A5004038353- Hepatitis C virus research
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Hepatitis B Virus Studies
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- HIV Research and Treatment
- Cannabis and Cannabinoid Research
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Phosphodiesterase function and regulation
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Chemical Reactions and Isotopes
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer therapeutics and mechanisms
- Protein purification and stability
- Viral gastroenteritis research and epidemiology
- Carbohydrate Chemistry and Synthesis
- Genomics, phytochemicals, and oxidative stress
Engelhardt Institute of Molecular Biology
2004-2023
Russian Academy of Sciences
1985-2015
Karolinska Institutet
2012
Lomonosov Moscow State University
1975-1980
Fluoroquinolones are an important class of modern and efficient antibacterial drugs with a broad spectrum activity. Levofloxacin (the optically active form ofloxacin) is one the most promising fluoroquinolone drugs, its activity substantially higher than other family. Earlier, in Postovsky Institute Organic Synthesis, UB RAS, original method levofloxacin synthesis was developed, now pilot batch drug being prepared. Bacterial DNA gyrase specific target fluoroquinolones; hence, study...
A mutant T7 RNA polymerase (T7 RNAP) having two amino-acid substitutions (Y639F and S641A) is altered in its specificity towards nucleotide substrates, but not affected the of interaction with promoter terminator sequences. The enzyme gains ability to utilize dNTPs catalyze DNA synthesis from circular supercoiled plasmid DNA. can also be initiated a single stranded template using primer. Another RNAP only substitution S641A loses activity able synthesize
Abstract Hepatitis C Virus (HCV) is a major public health problem worldwide. While highly efficacious directly-acting antiviral agents have been developed in recent years, their high costs and relative inaccessibility make use limited. Here, we describe new 1-(ω-phenoxyalkyl)uracils bearing acetanilide fragment 3 position of pyrimidine ring as potential drugs against HCV. Using combination various biochemical assays vitro virus infection replication models, show that our compounds are able...
The hepatitis C virus (HCV) triggers a chronic disease that is often accompanied by spectrum of liver pathologies and metabolic alterations. oxidative stress occurs in the infected cells considered as one mechanisms HCV pathogenesis. It induced viral core NS5A proteins. already known both these proteins activate antioxidant defense system controlled Nrf2 transcription factor. Here, we show this activation mediated domain 1 protein two fragments protein. In cases, achieved through mechanisms....
A highly selective affinity labeling of T7 RNA polymerase with the o ‐formylphenyl ester GMP and [α‐ 32 P]UTP was carried out. The site located using limited cleavages hydroxylamine, bromine, N ‐chlorosuccinimide cyanogene bromide identified as Lys631 residue. Site‐directed mutagenesis synthetic oligonucleotides used to substitute by a Gly, Leu or Arg Kinetic studies purified mutant enzymes showed alterations their polymerizing activity. For Lys → Gly enzyme, anomalous template binding observed.
The polyamines, spermine (Spm) and spermidine (Spd), are important for cell growth function. Their homeostasis is strictly controlled, a key downregulator of the polyamine pool polyamine-inducible protein, antizyme 1 (OAZ1). OAZ1 inhibits uptake targets ornithine decarboxylase (ODC), rate-limiting enzyme biosynthesis, proteasomal degradation. Here we report, first time, that polyamines induce dimerization mouse recombinant full-length OAZ1, forming an (OAZ1)2-Polyamine complex. Dimerization...
Oligonucleotides of a novel type containing 2′‐ O ‐β‐ribofuranosyl‐cytidine were synthesized and further oxidized to yield T7 consensus promoters with dialdehyde groups. Both types oligonucleotides tested as templates, inhibitors, affinity reagents for RNA polymerase its mutants. All retained high towards the enzyme. Wild‐type most mutants did not react irreversibly oligonucleotides. Affinity labeling was observed only promoter‐containing group in position (+2) coding chain one tested,...
The efficient cell-mediated immune response clears cells expressing deoxyribonucleic acid (DNA) immunogens, but there are no methods to monitor this in vivo. We hypothesized that immune-mediated clearance can be monitored vivo if DNA immunogens coexpressed with reporter(s). To test this, we designed genes encoding human immunodeficiency virus 1 (HIV-1) reverse transcriptase (RT) fused via its N- or C-terminus 30-amino acid-long Gly-Ala-repeat of Epstein-Barr nuclear antigen the N-terminus...
Hepatitis C virus (HCV) triggers massive production of reactive oxygen species (ROS) and affects expression genes encoding ROS-scavenging enzymes. Multiple lines evidence show that levels ROS contribute to the development various virus-associated pathologies. However, investigation HCV redox biology so far remained in paradigm oxidative stress, whereas no attention was given identification switches among viral proteins. Here, we report one such is NS5B protein exhibits RNA-dependent RNA...
Hepatitis delta virus (HDV) is a viroid-like blood-borne human pathogen that accompanies hepatitis B infection in 5% patients. HDV has been studied for four decades; however, the knowledge on its life-cycle and pathogenesis still sparse. The studies are hampered by absence of commercially-available HDV-specific antibodies. Here, we describe set reproducible methods expression E. coli His-tagged small antigen (S-HDAg), purification, production polyclonal anti-S-HDAg antibodies rabbits. S-HDAg...
MINT‐6803713: CKII (uniprotkb:P67870) phosphorylates (MI:0217) NS5A (uniprotkb:P26662) by protein kinase assay (MI:0424)
The biogenic polyamines, spermidine (Spd) and spermine (Spm), are present at millimolar concentrations in all eukaryotic cells, where they participate the regulation of vitally important cellular functions. Polyamine analogs derivatives a traditional instrument for investigation functions enzymes their metabolism, biosynthesis antizyme—a key downregulator polyamine homeostasis. Here, we describe convenient gram-scale syntheses set C-methylated Spd. biochemical properties these compounds...