A5101472235- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Ocular Diseases and Behçet’s Syndrome
- Retinal and Optic Conditions
- Retinal and Macular Surgery
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Ocular Oncology and Treatments
- Intraocular Surgery and Lenses
- Renin-Angiotensin System Studies
- Advanced Glycation End Products research
- Lymphoma Diagnosis and Treatment
- CNS Lymphoma Diagnosis and Treatment
- Receptor Mechanisms and Signaling
- Cerebral Venous Sinus Thrombosis
- Vascular Malformations Diagnosis and Treatment
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Systemic Lupus Erythematosus Research
- Angiogenesis and VEGF in Cancer
- Connexins and lens biology
- Galectins and Cancer Biology
- Ocular Infections and Treatments
- Corneal surgery and disorders
- Retinoids in leukemia and cellular processes
Hokkaido University
2016-2025
Creative Commons
2022
Ophthalmology Associates (United States)
2000-2020
Health Sciences University of Hokkaido
2012-2018
National Institute of Health Sciences
1981-2018
The University of Tokyo
2009-2015
Tokyo Medical and Dental University
2015
Kyoto University
2015
The Open University of Japan
2015
Keio University
2004-2013
Hypoxia-induced VEGF governs both physiological retinal vascular development and pathological neovascularization. In the current paper, mechanisms of neovascularization are compared contrasted. During neovascularization, absolute relative expression levels for VEGF164 increased to a greater degree than during Furthermore, extensive leukocyte adhesion was observed at leading edge pathological, but not physiological, When VEGF164-specific neutralizing aptamer administered, it potently...
Diabetic retinopathy is a progressive neurodegenerative disease, but the underlying mechanism still obscure. Here, we focused on oxidative stress in retina, and analysed its influence retinal neurodegeneration, using an antioxidant, lutein.C57BL/6 mice with streptozotocin-induced diabetes were constantly fed either lutein-supplemented diet or control from onset of diabetes, their metabolic data recorded. In 1-month-diabetic mice, reactive oxygen species (ROS) retina measured dihydroethidium...
The objectives of this study were to characterize the differential potency two major VEGF isoforms, VEGF(120) and VEGF(164), for inducing leukocyte stasis (leukostasis) within retinal vasculature blood-retinal barrier (BRB) breakdown determine whether endogenous VEGF(164) mediates leukostasis BRB in early established diabetes.Retinal simultaneously quantified by combining concanavalin A lectin (ConA) perfusion labeling with a fluorophotometric dextran leakage assay. CD45 immunohistochemistry...
Circulating interleukin-6 (IL-6) levels were determined using a sensitive enzyme immunoassay in adults with asthma stable condition during naturally occurring attacks and before after allergen inhalation tests. IL-6 was significantly elevated even asymptomatic asthmatic subjects (n = 17) compared normal control 17). During attacks, serum comparison those symptom-free (4 wk interval; n 8, p < 0.01). No significant difference observed obtained from the period 10). There elevation circulating...
To investigate the involvement of renin-angiotensin system (RAS) and nuclear factor (NF)-kappaB pathway with diabetes-induced retinal inflammation.Six weeks after induction diabetes, C57BL/6 mice were treated angiotensin II type 1 receptor (AT1-R) blocker (ARB) telmisartan or valsartan, AT2-R PD123319, NF-kappaB inhibitor dehydroxymethylepoxyquinomicin (DHMEQ) daily for week. Retinal mRNA protein levels RAS components examined by RT-PCR Western blot, respectively. Leukocyte adhesion to...
Background— Choroidal neovascularization (CNV) is a critical pathogenesis in age-related macular degeneration, the most common cause of blindness developed countries. The aim current study was to investigate effect lutein supplementation on development murine model laser-induced CNV together with underlying molecular mechanisms. Methods and Results— Mice were orally pretreated daily from 3 days before laser photocoagulation untill end study. index volume significantly suppressed by treatment...
Resveratrol is known as one of the antioxidant polyphenols contained in red wine and grape skin. The purpose present study was to investigate role resveratrol ocular inflammation endotoxin-induced uveitis (EIU).EIU induced male C57/B6 mice at age 6 weeks by a single intraperitoneal injection lipopolysaccharide (LPS). Animals had received oral supplementation doses 5, 50, 100, or 200 mg/kg for 5 days until LPS injection. Twenty-four hours after administration, leukocyte adhesion retinal...
Lutein has been the focus of recent study as a possible therapeutic approach for retinal diseases, but molecular mechanism its neuroprotective effect remains to be elucidated. The aim this was investigate, with use mouse endotoxin-induced uveitis (EIU) model, effects lutein against neural damage caused by inflammation.EIU induced intraperitoneal injection lipopolysaccharide (LPS). Each animal given subcutaneous or vehicle three times: concurrently and 3 hours before after LPS injection....
