A5081121613- Biomedical Research and Pathophysiology
- Protease and Inhibitor Mechanisms
- Dupuytren's Contracture and Treatments
- Cell Adhesion Molecules Research
- Hedgehog Signaling Pathway Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Topic Modeling
- Speech and dialogue systems
- Natural Language Processing Techniques
- Connective tissue disorders research
- Pain Management and Placebo Effect
- Cellular Mechanics and Interactions
- Human-Automation Interaction and Safety
- graph theory and CDMA systems
- Tendon Structure and Treatment
- Genetic and Kidney Cyst Diseases
- Mechanical and Optical Resonators
- Lipid Membrane Structure and Behavior
- Healthcare Policy and Management
- Oral and gingival health research
- Economic Sanctions and International Relations
- Limits and Structures in Graph Theory
- Botulinum Toxin and Related Neurological Disorders
- Periodontal Regeneration and Treatments
- Defense, Military, and Policy Studies
Bridgewater State University
2023
Vanderbilt University Medical Center
2019-2021
University of Arkansas at Fayetteville
2012-2018
The use of semi-autonomous Unmanned Aerial Vehicles (UAV) to support emergency response scenarios, such as fire surveillance and search rescue, offers the potential for huge societal benefits. However, designing an effective solution in this complex domain represents a "wicked design" problem, requiring careful balance between trade-offs associated with drone autonomy versus human control, mission functionality safety, diverse needs different stakeholders. This paper focuses on situational...
ABSTRACT Clostridium histolyticum secretes collagenases, ColG and ColH, that cause extensive tissue destruction in myonecrosis. The C-terminal collagen-binding domain (CBD) of collagenase is required for insoluble collagen fibril binding subsequent collagenolysis. high-resolution crystal structures ColG-CBD (s3b) ColH-CBD (s3) are reported this paper. new X-ray structure s3 was solved at 2.0-Å resolution ( R = 17.4%; free 23.3%), while the previously determined s3b extended to 1.4 Å 17.9%;...
Clostridium histolyticum collagenases ColG and ColH are segmental enzymes that thought to be activated by Ca 2+ -triggered domain reorientation cause extensive tissue destruction. The consist of a collagenase module (s1), variable number polycystic kidney disease-like (PKD-like) domains (s2a s2b in s2 ColG) collagen-binding (s3 s3a s3b ColG). X-ray crystal structures -bound holo (1.4 Å resolution, R = 15.0%, free 19.1%) s2a (1.9 16.3%, 20.7%), as well -free apo (1.8 20.7%, 27.2%) two new...
Our recent work identified a genetic variant of the α345 hexamer collagen IV scaffold that is present in patients with glomerular basement membrane diseases, Goodpasture's disease (GP) and Alport syndrome (AS), phenocopies AS knock-in mice. To understand context this "Zurich" variant, an 8-amino acid appendage, we developed construct WT using single-chain NC1 trimer technology, which allowed us to solve crystal structure key connection module. The revealed ring 12 chloride ions at...
Collagen IV scaffold is a principal component of the basement membrane (BM), specialized extracellular matrix that essential for animal multicellularity and tissue evolution. Scaffold assembly begins with trimerization α-chains into protomers inside cell, which then are secreted undergo oligomerization outside cell. For ubiquitous composed α1- α2-chains, both intracellular stages mediated by noncollagenous domain (NC1). The association chloride-dependent, whereby chloride ions induce...
To penetrate host tissues, histotoxic clostridia secrete virulence factors including enzymes to hydrolyze extracellular matrix. Clostridium histolyticum, recently renamed as Hathewaya histolytica, produces two classes of collagenase (ColG and ColH). The high-speed AFM study showed that ColG moves unidirectionally plane collagen fibril rebundles when stalled . structural explanation the roles for tandem collagen-binding segment (CBDs) is illuminated by its calcium-bound crystal structure at...
Ever since the successful application of sequence to learning for neural machine translation systems, interest has surged in its applicability towards language generation other problem domains. Recent work investigated use these architectures modeling open-domain conversational dialogue, where it been found that although models are capable a good distributional model, dialogue coherence is still concern. Unlike translation, conversation much more one-to-many mapping from utterance response,...
This proposed article investigates the emergency management implications of a terrorist attack directly planned and executed by ISIL in United States. To do so, we operationalize Department Homeland Security’s National Preparedness Goal (NPG) to demonstrate how ISIL-directed attacks might stress national preparedness Core Capabilities. In so doing, provide proof concept, demonstrating viewing threat through an lens can help better benchmark existing activities policies against emerging threats.
Clinically viable Clostridium histolyticum collagenases, ColG and ColH, contain variable numbers of collagen‐binding domains (CBD) (s3a‐s3b in ColG; s3 ColH). These segments can be utilized to deliver therapeutics directly their site action. Since clinical outcomes depend on which binder is used, understanding the activation mechanisms are essential. To help elucidate these mechanisms, crystal structure Ca 2+ ‐bound tandem CBD, has been solved at 1.9 Å resolution. The revealed a pseudo‐two...