A5007227178- Spine and Intervertebral Disc Pathology
- Scoliosis diagnosis and treatment
- Musculoskeletal synovial abnormalities and treatments
- Bone Tissue Engineering Materials
- Musculoskeletal pain and rehabilitation
- Orthopedic Surgery and Rehabilitation
- Inflammatory mediators and NSAID effects
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Nerve injury and regeneration
- Orthopaedic implants and arthroplasty
- Spinal Fractures and Fixation Techniques
- Bone health and osteoporosis research
- Neuropeptides and Animal Physiology
- Hip disorders and treatments
- Pain Mechanisms and Treatments
- Elbow and Forearm Trauma Treatment
- Peripheral Nerve Disorders
- Shoulder Injury and Treatment
- Immune Response and Inflammation
- Connective Tissue Growth Factor Research
- Tissue Engineering and Regenerative Medicine
- Bone and Dental Protein Studies
- Spinal Cord Injury Research
- Bone Metabolism and Diseases
Kitasato University
2014-2025
Chiba University Hospital
2021
Chiba University
2021
To investigate the role of macrophages as a regulator and producer nerve growth factor (NGF) in synovial tissue (ST) osteoarthritis (OA) joints, gene expression profiles several inflammatory cytokines ST, including fibroblasts, OA mice (STR/Ort) were characterized. Specifically, real-time polymerase chain reaction analysis was used to evaluate tumor necrosis factor- (TNF-) α , interleukin- (IL-) 1 β IL-6, NGF CD11b+ CD11b– cells isolated from ST murine model. The effects TNF- IL-1 IL-6 on...
Abstract We developed a new scaffold material—oriented collagen tubes (OCT)—and evaluated the potential of OCTs combined with basic fibroblast growth factor (bFGF) to repair 15 mm sciatic nerve defect in rats. The treatment groups consisted OCT adsorbed bFGF (OCT/bFGF group), phosphate‐buffered saline (PBS) (OCT/PBS and no‐treatment group (Defect group). Functional evaluation regeneration was performed using CatWalk system, histological analyses sites were also performed. In rats treated...
ABSTRACT Upregulation of inflammatory cytokines and various growth factors is a significant contributor to discogenic low back pain. The aim this study was investigate possible regulation pain‐related molecules by macrophages the role macrophage‐derived in injured intervertebral disc (IVD)s. C57BL/6J mice were used study. We characterized expression profiles genes for tumor necrosis factor (TNF)‐alpha, interleukin (IL)‐1beta, nerve (NGF), vascular endothelial (VEGF) both intact IVDs....
Chronic inflammation with aging contributes to sarcopenia. Previous studies have suggested that the accumulation of adipose tissue in skeletal muscle, referred as intermuscular (IMAT), increases age and is associated inflammation. However, mechanism governing ectopic muscle due not fully understood. Leptin, an adipocytokine derived from tissue, important mediator inflammatory processes. We examined changes leptin levels whether To evaluate we measured alterations expression cytokine genes...
Calcitonin gene-related peptide (CGRP) is a 37-amino-acid vasodilatory neuropeptide that binds to receptor activity-modifying protein 1 (RAMP1) and the calcitonin receptor-like (CLR). Clinical preclinical evidence suggests CGRP associated with hip knee joint pain; however, regulation mechanisms of CGRP/CGRP signaling in synovial tissue are not fully understood.Synovial tissues were harvested from 43 participants radiographic osteoarthritis (OA; unilateral Kellgren/Lawrence (K/L) grades 3-4)...
Recent studies have suggested that the tumor necrosis factor-α (TNF-α) pathway is a potential target for management of osteoarthritis (OA). Transforming growth factor (TGF)-β-activated kinase 1 (TAK1) essential in several cytokine-mediated cascades, including TNF-α, interleukin-1 (IL-1), and TGF-β pathways. The role TAK1 synovial tissue OA not fully understood. Using cells harvested from patients during surgery, we investigated whether inhibition suppresses production TNF-α-induced...
Abstract Growth factor delivered with implantable biomaterials has been used to both accelerate and ensure healing of open fractures in human patients. However, a major limitation is the requirement for surgical placement. Here, we developed an injectable collagen material‐based bone formation system consisting powder fibril morphology triple helix conformation, basic fibroblast growth (bFGF) fused collagen‐binding domain (CBD) Clostridium histolyticum collagenase. The affinity CBD towards...
Abstract Sarcopenia is defined as decreasing in muscle strength and mass, dynapenia maintained mass. This study elucidated the prevalence characteristics of sarcopenia evaluate elderly spinal disorders patients. 1039 patients aged ≥ 65 years were included. We measured age, grip strength, sagittal alignment parameters, low back pain (LBP) scores health-related quality life (HR-QoL) scores. Based on previous reports, categorised into normal group: NG, pre-sarcopenia PG, DG, SG. Pre-sarcopenia,...
To repair fractures with large bone defects or gaps, demineralized allogenic matrix (DBM) is often applied to the fracture site. However, studies have shown that use of DBM alone has limited efficacy for repairing fractures. In present study, we developed an powder (DBP) basic fibroblast-derived growth factor containing a polycystic kidney disease (PKD) domain and collagen-binding (CBD) from Clostridium histolyticum collagenase (ColH) investigated stimulatory effects bFGF-PKD-CBD combined...