This paper reports a multi-throughput multi-organ-on-a-chip system formed on pneumatic pressure-driven medium circulation platform with microplate-sized format as novel type of microphysiological system. The enabled parallelized multi-organ experiments (i.e. simultaneous operation multiple culture units) and pipette-friendly liquid handling for various conventional cell experiments, including seeding, change, live/dead staining, growth analysis, gene expression analysis collected cells,...
OBJECTIVE The term “receptor-associated prorenin system” (RAPS) refers to the pathogenic mechanisms whereby binding its receptor dually activates tissue renin-angiotensin system (RAS) and RAS-independent intracellular signaling via receptor. aim of present study was define association RAPS with diabetes-induced retinal inflammation. RESEARCH DESIGN AND METHODS Long-Evans rats, C57BL/6 mice, angiotensin II type 1 (AT1-R)-deficient mice streptozotocin-induced diabetes were treated (pro)renin...
To investigate the role of reactive oxygen species (ROS) as prime initiators angiogenic response after alkali injury cornea and observe effects antioxidants in preventing angiogenesis.The corneal epithelia SOD-1-deficient mice or wild-type (WT) were removed application 0.15 N NaOH to establish animal model burn. ROS production was semiquantitatively measured by dihydroethidium (DHE) fluorescence. Angiogenesis visualized CD31 immunohistochemistry. The specific NF-κB inhibitor DHMEQ,...
AMP-activated protein kinase (AMPK) is a sensor of cellular energy status. The purpose the present study was to elucidate roles AMPK in pathogenesis diabetic retinopathy using known activators resveratrol and AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) mouse model.C57BL/6 mice with streptozotocin-induced diabetes were treated orally at 50 mg/kg for 7 days or intraperitoneally 100 24 hours before death. Retinal levels phosphorylated total AMPK, nuclear factor (NF)-κB p65,...
<h3>Objectives</h3> To identify non-major histocompatibility complex susceptible genes that might contribute to Behçet9s disease (BD). <h3>Methods</h3> We performed a genome-wide association study using DNA samples from Korean population consisting of 379 BD patients and 800 controls. A replication was in Japanese (363 272 controls). evaluate the functional implication target single nucleotide polymorphisms (SNP), gene expression levels peripheral T cells, allele-specific modulation promoter...
Vascular endothelial growth factor (VEGF) induces angiogenesis and vascular permeability is thought to be operative in several ocular diseases. The VEGF isoforms are highly conserved among species; however, little known about their differential biological functions adult tissue. In the current study, inflammatory potential of two prevalent isoform splice variants, VEGF(120(121)) VEGF(164(165)), was studied transparent avascular mouse cornea.Controlled-release pellets containing equimolar...
The interaction of advanced glycation end products (AGEs) with their receptors is hypothesized to be involved in the development diabetic retinopathy. In present study, role an AGE receptor, RAGE, was investigated retinopathy vivo.C57/BJ6 and RAGE-transgenic mice that carried human RAGE genetic DNA under control murine flk-1 promoter were made streptozocin. Three months after onset diabetes, soluble form (sRAGE) or mouse serum albumin injected intraperitoneally at 100 mug/d for 14...
OBJECTIVE—Pathogenic mechanisms underlying diabetes-induced retinal dysfunction are not fully understood. The aim of the present study was to show relationship renin-angiotensin system (RAS) with synaptic vesicle protein synaptophysin and neuronal activity in diabetic retina. RESEARCH DESIGN AND METHODS—C57BL/6 mice streptozotocin-induced diabetes were treated angiotensin II type 1 receptor (AT1R) blocker telimsartan or valsartan, function analyzed by electroretinography. Retinal production...
To investigate the retinal neural damage that occurs during inflammation and therapeutic effects of angiotensin II type 1 receptor (AT1R) blocker, telmisartan, using a model endotoxin-induced uveitis (EIU).The localization AT1R AT2R was shown by immunohistochemistry. EIU induced intraperitoneal injection lipopolysaccharide (LPS). Animals were treated with telmisartan for 2 days evaluated 24 hours later. Expression levels II, STAT3 activation inflammatory cytokines, proteins essential...
Choroidal neovascularization (CNV) is a critical pathogenesis in age-related macular degeneration, the most common cause of blindness developed countries. The aim current study was to determine involvement renin-angiotensin system (RAS) with development CNV, using human surgical samples and murine model laser-induced CNV.In CNV tissues, vascular endothelium expressed angiotensin II type 1 receptor (AT1-R), AT2-R, II. volume significantly suppressed by treatment an AT1-R blocker telmisartan,...
Diabetes is a systemic disease that causes number of metabolic and physiologic abnormalities. One the major microvascular complications diabetes diabetic retinopathy (DR), leading cause blindness in people over age 50. The mechanisms underlying development DR are not fully understood; however, extensive studies have recently implicated chronic, low-grade inflammation pathophysiology DR. During leukocytes undergo sequential adhesive interactions with endothelial cells to migrate into inflamed...