuca ) at the extreme southwestern edge their distribution. This area also contains Dadu River, national road 108 various human infrastructure development, providing an ideal region in which can identify effects different barriers on animal movements. We used partial...
Gut microbes can enhance the ability of hosts to consume secondary plant compounds and, therefore, expand dietary niche breadth mammalian herbivores. The giant and red pandas are bamboo-eating specialists within order Carnivora. Bamboo contains abundant metabolites (e.g., cyanide-containing compounds). However, Carnivora species, including panda, have deficient levels rhodanese (one essential cyanide detoxification enzymes) in their tissues compared with same Here, we make a comparative...
An increasing number of studies have shown that warming also influences the animal gut microbiome (altering community structure and decreasing its diversity), which might further impact host fitness. Here, based on an analysis stomach (the entire intestine: from anterior intestine to cloaca) in laboratory larva giant salamanders ( Andrias davidianus ) under different living water temperatures (5, 15, 25°C) at two sample time points (80 330 days after acclimation), we investigated potential...
The giant panda ( Ailuropoda melanoleuca ) was taken to the brink of extinction in 1980s through a combination deforestation, large‐scale loss bamboo core its range, poaching, and zoo collection, causing over 1000 deaths from 1950s. It thought that drastic population decline likely impose severe impact on viability. Here, based temporal genotyping individuals, we show this rapid did not significantly reduce overall effective size genetic variation species, or two focal populations (Minshan...
Carnivorous members of the Carnivora reside at apex food chains and consume meat-only diets, rich in purine, fats protein. Here, we aimed to identify potential adaptive evolutionary signatures compatible with high purine fat metabolism based on analysis host genomes symbiotic gut microbial metagenomes. We found that microbiomes carnivorous (e.g., Felidae, Canidae) clustered same clade, other clades comprised omnivorous herbivorous badgers, bears pandas). The relative proportions genes...
Since 2005, 12 captive pandas have been translocated into the wild. Of these, three were killed by conditional pathogen infections after release. Given that gut microbiome is important to host health and environmental adaptation, increasing “wildness” of microbiomes might improve translocation success. Here, we analyzed long-term monitoring data (16S MISEQ about 700 samples) two wild giant panda populations (Xiaoxiangling Minshan Mountains populations), five GPS-collared (TPs), their living...
The antibiotic pollution has become an emerging environmental problem worldwide, but the ecological outcomes remain to be elucidated, especially very little is known about interactions between antibiotics and different elements. In this study, long-term influences of three representative antibiotics, i.e., tetracycline, erythromycin, sulfamethoxazole, were investigated focusing on a simplified artificial freshwater system composed amphibian tadpoles, gut bacterial fungi communities, water...
Abstract The majority of human infectious diseases originate from mammals and are inherently zoonotic. Non-human primates (NHPs) not only carriers many zoonotic pathogens, but also the best intermediary for virus shift harmless to harmful due their similar phylogenetic relationship with humans. Knowledge NHP viral composition its underlying information can therefore provide an assessment risk cross-species transmission spillover diseases. However, studies that have successfully eliminated...
Truncation is a core issue in the multivariate statistics and distribution theory. Concurrently, bivariate normal (BND) holds critical significance ℝ2. Despite pivotal importance of truncated BNDs biomedical environmental sciences, challenge parameter estimation for this on non-regular domains, including rectangle, remains inadequately tackled. This paper introduces novel normalized expectation–maximization (N-EM) algorithm to address issue, which can be achieved by innovatively partitioning...
Abstract: In conservation biology, understanding the causes of endangerment is a key step to devising effective strategies. We used molecular evidence (coalescent simulations population changes from microsatellite data) and historical information (habitat human changes) investigate how most‐isolated populations giant pandas ( Ailuropoda melanoleuca ) in Xiaoxiangling Mountains became highly endangered. These experienced strong, recent demographic reduction (60‐fold), starting approximately...
Summary The rise in infections by antibiotic‐resistant bacteria poses a serious public health problem worldwide. gut microbiome of animals is reservoir for antibiotic resistance genes (ARGs). However, the correlation between wild and ARGs remains controversial. Here, based on metagenomes giant pandas (including three populations from Qinling, Qionglai Xiaoxiangling Mountains, two major captive Yaan Chengdu), we investigated potential constitution composition across different geographic...
There are many examples of symbiotic and reciprocal relationships in ecological systems; animal gut microbiome-host interactions one such kind bidirectional complex relationship. Here, we utilized several approaches (16S rRNA gene sequencing, metagenomics, transcriptomics) to explore potential accompanying the development gastrointestinal complexity a dietary shift from metamorphosis maturity ornamented pygmy frogs (Microhyla fissipes). We identified possible coevolution between particular...
In this controlled study, we collected longitudinal fecal samples from 16 male and female marmoset monkeys for 2 weeks prior to 8 after pairing in male-female dyads. We report the first time that undergo significant changes gut microbiome following these are sex-biased; i.e., females transmit more microbes their social partners than males do. Marmosets exhibit pair bonding behavior such as spatial proximity, physical contact, grooming, sex biases behavioral patterns may contribute observed...
The gut microbiomes of non-human primates have received a great deal attention due to their close relationship humans. In recent years, these studies mainly focused on the microbiome wild primates, which will be helpful understanding evolution and (e.g., plasticity diet flexibility). However, there is still lack basic information from populations. Here, we investigated microbial composition (16S rRNA gene) function (metagenome metagenome-assembled genomes (MAGs)) Yunnan snub-nosed monkey...