Here we show the design, preparation, and characterization of a dormant singlet oxygen (1O2) photosensitizer that is activated upon its reaction with reactive species (ROS), including 1O2 itself, in what constitutes an autocatalytic process. The compound based on two segment photosensitizer–trap molecule where consists Br-substituted boron-dipyrromethene (BODIPY) dye. trap chromanol ring α-tocopherol, most potent naturally occurring lipid soluble antioxidant. Time-resolved absorption,...

A system composed of ethylcellulose (EC) nanofibers loading with [5,10,15,20-tetrakis(pentafluorophenyl)porphyrinato]zinc(II) (TPPF20-Zn) was developed as electrospun mats to contribute the inactivation resistant bacteria strains. This tetrapyrrolic macrocycle chosen for its excellent photostability and straightforward large-scale preparation, which are crucial attributes practical applications. The synthesis subsequent characterization TPPF20-Zn demonstrated scalability ability generate...

A novel biscarbazol triphenylamine end-capped dendrimeric zinc(II) porphyrin (DP 5) was synthesized by click chemistry. This compound is a cruciform dendrimer that bears nucleus of tetrapyrrolic macrocycle substituted at the meso positions four identical substituents. These are formed tetrafluorophenyl group possesses triazole unit in para position. nitrogenous heterocyclic connected to 4,4'-di(N-carbazolyl)triphenylamine means phenylenevinylene bridge, which allows conjugation between and...

Photodynamic inactivation (PDI) protocols using photoactive metallated porphyrin-doped conjugated polymer nanoparticles (CPNs) and blue light were developed to eliminate multidrug-resistant pathogens. CPNs-PDI varying particle concentrations irradiation doses tested against nine pathogenic bacterial strains including antibiotic-resistant bacteria of the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter...

Abstract In the field of wound healing treatment, development new materials is essential to cover multiple functions, such as acceleration process, prevention infection and response stimuli, especially if they present lower costs or are easier manufacture. These properties great importance prompt skin cicatrization. this work, multifunctional dressings for developed based on hydrogel materials. The produced via a straightforward environmentally friendly one‐pot photopolymerization...

Herein we report a simple fluorescence microscopy methodology that, jointly with four photosensitizers (PSs) and cell viability marker, allows monitoring of phenotypic bacterial resistance to photodynamic inactivation (PDI) treatments. The PSs, composed BODIPY dyes, were selected according their ability interact the wall photoinactivating mechanism involved (type I or type II). In first approach, heterogeneity allowing bacteria persist during PDI treatment was evaluated in...

A novel BOPHY-fullerene C60 dyad (BP-C60 ) was designed as a heavy-atom-free photosensitizer (PS) with potential uses in photodynamic treatment and reactive oxygen species (ROS)-mediated applications. BP-C60 consists of BOPHY fluorophore covalently attached to moiety through pyrrolidine ring. The core works visible-light-harvesting antenna, while the fullerene subunit elicits action. This fluorophore-fullerene cycloadduct, obtained by straightforward synthetic route, fully characterized...

Herein, we report the use of polylactic acid coated with a halogenated BODIPY photosensitizer (PS) as novel self-sterilizing, low-cost, and eco-friendly material activated visible light. In this article, polymeric surfaces were 3D-printed treated PS using three simple methodologies: spin coating, aerosolization, brush dispersion. Our studies showed that matrix remains unaffected upon addition PS, observed by dynamic mechanical analysis, Fourier transform infrared, scanning electron...

Methicillin-resistant<italic>Staphylococcus aureus</italic>(MRSA) biofilms eradicated by linezolid.

The spreading of different infections can occur through direct contact with glass surfaces in commonly used areas. Incorporating the use alternative therapies these materials seems essential to reduce and also avoid bacterial resistance. In this work, capability kill microbes coated two electroactive metalated phthalocyanines (ZnPc-EDOT CuPc-EDOT) is assessed. results show that both are capable producing reactive oxygen species; however, polymer Zn(II) (ZnPc-PEDOT) has a singlet quantum...

