A5016285905- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Ophthalmology and Visual Impairment Studies
- Ocular Infections and Treatments
- Allergic Rhinitis and Sensitization
- Angiogenesis and VEGF in Cancer
- Retinal Diseases and Treatments
- Advanced Glycation End Products research
- Retinal and Optic Conditions
- Nasolacrimal Duct Obstruction Treatments
- Proteoglycans and glycosaminoglycans research
- Ocular Disorders and Treatments
- Lipid metabolism and disorders
- Ocular Diseases and Behçet’s Syndrome
- Ophthalmology and Eye Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tissue Engineering and Regenerative Medicine
- Retinal and Macular Surgery
- Ocular Oncology and Treatments
- Traumatic Ocular and Foreign Body Injuries
- Facial Rejuvenation and Surgery Techniques
- Retinal Development and Disorders
Inouye Eye Hospital
2009-2025
National HIV/AIDS/STI/TB Council
2024
Weatherford College
2022-2023
The University of Tokyo
2007-2016
Johns Hopkins University
2016
Eye Bank Association of America
2016
Keio University
2009-2016
Minamiaoyama Eye Clinic
2016
Christie's
2016
Boston Children's Hospital
1998-2015
Hypoxia-induced VEGF governs both physiological retinal vascular development and pathological neovascularization. In the current paper, mechanisms of neovascularization are compared contrasted. During neovascularization, absolute relative expression levels for VEGF164 increased to a greater degree than during Furthermore, extensive leukocyte adhesion was observed at leading edge pathological, but not physiological, When VEGF164-specific neutralizing aptamer administered, it potently...
Elevated vascular endothelial growth factor (VEGF) levels are required for ocular and tumor angiogenesis in animal models. Ischemic hypoxia is strongly correlated with increased VEGF expression these systems considered a physiologically relevant stimulus. Because ischemic often followed by reperfusion reactive oxygen intermediate (ROI) generation, we examined the potential role of ROI control gene expression. Human retinal pigment epithelial cells exposed to superoxide or hydrogen peroxide...
The objectives of this study were to characterize the differential potency two major VEGF isoforms, VEGF(120) and VEGF(164), for inducing leukocyte stasis (leukostasis) within retinal vasculature blood-retinal barrier (BRB) breakdown determine whether endogenous VEGF(164) mediates leukostasis BRB in early established diabetes.Retinal simultaneously quantified by combining concanavalin A lectin (ConA) perfusion labeling with a fluorophotometric dextran leakage assay. CD45 immunohistochemistry...
To evaluate the function of cultured human corneal endothelial cells (HCECs) in vivo and feasibility HCEC transplantation with a collagen sheet as substitute carrier HCECs.Adult donor cornea derived from HCECs was labeled fluorescent tracker DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) seeded on sheet. The pump evaluated by measurement potential difference short-circuit current. A 6-mm sclerocorneal incision Descemetorhexis were performed rabbit eyes. brought into...
<h3>Aims</h3> To evaluate objectively the meibomian gland area using newly developed software for non-invasive meibography. <h3>Methods</h3> Eighty eyelids of 42 patients without loss (meiboscore=0), 105 57 with less than one-third total (meiboscore=1), 13 11 between and two-thirds (meiboscore=2) 20 14 (meiboscore=3) were studied. Lid borders automatically determined. The evaluated distribution luminance and, by enhancing contrast reducing image noise, was discriminated. calculated ratio...
Vascular endothelial growth factor (VEGF) induces angiogenesis and vascular permeability is thought to be operative in several ocular diseases. The VEGF isoforms are highly conserved among species; however, little known about their differential biological functions adult tissue. In the current study, inflammatory potential of two prevalent isoform splice variants, VEGF(120(121)) VEGF(164(165)), was studied transparent avascular mouse cornea.Controlled-release pellets containing equimolar...
To compare central corneal thickness measurements taken with 3 pachymetry systems: Orbscan scanning-slit topography/pachymetry, Topcon SP2000P noncontact specular microscopy, and Tomey ultrasonic pachymetry.Multicenter study, Tokyo, Japan.In 216 healthy eyes of 114 subjects, topography, were used in that sequence to record thickness. In another 20 13 2 sets repeated for each assess repeatability.The mean was compatible between topography (546.9 micrometers +/- 35.4 [SD] ) (548.1 33.0...
To isolate precursors of human corneal endothelial cells (HCECs) in vitro.HCECs were subjected to a sphere-forming assay which spheres floated serum-free medium containing growth factors. promote differentiation, the isolated sphere colonies plated dishes coated with poly-L-lysine (PLL)/laminin or fetal bovine endothelium extracellular matrix. Marker expression neural and mesenchymal was examined their progenies by immunocytochemistry and/or reverse transcription-polymerase chain reaction...
To evaluate risk factors for graft failure and allograft rejection after penetrating keratoplasty (PK).We retrospectively studied clinical results of PKs in terms survival rejection-free rates. were done on 271 eyes between 1987 1997. Clinical analyzed by Kaplan-Meier's life table method the log-rank test. Relative risks adjusted probabilities each value factor compared with a specified reference value.The overall rates 10 years PK 79.3% 77.9%, respectively. Higher relative was associated...
To present the results of a national survey on bullous keratopathy (BK) in Japan.A cross-sectional was conducted for 963 eyes with BK seen between 1999 and 2001 by members Japan Cornea Society. Demographic characteristics, type surgery, complications, postoperative outcome were analyzed.BK accounted 24.2% (963 eyes) total keratoplasties performed during period. Graft clarity maintained 77.4% cases, immunologic rejection elevated intraocular pressure noted 10.8% 15.3%, respectively. Cataract...