A5005557027- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Transgenic Plants and Applications
- Protein Hydrolysis and Bioactive Peptides
- Microbial bioremediation and biosurfactants
- Enzyme Production and Characterization
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Horticultural and Viticultural Research
- Vibrio bacteria research studies
- Proteins in Food Systems
- Plant-derived Lignans Synthesis and Bioactivity
- Plant tissue culture and regeneration
- Biochemical and Structural Characterization
- Plant Physiology and Cultivation Studies
- Microbial metabolism and enzyme function
- Chemical synthesis and alkaloids
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Peptidase Inhibition and Analysis
- Plant Molecular Biology Research
- Cancer Research and Treatments
- Axial and Atropisomeric Chirality Synthesis
- Viral Infectious Diseases and Gene Expression in Insects
University of Hohenheim
2016-2025
Biocat
2021
University of Stuttgart
2009
(+)- or (−)-pinoresinol: that is the question. Which of two enantiomeric lignans formed during laccase-catalyzed phenol coupling (E)-coniferyl alcohol (1) depends on dirigent protein. In presence first enantiocomplementary protein AtDIR6, (−)-2 (78 % ee). Preferential formation (+)-2 observed in FiDIR1, whereas only racemic 2 absence proteins. The oxidative phenols marks a key step biosynthesis lignans, flavonolignans, and alkaloids plays central role plant secondary metabolism.1...
Prohormone processing by subtilases A flower that has gone to seed will drop its petals in a regulated process called abscission. Schardon et al. analyzed the production of peptide hormone regulates floral organ abscission model plant Arabidopsis thaliana. They used tissue-specific expression proteinase inhibitors identify subtilisin-like proteinases act as prohormone convertases required for plants. Science , this issue p. 1594
ABSTRACT In this study, we investigated the metabolism of ethylene glycol in Pseudomonas putida strains KT2440 and JM37 by employing growth bioconversion experiments, directed mutagenesis, proteome analysis. We found that strain grew rapidly with as a sole source carbon energy, while did not grow within 2 days incubation under same conditions. However, experiments revealed both strains, temporal accumulation glycolic acid glyoxylic for KT2440. This was further increased targeted mutagenesis....
Summary Peptide hormones are implicated in many important aspects of plant life and usually synthesized as precursor proteins. In contrast to animals, data for peptide hormone maturation scarce the specificity processing enzyme(s) is largely unknown. Here we tested a hypothesis that prosystemin, tomato ( Solanum lycopersicum ) wound systemin, performed by phytaspases, aspartate‐specific proteases subtilase family. Following purification phytaspase from leaves, two genes were identified,...
RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct kinases-hRio1, hRio2, and hRio3, which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 like are associated precursors 40S subunits human cells. Furthermore, demonstrate depletion by RNA interference affects the last step 18S rRNA maturation causes defects recycling several trans-acting factors (hEnp1, hLtv1, hDim2/PNO1, hNob1) from...
Abstract Background Plipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, application of this has so far been investigated in few cases, principally because yield low concentration and unknown regulation biosynthesis pathways. B. subtilis synthesizes plipastatin by non-ribosomal peptide synthetase encoded ppsABCDE operon. In study, 3NA (a non-sporulation strain) was engineered gain...
Wheat is of high importance for a healthy and sustainable diet the growing world population, partly due to its mineral content. However, several minerals are bound in phytate complex grain unavailable humans. We performed series trials compare contents phytic acid as well phytase activity varieties from alternative wheat species spelt, emmer einkorn with common wheat. Additionally, we investigated potential recent popular bread making recipes German bakeries reduce content, thus increase...
A transgenic plant cell suspension culture was established as a versatile and efficient expression system for the subtilase SlSBT3 from tomato. The recombinant protease purified to homogeneity supernatants by fractionated ammonium sulfate precipitation, batch adsorption cation exchange material, anion chromatography. Purified identified 79-kDa glycoprotein with both complex paucimannosidic type glycan chains at Asn(177), Asn(203), Asn(376), Asn(697), Asn(745). found be very stable enzyme,...
Abstract Gossypol is a defense compound in cotton plants for protection against pests and pathogens. biosynthesis involves the oxidative coupling of hemigossypol results two atropisomers owing to hindered rotation around central binaphthyl bond. (+)‐Gossypol predominates vivo, thus suggesting stereochemically controlled biosynthesis. The aim was identify factors mediating (+)‐gossypol formation investigate their potential asymmetric biaryl synthesis. A dirigent protein from Gossypium...
Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites applications in agriculture. Therefore, identification of productive wild-type is essential the development biopesticides. velezensis FZB42 a well-established strain biocontrol plant pathogens Here, we characterized an alternative strain, B. UTB96, that higher amounts all three major lipopeptide families, namely surfactin, fengycin, and iturin. UTB96 produces...