The increase in the antibiotic resistance of bacteria is a serious threat to public health. Photodynamic inactivation (PDI) micro-organisms reliable antimicrobial therapy treat broad spectrum complex infections. development new photosensitizers with suitable properties key factor consider optimization this therapy. In sense, four corroles were designed study how number cationic centers can influence efficacy antibacterial photodynamic treatments. First, 5,10,15-Tris(pentafluorophenyl)corrole...

Conjugated polymers (CPs) are known to generate radical reactive oxygen species (ROS) such as hydrogen peroxide through photoinduced charge transfer processes involving water. In this work, ability radicals is utilized here use conducting polymer nanoparticles (CPNs) photoinitiator systems (PIS) for the polymerization of acrylic monomers in aqueous media. The CPNs do not require co-initiators and also act cross-linkers. with different light-absorption properties effectively initiate...

The formation of reactive oxygen species (ROS) induced by bactericidal antibiotics has been associated with a common, nonspecific mechanism cellular death. Herein, we report real-time single-cell fluorescence studies on Escherichia coli stained fluorogenic probe for lipid peroxyl radicals showing the generation this form ROS when exposed to minimum inhibitory concentration (MIC) and 10× MIC fluoroquinolone antibiotic ciprofloxacin (3 30 μM, respectively). Single-cell intensity–time...

Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens with multi-resistant to antimicrobial agents (AA). AA provoke several changes inside bacteria, which cannot be solely explained by main mechanisms action reported. The role oxidative stress in bacteria exposed bacteriostatic has not been widely studied; hence, aim our work was investigate effect linezolid (LZD) on S. strains. Oxidative markers, such as superoxide dismutase (SOD) enzyme...

Abstract In this article, four novel fulleropyrrolidines derivatives were synthesized to study how the effect of polarity and positive charge distribution can influence efficacy photodynamic inactivation treatments kill bacteria. The design photosensitizers was based on DFT calculations that allowed us estimate dipolar moment molecules. Neutral compounds bearing N ‐methyl bis‐acetoxy‐ethyl (1) bis‐hydroxyethyl (2) amine starting material obtain dicationic analogs N,N ‐dimethyl...

In the context of addressing antimicrobial drug resistance in periocular infections, Tea Tree Oil (TTO) has emerged as a promising therapeutic option. This study aimed to assess efficacy TTO against bacterial strains isolated from ocular with particular focus on its ability inhibit biofilm formation. Additionally, we designed and analyzed microcapsules containing overcome certain unfavorable physicochemical properties enhance inherent biological attributes. The quality was confirmed through...

Innovative therapeutic approaches are required to battle the rise of antibiotic-resistant bacterial strains. Tapping on reactive oxygen species (ROS) generation in bacteria induced by bactericidal antibiotics, here we report a two-pronged strategy for inactivation relying synergistic combination antibiotic and newly designed dormant photosensitizers (DoPSs) that activate presence ROS. Intramolecular quenching renders DoPS inert light. ROS trapping aborts mechanism unmasking, equal...

A porphyrin-BODIPY dyad (P-BDP) was obtained through covalent bonding, featuring a two-segment design comprising light-harvesting antenna system connected to an energy acceptor unit. The absorption spectrum of P-BDP resulted from overlap the individual spectra its constituent parts, with fluorescence emission BODIPY unit experiencing significant quenching (96 %) due presence porphyrin Spectroscopic, computational, and redox investigations revealed competition between photoinduced electron...

Abstract The use of conjugated polymer nanoparticles (CP NPs) poly(9,9‐dioctylfluorene‐alt‐benzothiadiazole) and poly(9,9‐di‐ n ‐octylfluorenyl‐2,7‐diyl) as efficient photoinitiator systems (PIS) vinyl polymerization in water is reported herein. CP NPs are biocompatible, excitable with blue commercial LEDs and, unlike visible light Type II PIS, do not need co‐initiators to trigger a monomer chain reaction. photoinitiate variety acrylic monomers initiation rates comparable those observed for...