A5011502725- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Microbial bioremediation and biosurfactants
- Plant-Microbe Interactions and Immunity
- Microplastics and Plastic Pollution
- Cancer Research and Treatments
- Bacillus and Francisella bacterial research
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Biofuel production and bioconversion
- Cystic Fibrosis Research Advances
- Enzyme Catalysis and Immobilization
- Transgenic Plants and Applications
- Protein Structure and Dynamics
- GABA and Rice Research
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
University of Hohenheim
2019-2024
Institute of Food Science
2020
Abstract Background Plipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, application of this has so far been investigated in few cases, principally because yield low concentration and unknown regulation biosynthesis pathways. B. subtilis synthesizes plipastatin by non-ribosomal peptide synthetase encoded ppsABCDE operon. In study, 3NA (a non-sporulation strain) was engineered gain...
Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites applications in agriculture. Therefore, identification of productive wild-type is essential the development biopesticides. velezensis FZB42 a well-established strain biocontrol plant pathogens Here, we characterized an alternative strain, B. UTB96, that higher amounts all three major lipopeptide families, namely surfactin, fengycin, and iturin. UTB96 produces...
In a scenario of increasing alarm about food waste due to rapid urbanization, population growth and lifestyle changes, this study aims explore the valorization from retail sector as potential substrates for biotechnological production biosurfactants. With perspective increasingly contributing realization circular bioeconomy, vegetable juice, derived unsold fruits vegetables, carbon source was used produce lipopeptides such surfactin fengycin. The results shake flask cultivations revealed...
Abstract Background The production of surfactin, an extracellular accumulating lipopeptide produced by various Bacillus species, is a well-known representative microbial biosurfactant. However, only limited information available on the correlation between growth rate strain, such as B. subtilis BMV9, and surfactin production. To understand biomass formation over time production, availability glucose carbon source was considered main point. In fed-batch bioreactor processes, BMV9 used, strain...
Abstract Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B . strains JABs24 and DSM10 T , surfactin plipastatin are produced. Lipopeptide formation controlled, among others, by DegU response regulator. The activating phospho‐transfer DegS sensor kinase stimulated pleiotropic regulator DegQ, resulting in enhanced activation. 168, point mutation degQ promoter region leads to reduction gene expression. Corresponding reporter showed 14‐fold...
Abstract As a lipopeptide (LP), surfactin exhibits properties, such as emulsifying and dispersing ability, which are useful in food industry. Discovery of new LP-producing strains from sources is an important step towards possible application foods. A total 211 spore-forming, Gram-positive, catalase-positive bacterial were isolated fermented African locust beans (iru) palm oil mill effluents screening process examined for their ability to produce surfactin. This was achieved by combination...
Background: Due to their structural features, biosurfactants reveal promising physicochemical properties, making them interesting for various applications in different fields, such as the food, cosmetics, agriculture, and bioremediation sectors. In particular, bioproduction of surfactin, one most potent microbially synthesized biosurfactant molecules, is great interest. However, since wild-type productivities are comparably low, stimulatory environmental conditions have be identified...
Introduction:B. velezensis strains are of interest in agricultural applications due to their beneficial interactions with plants, notable through antimicrobial activity. The biocontrol ability two new lipopeptides-producing B. ES1-02 and EFSO2-04, against fungal phytopathogens Diaporthe spp., was evaluated compared reference QST713 FZB42. All were found be effective the plant pathogens, showing comparable antifungal activity slightly lower than Methods: Lipopeptides isoforms identified by...
Abstract Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending on applied biomass source material as well process conditions. Consequently, this results in variable composition such different sugar concentrations and the presence inhibitors formed during hydrolysis. These key obstacles commonly limit its efficient use carbon for biotechnological conversion. The gram‐negative soil bacterium Pseudomonas putida KT2440 is promising candidate future...
Bacillus velezensis UTB96 was isolated from soil based on its antifungal activity. Whole-genome sequencing of strain provided further information about secondary metabolite gene clusters. Compared to the well-known FZB42, lacks an IS3 element and a type I restriction endonuclease.
The complex regulatory network in Bacillus, known as quorum sensing, offers many opportunities to modify bacterial gene expression and hence control bioprocesses. One target regulated by this mechanism is the activity of PsrfA promoter, which engaged formation lipopeptide surfactin. It was hypothesised that deletion rapC, rapF rapH, encoding for prominent Rap-phosphatases affect activity, would enhance surfactin production. Therefore, these genes were deleted a sfp+ derivative B. subtilis...
Surfactin, a potent biosurfactant produced by
Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B. strains JABs24 and DSM10T, surfactin, plipastatin are produced. Lipopeptide formation controlled, among others, by DegU response regulator. The activating phospho-transfer DegS sensor kinase stimulated pleiotropic regulator DegQ, resulting in enhanced activation. 168, point mutation degQ promoter region leads to reduction gene expression. Corresponding reporter showed 14-fold reduced This...
Abstract Background: Plipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, application of this has so far been investigated in few cases, principally because yield low concentration and unknown regulation biosynthesis pathways. B. subtilis synthesizes plipastatin by non-ribosomal peptide synthetase encoded ppsABCDE operon. In study, 3NA (a non-sporulation strain) was engineered gain...
Abstract Background: Plipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, application of this has so far been investigated in few cases, principally because yield low concentration and unknown regulation biosynthesis pathways. B. subtilis synthesizes plipastatin by non-ribosomal peptide synthetase encoded ppsABCDE operon. In study, 3NA (a non-sporulation strain) was engineered gain...
Abstract Background Fengycin and plipastatin are potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, application of these lipopeptides has so far been investigated in a few cases, principally because their yield low concentration unknown regulation biosynthesis pathways. B. subtilis synthesizes by non-ribosomal peptide synthetase encoded ppsABCDE operon. In this study, 3NA (a non-sporulation...