A5080247698- Toxic Organic Pollutants Impact
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Pharmacogenetics and Drug Metabolism
- COVID-19 epidemiological studies
- Effects and risks of endocrine disrupting chemicals
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Air Quality and Health Impacts
- Analytical chemistry methods development
- Immune Response and Inflammation
- Pesticide Residue Analysis and Safety
- Computational Drug Discovery Methods
- Analytical Chemistry and Chromatography
- Mercury impact and mitigation studies
- Atmospheric chemistry and aerosols
- Carcinogens and Genotoxicity Assessment
- Parvovirus B19 Infection Studies
- T-cell and Retrovirus Studies
- Viral Infections and Vectors
- Sulfur-Based Synthesis Techniques
- Drug Transport and Resistance Mechanisms
- COVID-19 impact on air quality
- Pharmacological Effects and Assays
- Dermatological and COVID-19 studies
Osaka Prefectural Institute of Public Health
2013-2024
National Institute of Infectious Diseases
1988-2023
Vanderbilt University
2016
Osaka Prefecture University
2016
Konkuk University
2016
Nakamura Gakuen University
2016
Showa Pharmaceutical University
2016
Kanazawa University
1987-2015
University of Occupational and Environmental Health Japan
1996-1997
Haradoi Hospital
1997
An outbreak of coronavirus disease 2019 among passengers and crew on a cruise ship led to quarantine approximately 3,700 that began February 3, 2020, lasted for nearly 4 weeks at the Port Yokohama, Japan (1).By 9, 20 cases had occurred ship's members.By end quarantine, 700 COVID-19 been laboratory-confirmed crew.This report describes findings from initial phase investigation into members during 4-12, 2020.On 1, case was identified in passenger who developed symptoms January 23 disembarked...
During a COVID-19 outbreak on the Diamond Princess cruise ship we sampled environmental surfaces after passengers and crew vacated cabins. SARS-CoV-2 RNA was detected in 58 of 601 samples (10%) from case cabins 1-17 days were but not noncase There no difference detection proportion between symptomatic (15%, 28/189; cycle quantification [Cq], 29.79-38.86) asymptomatic cases (21%, 28/131; Cq, 26.21-38.99). No virus isolated any samples. Transmission risk patients may be similar could involved...
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Abstract A T cell line specific to human type II collagen (CII) was selected and propagated from DBA/1J mice immunized with CII. The cells were not reactive I or III of origin, but they cross-reactive bovine, rat, rabbit CII recognized both native heat-denatured an N-terminal three-quarters fragment CII, produced by tadpole collagenase digestion a C-terminal one-quarter showed Thy-1+, Lyt-1+, Lyt-2-, L3T4+ phenotypes characteristic helper delayed-type hypersensitive cells, determined the...
Benzotriazole ultraviolet stabilizers (BUVSs) used in consumer products are raising concerns as new pollutants the aquatic environment. We determined agonistic activities of eight BUVSs and a chemically distinct UV absorber (4-methylbenzylidinecamphor) toward human aryl hydrocarbon receptor (AhR) thyroid hormone receptors alpha beta. Although none showed ligand activity against receptors, four them (UV-P, UV-9, UV-326, UV-090) significant AhR activity. Their half-maximal effective...
Abstract We recently reported on an inflammatory arthropathy resembling rheumatoid arthritis that develops in high incidence among transgenic mice carry the env-pX region of human T cell leukemia virus type 1 genome. In effort to elucidate pathogenesis this disease, we found genes for cytokines, including IL-1 alpha, beta, IL-6, TNF-alpha, transforming growth factor-beta 1, IFN-gamma, and IL-2, as well MHC were activated joints. Serum levels beta IL-6 also elevated. Interestingly, these...
The systemic injection of an aqueous suspension cell wall or its peptidoglycan (PG)-rich sonicate derived from group A streptococcus and Lactobacillus casei induced acute joint lesions in BALB/c, DBA/1J, (BALB/c X DBA/1J)F1, C3H/He mouse strains, but not C57BL/6, DBA/2, AKR strains. Cell walls their enzymatically degraded PG fragments other bacteria as well the synthetic disaccharide dipeptide plantarum wall-derived tripeptide produced similar inflammation susceptible BALB/c mice. Acute...
