A5051064869- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Cannabis and Cannabinoid Research
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Chemical Synthesis and Analysis
- Melamine detection and toxicity
- Synthesis and Biological Evaluation
- Biopolymer Synthesis and Applications
- Research in Social Sciences
- Cancer Research and Treatments
- Identification and Quantification in Food
- Mast cells and histamine
- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Antimicrobial agents and applications
- Cancer-related gene regulation
- Advanced Chemical Sensor Technologies
- Estrogen and related hormone effects
- Soil and Water Nutrient Dynamics
- Synthesis and Characterization of Heterocyclic Compounds
- Trace Elements in Health
- Neuroblastoma Research and Treatments
- Muscle activation and electromyography studies
- Nicotinic Acetylcholine Receptors Study
University of Eastern Finland
2013-2022
Finland University
2011-2017
Karolinska Institutet
2014
Engelhardt Institute of Molecular Biology
2005-2012
National Institute of Dental and Craniofacial Research
2011
National Institutes of Health
2000-2011
Institute for Molecular Science
2002-2010
Institute for Molecular Medicine Finland
2010
Rutgers Cancer Institute of New Jersey
2009
Kuopio University Hospital
2000
We evaluate the ability of an electronic nose to discriminate prostate cancer from benign prostatic hyperplasia using urine headspace, potentially offering a clinically applicable noninvasive and rapid diagnostic method.The ChemPro® 100-eNose was used sample headspace. Its performance tested with 50 patients confirmed 24 samples 15 (15 provided preoperatively 9 3 months postoperatively) scheduled undergo robotic assisted laparoscopic radical prostatectomy or transurethral resection prostate,...
Polyamines are required for optimal growth and function of cells. Regulation their cellular homeostasis is therefore tightly controlled. The key regulatory enzyme polyamine catabolism the spermidine/spermine N 1 -acetyltransferase (SSAT). Depletion polyamines has been associated with inhibition programmed cell death. To investigate physiological SSAT, we generated a transgenic rat line overexpressing SSAT gene under control inducible mouse metallothionein I promoter. Administration zinc...
Spermidine/spermine N 1 -acetyltransferase (SSAT), the rate-controlling enzyme in interconversion of spermidine and spermine, is regulated by polyamines their analogs at many levels gene expression. Recently, SSAT pre-mRNA has been shown to undergo alternative splicing inclusion an exon that contains premature termination codons. In present study, we show alterations intracellular polyamine level resulted a change relative abundance transcripts. Addition or N-diethylated reduced amount...
We have earlier shown that alpha-methylated spermidine and spermine analogues rescue cells from polyamine depletion-induced growth inhibition maintain pancreatic integrity under severe deprivation. However, because alpha-methylspermidine can serve as a precursor of hypusine, an integral part functional eukaryotic translation initiation factor 5A required for cell proliferation, alpha, omega-bismethylspermine be converted to methylspermidine, it is not entirely clear whether the restoration...
Polyamines are essential for cell proliferation, and their levels elevated in many human tumors. The oncogene n-myc is known to potentiate polyamine metabolism. Neuroblastoma, the most frequent extracranial solid tumor children, harbors amplification of 25% cases, it associated with treatment failure poor prognosis. We evaluated several metabolic features neuroblastoma lines Kelly, IMR-32, SK-N-SH. further investigated effects glycolysis impairment metabolism these lines. A previously...
Elevated concentrations of polyamines have been found in urine patients with malignant tumors, including ovarian cancer. Previous research has suffered from poorly standardized detection methods. Our liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is capable simultaneous analysis most known polyamines. Liquid not previously used the differential diagnostics tumors postmenopausal women.In this prospective study, women (n = 71) presenting an adnexal and, as controls, genital...
We recently generated a transgenic rat model for acute pancreatitis, which was apparently caused by massive depletion of pancreatic polyamines spermidine and spermine due to inducible activation their catabolism (Alhonen, L., Parkkinen, J. J., Keinänen, T., Sinervirta, R., Herzig, K. H., Jänne, (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 8290–8295). When subjected partial hepatectomy, these animals showed striking polyamine at 24 h postoperatively with profound decrease in hepatic pools...
Impaired adipogenesis has been shown to predispose disturbed adipocyte function and development of metabolic abnormalities. Previous studies indicate that polyamines are essential in the 3T3-L1 fibroblasts. However, specific roles individual during have remained ambiguous as natural readily interconvertible inside cells. Here, we defined spermidine spermine cells by using (S')- (R')- isomers alpha-methylspermidine (S,S')-, (R,S)- (R,R')-diastereomers alpha,omega-bismethylspermine. Polyamine...
Polyamines are essential molecules supporting the structure, conformation, and function of nucleic acids proteins. We studied stereoisomers α,α′-dimethylated spermine [(R,R)-Me2Spm, (S,S)-Me2Spm, (R,S)-Me2Spm] for their ability to provoke DNA condensation protect from damage. (R,R)- (R,S)-Me2Spm displayed more efficient condensing than spermine, with significantly lower EC50 (concentration 50% compaction) values (p ≤ 0.01). However, exerted slightly duplex stabilization Me2Spm. Condensation...
The biogenic polyamines, spermine, spermidine (Spd) and putrescine (Put) are present at micro-millimolar concentrations in eukaryotic prokaryotic cells (many prokaryotes have no spermine), participating the regulation of cellular proliferation differentiation. In mammalian Put is formed exclusively from L-ornithine by ornithine decarboxylase (ODC) many potent ODC inhibitors known. bacteria, plants, fungi synthesized also agmatine, which L-arginine arginine (ADC). Here we demonstrate that...
The polyamines are ubiquitous short-chain aliphaticamines essential for the growth and proper function of normal cells.The multiple roles at molecular level not completely clarified but due to their cationic nature able bind various negatively charged structures macromolecules such as DNA, RNA, proteins phospholipids [1].Rather constant intracellular levels controlled by biosynthetic catabolic enzymes well transport in response changes cel-Mice with targeted disruption spermidine/spermine N
Metabolically stable polyamine derivatives may serve as useful surrogates for the natural polyamines in studies aimed to elucidate functions of individual polyamines. Here we studied metabolic stability α-methylspermidine, α-methylspermine, and bis-α-methylspermine, which all have been reported fulfill many putative physiological In vivo were performed with transgenic rats overexpressing spermidine/spermine N1-acetyltransferase. α-Methylspermidine effectively accumulated liver did not appear...
Acute pancreatitis is an autodigestive disease, in which the pancreatic tissue damaged by digestive enzymes produced acinar cells. Among tissues mammalian body, pancreas has highest concentration of natural polyamine, spermidine. We have found that very sensitive to acute decreases concentrations higher polyamines, spermidine and spermine. Activation polyamine catabolism transgenic rats overexpressing SSAT (spermidine/spermine-N1-acetyltransferase) leads rapid depletion these polyamines...
We have generated mouse embryonic stem cells with targeted disruption of spermidine/spermine<i>N</i> <sup>1</sup>-acetyltransferase (SSAT) gene. The did not contain any inducible SSAT activity, and the protein was present. SSAT-deficient proliferated normally appeared to maintain otherwise similar polyamine pools as wild-type cells, possible exception constantly elevated (about 30%) cellular spermidine. As expected, mutated were significantly more resistant toward growth-inhibitory action...