A5075956136- Polysaccharides and Plant Cell Walls
- Neurogenesis and neuroplasticity mechanisms
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Carbohydrate Chemistry and Synthesis
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Neuroinflammation and Neurodegeneration Mechanisms
- Glycosylation and Glycoproteins Research
- Fermentation and Sensory Analysis
- Enzyme Catalysis and Immobilization
- Analytical Chemistry and Chromatography
- Cytomegalovirus and herpesvirus research
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Neuroscience and Neuropharmacology Research
- Amino Acid Enzymes and Metabolism
- Regulation of Appetite and Obesity
- Phytochemistry and Bioactive Compounds
- Olfactory and Sensory Function Studies
- Sleep and Wakefulness Research
- Genetics and Neurodevelopmental Disorders
- Biochemical and Molecular Research
- Neuroendocrine regulation and behavior
- Biopolymer Synthesis and Applications
Institut de Neurobiologie de la Méditerranée
2011-2024
Inserm
2012-2024
Aix-Marseille Université
2012-2024
Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille
2012-2019
Centre National de la Recherche Scientifique
2000-2014
Centre de Gestion Scientifique
2009
California Institute of Technology
2000-2006
Université Claude Bernard Lyon 1
2000-2003
École Normale Supérieure de Lyon
2000
Slovak Academy of Sciences
1971-1986
Although neural stem cells (NSCs) persist in various areas of the adult brain, their contribution to brain repair after injury is very limited. Treatment with exogenous growth factors can mitigate this limitation, suggesting that environment normally deficient permissive cues and it may be possible stimulate latent regenerative potential endogenous progenitors appropriate signals. We analyzed effects overexpressing cytokine leukemia inhibitory factor (LIF) on neurogenesis normal brain. found...
The mammalian olfactory epithelium (OE) is composed of primary sensory neurons (OSNs) that are renewed throughout adulthood by local, restricted neuronal progenitor cells. molecular signals control this neurogenesis in vivo unknown. Using bulb ablation (OBX) adult mice to trigger synchronous mitotic stimulation progenitors the OE, we show involvement a cytokine cellular events leading regeneration OE. We find that, many potential mitogenic signals, only leukemia inhibitory factor (LIF)...
The effect of 2-deoxy-d-glucose and cycloheximide on the synthesis secretion cell-wall constituents protein mannan in yeast protoplasts was examined detail. Although hardly influenced synthesis, a significant parallel inhibition carbohydrate into medium observed. mechanism this is considered as an interference metabolites with mannan. Cycloheximide, which effective inhibitor (Kerridge, 1958), inhibited non-diffusible protoplasts, but other hand had no activity particulate synthetase. Our...
Adult neurogenesis is studied in vivo using thymidine analogues such as bromodeoxyuridine (BrdU) to label DNA synthesis during the S phase of cell cycle. However, BrdU may also events not directly related proliferation, repair and/or abortive reentry into cycle, which can occur part an apoptotic process postmitotic neurons. In this study, we used three well-characterized models injury-induced neuronal apoptosis and combined visualization birth (BrdU labeling) death (Tdt-mediated dUTP-biotin...
The incorporation of mannan labeled by d -[1- 3 H]mannose into the growing cell walls Saccharomyces cerevisiae has been followed means high-resolution autoradiography. results show that, in very first stage budding, when shape bud is nearly spherical, new incorporated uniformly over whole surface emerging bud. Later on, as growth proceeds, polarized tip predominates. In maturation phase growth, size about two-thirds that mother cell, again a considerable substrate was observed. wall remained...
The growth inhibition and the lysis of Saccharomyces cerevisiae caused by 2-deoxy- d -glucose (2-DG) were shown to be a consequence unbalanced cellular division. lysis, but not repression osmotic fragility cells, could suppressed addition mannitol as an stabilizer. This result, well morphological changes observed in cells chemical composition cell walls, showed that S. grown presence 2-DG formed weakened walls responsible for fragility. Evidence is presented first time demonstrating...
Spinal cord injury (SCI) triggers a complex cellular response at the site, leading to formation of dense scar tissue. Despite this local tissue remodeling, consequences SCI level in distant rostral sites (i.e., brain), remain unknown. In study, we asked whether cervical could alter cell dynamics neurogenic areas adult rat forebrain. To aim, quantified BrdU incorporation and determined phenotypes newly generated cells (neurons, astrocytes, or microglia) during subchronic chronic phases...
The hypothalamus plays a crucial role in the control of energy balance and also retains neurogenic potential into adulthood. Recent studies have reported severe alteration cell turn-over obese animals it has been proposed that deficiency could be involved development obesity. To explore this possibility, we examined hypothalamic renewal during homeostatic response to dietary fat mice, i.e., at onset diet-induced We found switching high-fat diet (HFD) accelerated through local, rapid...
Background: Congenital cytomegalovirus (CMV) infections are one leading cause of human neurodevelopmental disorders. Increasing evidence for the pathogenic involvement brain immune alterations was obtained in recent years. Host and virus-encoded chemokines might play important roles CMV-related neuropathogenesis by regulating leukocyte trafficking microglia recruitment CMV-infected brains, interfering with key steps. In a rat model CMV infection fetal utero that leads to detrimental...
Leptin is an adipocyte-derived hormone that controls energy balance by acting primarily in the CNS, but its action lost common forms of obesity due to central leptin resistance. One potential mechanism for such resistance increased hypothalamic expression Suppressor cytokine signaling 3 (Socs3), a feedback inhibitor Jak-Stat pathway prevents Stat3 activation. Ample studies have confirmed important role Socs3 and obesity. However, degree which participates regulation homeostasis nonobese...
2-Deoxy- d -glucose (2DG) acted as a competitive inhibitor of the synthesis cell wall components in Saccharomyces cerevisiae protoplasts. The fibrillar glucan component was inhibited at glucose to 2DG ratio 4:1 cultivating medium. completion formation by amorphous mannan-protein about 20:1. inhibition could be reversed increasing nutrient No incorporation into observed.
The epilepsy-aphasia spectrum (EAS) is a heterogeneous group of age-dependent childhood disorders characterized by sleep-activated discharges associated with infrequent seizures and language, cognitive, behavioral deficits. Defects in the GRIN2A gene, encoding subunit glutamate-gated N-methyl-d-aspartate (NMDA) receptors, represent most important cause EAS identified so far. Neocortical or thalamic lesions were detected subset severe disorders, more subtle anomalies reported patients...
The effect of a synthetic glucose analogue, 2-deoxy-2-fluoro- d -glucose (FG) on growth and metabolism Saccharomyces cerevisiae was studied. addition FG (0.005-0.05%) to 2% medium resulted in reduction the initial rate and, after several hours, complete cessation culture growth. These two events were due extensive lysis population which continued long period when no more recorded. Electron microscope examination lysed cells showed that consequence dissolution cell walls. inhibited similar...