Growth factor delivered in combination with animal-derived collagen materials has been used to accelerate bone fracture healing human patients. However, the introduction of bovine proteins into humans carries risk zoonotic and immunologic complications. Here, we developed a collagen-like polypeptide-based formation system consisting poly(Pro-Hyp-Gly)10 , which mimics triple helical conformation collagen, basic fibroblast growth (bFGF) fused polycystic kidney disease (PKD) domain...
Abstract Several nerve conduits have been investigated for their potential as alternative sources of autografts bridging neural gaps. However, autologous transplants remain the most effective repair. We examined clinically approved containing collagen and polyglycolic acid (PGA‐c) combined with collagen‐binding basic fibroblast growth factor (bFGF) a polycystic kidney disease (PKD) domain binding (CBD) (bFGF‐PKD‐CBD) in rat 15‐mm sciatic critical‐size defect model. The treatment groups were:...
Synovial membrane inflammation is the most common finding presenting during hip arthroscopy, and may play a role in pain. We sought to determine relationships between synovial cytokine levels, pain, arthroscopic findings of joint. prospectively included 33 patients who underwent surgery (34 hips). For all patients, radiographs severity pain were evaluated preoperatively. During we classified chondral injury synovitis, noted incidence labral tear its instability, sample was harvested for...
Abstract We investigated the relationship between trunk muscle mass and spinal pathologies by gender. This multicenter cross-sectional study included patients aged ≥ 30 years who visited a outpatient clinic. Trunk appendicular were measured using bioelectrical impedance analysis. The Oswestry Disability Index (ODI), visual analog scale (VAS) score for low back pain, sagittal vertical axis (SVA), EuroQol 5 Dimension (EQ5D) to evaluate pathology. association these parameters was analyzed...
Synovial membrane inflammation is the most commonly presenting finding during hip arthroscopy and may have a role in pathomechanism of osteoarthritis (OA). The aim this study was to determine relationship between synovial cytokine levels progression OA after arthroscopy.We prospectively examined 20 patients (20 hips) who underwent arthroscopic surgery. For all patients, radiographs severity pain were evaluated preoperatively. During arthroscopy, we harvested sample determined six typical...
Journal Article Bilateral carpal tunnel syndrome due to gouty tophi: conservative and surgical treatment in different hands of the same patient Get access Kenji Onuma, Onuma Department Orthopaedic Surgery, Kitasato University School Medicine, Sagamihara, Kanagawa, Japan Correspondence to: 1-15-1 Minami-ku, Kanagawa 252-0374, Japan. Tel: +81-42-7789343. Fax: +81-42-7785850. E-mail: onuma@med.kitasato-u.ac.jp Search for other works by this author on: Oxford Academic Google Scholar Hisako...
Study Design. An experimental study. Objective. To evaluate the effectiveness of freeze-dried bone allograft (FDBA) with basic fibroblast growth factor (bFGF) fused polycystic kidney disease domain (PKD) and collagen-binding (CBD) Clostridium histolyticum collagenase, for acceleration lumbar posterolateral fusion in rats. Summary Background Data. Reports indicate bFGF is an effective osteogenic potential promoting regeneration, although its efficiency decreases rapidly following diffusion...
Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistological analysis of spinal cord pain behavior in a rat neuropathic model were conducted to examine the function microglial basic fibroblast growth factor (bFGF) development pain.To investigate role bFGF microglia during allodynia following nerve ligation rats.Evidence suggests that production by glial cells is increased response peripheral injury. Although an association between astrocytes has been widely reported,...
Osteochondral allografting is a promising option for the treatment of large cartilage defects. However, because cell viability osteochondral tissues (OCTs) gradually reduces during storage at 4°C, methods maintaining fresh OCTs are needed to improve transplantation outcomes. Here, we evaluated whether supplementation preservation solution with one three different molecular weight forms hyaluronic acid (HA) improved rat long-term cold storage. The University Wisconsin (UW) 800 kDa...
Spinal sagittal malalignment due to vertebral fractures (VFs) induces low back pain (LBP) in patients with osteoporosis. This study aimed elucidate spinal prevalence based on VF number and patient characteristics individuals osteoporosis malalignment. alignment, were measured 259 was defined according the SRS-Schwab classification of adult deformity. evaluated number. In without VFs, bone mineral density, turnover markers, LBP scores health-related quality life (HRQoL) normal groups...
Abstract Introduction Subarachnoid hemorrhages of spinal origin are extremely rare during pregnancy. We present the case a patient with hemolytic anemia, elevated liver enzymes and low platelet count (the so-called HELLP syndrome), potentially life-threatening complication associated pre-eclampsia, who presented an idiopathic subarachnoid hematoma. Case presentation At 29 gestational weeks, 35-year-old Japanese woman was diagnosed syndrome based on bilateral leg paralysis, diminished...
Cell-based regenerative therapy has the potential to repair bone injuries or large defects that are recalcitrant conventional treatment methods, including drugs and surgery. Here, we developed a multilayered cell-based formation system using cells coated with fibronectin-gelatin (FN-G) nanofilms. The mesenchymal (MLMCs) were formed after two days of culture shown express higher levels BMP-2 VEGF compared monolayer cultures MCs. MLMCs used as graft material in combination fusion protein...