A5102763298- Advanced ceramic materials synthesis
- Metal and Thin Film Mechanics
- Fatigue and fracture mechanics
- Advanced Surface Polishing Techniques
- Microstructure and mechanical properties
- Aluminum Alloys Composites Properties
- Microstructure and Mechanical Properties of Steels
- High-Temperature Coating Behaviors
- X-ray Diffraction in Crystallography
- Advanced materials and composites
- Electronic Packaging and Soldering Technologies
- Mechanical Behavior of Composites
- High-Velocity Impact and Material Behavior
- Non-Destructive Testing Techniques
- Powder Metallurgy Techniques and Materials
- Hydrogen embrittlement and corrosion behaviors in metals
- Composite Material Mechanics
- Metallurgy and Material Forming
- Material Properties and Failure Mechanisms
- Copper Interconnects and Reliability
- Cardiac Valve Diseases and Treatments
- Advanced machining processes and optimization
- High Temperature Alloys and Creep
- Enzyme Production and Characterization
- Metal Alloys Wear and Properties
Nagoya University
2004-2024
Japanese Red Cross Nagoya Daini Hospital
2004-2024
Japan Institute of Leather Research
2014-2023
Toho University
2022
Toho University Omori Medical Center
2022
Tokyo University of Agriculture
2021
Chiba University
2019
Nagoya Industrial Science Research Institute
2018-2019
Meijo University
2007-2018
Kyoto University
1983-2017
The slip band formed in a grain on the material surface is preferential site for crack initiation during low strain fatigue of polycrystalline metals. forward and reverse plastic flow within modeled present study by dislocations with different signs moving two closely located layers, it assumed that their movement irreversible. Based model, monotonic buildup dislocation dipoles piled up at boundary systematically derived using theory continuously distributed dislocations. This associated...
To evaluate the function of cultured human corneal endothelial cells (HCECs) in vivo and feasibility HCEC transplantation with a collagen sheet as substitute carrier HCECs.Adult donor cornea derived from HCECs was labeled fluorescent tracker DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) seeded on sheet. The pump evaluated by measurement potential difference short-circuit current. A 6-mm sclerocorneal incision Descemetorhexis were performed rabbit eyes. brought into...
Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, optimal ECM components for organization have not previously been elucidated. Here, we focused on main component, fibrillar collagen, and analyzed effects of collagens by comparing use porcine heart-derived collagen other organ-derived generating engineered (EHT). We demonstrate that induces better contraction relaxation human induced pluripotent stem cell-derived EHT (hiPSC-EHT) hiPSC-EHT with exhibit...
p180 was originally reported as a ribosome-binding protein on the rough endoplasmic reticulum membrane, although its precise role in animal cells has not yet been elucidated. Here, we characterized new function of human microtubule-binding and -modulating protein. Overexpression mammalian induced an elongated morphology enhanced acetylated microtubules. Consistently, electron microscopic analysis clearly revealed microtubule bundles p180-overexpressing cells. Targeted depletion endogenous by...
The collagenase gene was cloned from Grimontia (Vibrio) hollisae 1706B, and its complete nucleotide sequence determined. Nucleotide sequencing showed that the open reading frame 2,301 bp in length encoded an 84-kDa protein of 767 amino acid residues. deduced contains a putative signal zinc metalloprotease consensus sequence, HEXXH motif. G. 60 59% identities to Vibrio parahaemolyticus alginolyticus collagenase, respectively. In contrast, this enzyme < 20% identity with Clostridium...
Abstract Background: Advances in percutaneous coronary intervention (PCI) using drug-eluting stents (DES) have impacted clinical practice. However, the efficacy of DES for dialysis patients still remains controversial. This study compares early and long-term outcomes artery bypass grafting (CABG) PCI with patients. Methods: A retrospective review was performed 125 treated between 2004 2007. Fifty-eight underwent CABG 67 DES. The overall death, cardiac cardiac-related event rates were...
There is a general consensus that collagen stability largely maintained by Pro and its major hydroxylated form, 4-hydroxyproline (4Hyp). However, positional difference in their stabilizing effect at the Xaa or Yaa position of collagenous Gly-Xaa-Yaa sequences has remained inconclusive. Here, we position-specifically evaluated correlation imino acid contents to denaturation temperature (Td) among various vertebrate invertebrate species, using recently developed LC–MS methodology. 4Hyp showed...
The misorientation introduced into an austenitic stainless steel (SUS 316) by plastic deformation was masured with the electron back scattering diffraction (EBSD) and X-ray (XRD) techniques. Three parameters related to miorientation distribution, i.e. grain orientation spread (GOS), average (GAM) kernel (KAM), were calculated from measurements EBSD. misorienation between neighboring measured points, KAM, larger close boundary where slip propagation difficult. of points within one is GAM. GAM...
Abstract In experimental fatigue tests, it may be necessary to identify the onset of crack initiation, which is often defined at a given (short) length. Different techniques are available estimate initiated size, one direct current potential drop technique. this paper, calibration curves reporting change as function depth have been derived by means 3D electrical finite element analyses. Driven previous observations, two different shapes considered: (i) circumferential and (ii)...
Basic fibroblast growth factor 2 (bFGF) is a potent mitogen for mesenchymal cells, and the local application of recombinant bFGF accelerates bone union defect repair. However, repeated dosing required sustained therapeutic effect as efficacy decreases rapidly following its diffusion from sites. Here, we attempted to develop collagen-based formation system using fusion protein (collagen binding-bFGF, CB-bFGF) consisting collagen-binding domain (CBD) Clostridium histolyticum collagenase. The...
Abstract Growth factor delivered with implantable biomaterials has been used to both accelerate and ensure healing of open fractures in human patients. However, a major limitation is the requirement for surgical placement. Here, we developed an injectable collagen material‐based bone formation system consisting powder fibril morphology triple helix conformation, basic fibroblast growth (bFGF) fused collagen‐binding domain (CBD) Clostridium histolyticum collagenase. The affinity CBD towards...
Abstract Collagenase products are crucial to isolate primary cells in basic research and clinical therapies, where their stability collagenolytic activity is required. However, currently standard collagenase from Clostridium histolyticum lack such stability. Previously, we produced a recombinant 74-kDa Grimontia hollisae , which spontaneously became truncated ~ 60 kDa possessed no In this study, generate G. useful as product, designed 62-kDa consisting only of the catalytic domain, exhibits...
The present paper reviews some recent developments of the measurements macrostress and microstress by diffraction using X-rays, synchrotron neutrons especially in Japan. These three methods are based on same principle crystals, have different advantages. conventional X-rays detect stress very near surface, while neutron takes interior materials. High-energy from sources penetration depth between suitable for measurement subsurface stresses. After describing fundamentals methods, covers...
The collagenase activity and the fpcol gene were examined in Flavobacterium psychrophilum isolates from cold-water disease (CWD)-affected ayu, Plecoglossus altivelis. Collagenase expression was closely related to accumulated mortality of CWD-affected ayu. RT-qPCR bacterial challenge experiments showed that F. ayu isolate WA-1 expressed more actively virulent than WA-2. amago (Oncorhynchus masou) WB-1, which possesses a pseudo-fpcol gene, not harmful Hitherto, well-studied metalloproteases...
Abstract Collagen is frequently digested using pepsin in industries to produce a triple helical collagen without the N‐ and C‐terminal telopeptides. However, kinetic analysis of this reaction difficult because several Lys residues telopeptides form covalent bonds, leading multiple substrates species, cleaves at various sites N‐terminal telopeptides, yielding different products. Here we performed digestion bovine type I with porcine pepsin. The could be monitored by SDS‐PAGE measuring...