We analyzed the mechanism by which accessory cells support induction of proliferation human peripheral blood T a monoclonal anti-CD3 antibody, OKT3. Cross-linking cell receptor/CD3 complex coupled to latex beads and addition IL-1 are not enough induce IL-2 production extensively depleted cells, while both culture supernatant macrophages or monoblastic line, U937 paraformaldehyde-fixed had been precultured with interferon-gamma before fixation into anti-CD3-latex did proliferation. Lack...
2,5,2',5'-Tetrachlorobiphenyl (TCB) induced type I binding spectra with cytochrome P450 (P450) 2A6 and 2A13, Ks values of 9.4 0.51 µM, respectively. However, CYP2A6 oxidized 2,5,2',5'-TCB to form 4-hydroxylated products at a much higher rate (∼1.0 minute-1) than CYP2A13 (∼0.02 based on analysis by liquid chromatography-tandem mass spectrometry. Formation 4-hydroxy-2,5,2',5'-TCB was greater that 3-hydroxy-2,5,2',5'-TCB three other hydroxylated products. Several human enzymes, including...
Summary Background A COVID-19 outbreak occurred in a cruise ship with 3711 passengers and crew 2020. This study is to test the hypothesis that environmental surfaces played important roles transmission for SARS-CoV-2 during this outbreak. Methods We sampled including air from common areas cabins which confirmed cases non-cases had stayed after they left cabins. tested samples by rt-PCR conducted viral isolation. Findings Of 601 tested, RNA was detected 58 (10%) case-cabins 1-17 days before...
SWR/J transgenic (tg) mice were generated expressing the TCR beta chain derived from an anticollagen type II (CII) arthritogenic T cell clone. The strain was selected because it is resistant to collagen-induced arthritis (CIA) and lacks V gene segment used by Expression of tg on all thymocytes peripheral lymph node cells led a more efficient anti-CII immune response, but did not confer CIA susceptibility mice. Nevertheless, this enhanced predisposition as (DBA/1 x SWR) F1 susceptible than...
Abstract The Diamond Princess (DP) cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong confirmed as COVID-19 case. We performed whole genome sequencing of SARS-CoV-2 directly from PCR-positive clinical specimens and conducted haplotype network analysis the outbreak. All tested isolates exhibited transversion at G 11083 T, suggesting that dissemination on DP originated single introduction event before started. Although further...
Naphthalene, phenanthrene, biphenyl, and their derivatives having different ethynyl, propynyl, butynyl, propargyl ether substitutions were examined for interaction with oxidation by cytochromes P450 (P450) 2A13 2A6. Spectral studies suggested that most of these chemicals interacted to induce Type I binding spectra more readily than Among the various substituted examined, 2-ethynylnaphthalene, 2-naphthalene ether, 3-ethynylphenanthrene, 4-biphenyl had larger ΔAmax/Ks values in inducing parent...
SUMMARY We studied the effect of superoxide dismutase (SOD) on murine collagen-induced arthritis (CIA), an animal model human rheumatoid (RA). Among SOD derivatives studied, only gelatin-SOD conjugate which has prolonged half life in vivo was effective to suppress development CIA. while native or gelatin carrier alone ineffective. Interestingly, pyran polymer-conjugated also a long showed no suppressive disease. No significant immune response against type II collagen (CII) found any...
1. We previously reported that flavone and flavanone interact spectrally with cytochrome P450 (P450 or CYP) 2A6 2A13 other human P450s inhibit catalytic activities of these enzymes. In this study, we studied abilities CYP1A1, 1A2, 1B1, 2A6, 2A13, 2C9 3A4 to oxidize flavanone.2. Human oxidized 6- 5-hydroxylated flavones, seven uncharacterized mono-hydroxylated five di-hydroxylated flavones. CYP2A6 was most active in forming 6-hydroxy- 5-hydroxyflavones several mono- products.3. also very...
1. 1-Chloropyrene, one of the major chlorinated polycyclic aromatic hydrocarbon contaminants, was incubated with human cytochrome P450 (P450 or CYP) enzymes including CYP1A1, 1A2, 1B1, 2A6, 2A13, 2B6, 2C9, 2D6, 2E1, 3A4 and 3A5. Catalytic differences in 1-chloropyrene oxidation by polymorphic two CYP1B1 five CYP2A13 allelic variants were also examined. 2. CYP1A1 oxidized at 6- 8-positions more actively than 3-position, while both CYP1B1.1 1B1.3 preferentially catalyzed 6-hydroxylation. 3....