Surfactin is described as a powerful biosurfactant and natively produced by Bacillus subtilis in notable quantities. Among other industrially relevant characteristics, antimicrobial properties have been attributed to surfactin-producing isolates. To investigate this property, stress approaches were carried out with biotechnologically established strains of Corynebacterium glutamicum, subtilis, Escherichia coli Pseudomonas putida the highest possible amounts surfactin. Contrary popular...
Most peptide hormones and growth factors are matured from larger inactive precursor proteins by proteolytic processing further posttranslational modification. Whether or how modifications contribute to bioactivity is still largely unknown. We address this question here for TWS1 (Twisted Seed 1), a regulator of embryonic cuticle formation in Arabidopsis thaliana . Using synthetic peptides encompassing the N- C-terminal sites recombinant as substrates, we show that cleaved subtilase SBT1.8 at...
Bone mass is maintained by the balance between osteoclast-induced bone resorption and osteoblast-triggered formation. In inflammatory arthritis such as rheumatoid (RA), however, increased osteoclast differentiation activity skew this resulting in progressive loss. O-GlcNAcylation a posttranslational modification with attachment of single O-linked β-D-N-acetylglucosamine (O-GlcNAc) residue to serine or threonine residues target proteins. Although one most common protein modifications, its...
This study investigated the influence of bacterial cyclic lipopeptides (LP; surfactins, iturins, fengycins) on microbial interactions. The objective was to investigate whether presence bacteria inhibits fungal growth and this inhibition is due release metabolites, particularly LP. Selected endophytic strains with known plant-growth promoting potential were cultured in Fusarium oxysporum f.sp. strigae (Fos), which applied as model organism. extracellular metabolome tested bacteria, a focus...
This study investigates the potential of diatom Phaeodactylum tricornutum (PT) as a sustainable and nutritionally valuable food source, focusing on its ability to produce bioactive compounds such eicosapentaenoic acid, fucoxanthin, chrysolaminarin (CRY) proteins. PT was cultivated in flat-plate airlift photobioreactor (FPA-PBR) illuminated with LEDs from two sides. The aimed monitor minimize β-methylamino-L-alanine (BMAA) levels address safety concerns. data showed that selected FPA-PBR...
In a scenario of increasing alarm about food waste due to rapid urbanization, population growth and lifestyle changes, this study aims explore the valorization from retail sector as potential substrates for biotechnological production biosurfactants. With perspective increasingly contributing realization circular bioeconomy, vegetable juice, derived unsold fruits vegetables, carbon source was used produce lipopeptides such surfactin fengycin. The results shake flask cultivations revealed...
The Drosophila phototransduction cascade terminates in the opening of an ion channel, designated transient receptor potential (TRP). TRP has been shown to become phosphorylated vitro, suggesting regulation channel through posttranslational modification. However, except for one phosphorylation site, Ser982, which was analyzed by functional vivo studies (Popescu, D. C., Ham, A. J., and Shieh, B. H. (2006) J. Neurosci. 26, 8570–8577), nothing is known about role vivo. Here, we report...
Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol. By an unknown mechanism, they direct coupling radicals toward formation (+)- or (-)-pinoresinol. We show here that dirigent protein AtDIR6 Arabidopsis thaliana is a homodimeric all-beta in superfamily calycins. Based its homology with calycins, structure was modeled using allene oxide cyclase as template. The structural model...
Escherichia coli is a convenient host for the expression of proteins, but heterologous production large membrane protein complexes often hampered by lack specific accessory genes required insertion or cofactor assembly. In this study we introduce non-pathogenic and fast-growing Vibrio natriegens as suitable membrane-bound proteins from cholerae. We achieved primary Na+ pump, NADH:quinone oxidoreductase (NQR), V. cholerae in an active state, indicated increased overall oxidoreduction activity...
Tight regulation of inflammatory cytokine and interferon (IFN) production in innate immunity is pivotal for optimal control pathogens avoidance immunopathology. The human Nod-like receptor (NLR) NLRP11 has been shown to regulate type I IFN pro-inflammatory responses. Here, we identified the ATP-dependent RNA helicase DDX3X as a novel binding partner NLRP11, using co-immunoprecipitation LC-MS/MS. known enhance responses NLRP3 inflammasome activation. We demonstrate that can abolish...
In contrast to mammalian matrix metalloproteinases (MMPs) that play important roles in the remodeling of extracellular animals, proteases responsible for dynamic modifications plant cell wall are largely unknown. A possible involvement MMPs was addressed by cloning and functional characterization Sl2-MMP Sl3-MMP from tomato (Solanum lycopersicum). The two were found resemble homologs with respect gelatinolytic activity, substrate preference hydrophobic amino acids on both sides scissile...