Surfactant protein A (SP-A) enhances phagocytosis of Pseudomonas aeruginosa. SP-A1 and SP-A2 encode human (h) SP-A; products enhance more than SP-A1. Oxidation can affect SP-A function. We hypothesized that in vivo vitro ozone-induced oxidation (as assessed by its carbonylation level) negatively affects function bacteria cell association). To test this, we used P. aeruginosa, rat alveolar macrophages (AMs), hSP-As with varying levels (natural) oxidation, ozone-exposed (1A, 1A0) (6A2, 6A4)...

Chronic airway inflammation caused by Pseudomonas aeruginosa is an important feature of cystic fibrosis (CF). Surfactant protein A (SP-A) enhances phagocytosis P. aeruginosa. Two genes, SP-A1 and SP-A2, encode human SP-A. We hypothesized that genetically determined differences in the activity SP-A2 gene products exist. To test this, we studied association a nonmucoid strain (ATCC 39018) with rat alveolar macrophages presence or absence insect cell-expressed SP-A variants. used two trios,...

Sex differences have been described in a number of pulmonary diseases. However, the impact ozone exposure followed by pneumonia infection on sex-related survival and macrophage function not reported. The purpose this study was to determine whether differentially affects: 1) male female mice infected with Klebsiella pneumoniae, 2) phagocytic ability macrophages from these mice.Male C57BL/6 were exposed O3 or filtered air (FA) (control) then intratracheally K. pneumoniae bacteria. Survival...

Surfactant protein A (SP-A) enhances phagocytosis of Pseudomonas aeruginosa. Two functional genes, SP-A1 and SP-A2, encode human SP-A. As we showed before, baculovirus-mediated insect cell-expressed SP-A2 the association P. aeruginosa with rat alveolar macrophages (rAMs) more than does SP-A1. However, true (internalization) was not shown, cell derived proteins lack or are defective in certain mammalian posttranslational modifications that may be important for activity specificity. Here used...

Abstract Background Surfactant protein A (SP-A) enhances phagocytosis of bacteria, including Klebsiella pneumoniae , by alveolar macrophages. Ozone, a major air pollutant, can cause oxidation surfactant and may influence lung immune function. Immune function also be affected sex-specific mechanisms. We hypothesized that ablation SP-A has negative impact on the susceptibility mice to infection after ozone exposure, sex differences in effect do exist. Methods Male female (-/-) C57BL/6J...

Introduction. Currently, a promising direction in water sample toxicity research involves conducting screening analysis on human cell lines. The purpose of this study is to assess the impact model drinking pollutant metabolic activity duodenal adenocarcinoma line HuTu-80 justify its further use as test object for evaluation samples. Materials and methods. standard culture HuTu-80. To toxic effect chloride, sulfate, nitrate ions culture, their sodium salts were used: NaCl concentrations...

The human surfactant protein A (SP-A) locus consists of two functional genes (SP-A1, SP-A2) with gene-specific products exhibiting qualitative and quantitative differences. aim here was twofold: 1) generate SP-A1 antibody, 2) use this to assess SP-A content in the bronchoalveolar lavage fluid (BALF). An SP-A1-specific polyclonal antibody (hSP-A1_Ab(68-88)_Col) raised chicken, its specificity determined by immunoblot ELISA using mammalian Chinese hamster ovary (CHO) cell-expressed SP-A2...

Survival of mice after Klebsiella pneumoniae infection and phagocytosis by alveolar macrophages (AMs), in the presence or absence ozone (O3) exposure prior to infection, is sex dependent. The objective this work was study role gonadal hormones, 5α-dihydrotestosterone (DHT) 17β-estradiol (E2), on mouse survival filtered air (FA) O3 exposure. Gonadectomized female (G×F) male (G×M) implanted with control hormone pellets (DHT G×F, E2 G×M), exposed (2 ppm, 3h) FA, infected K. were monitored for...

Four "core" amino acid differences within the collagen-like domain distinguish human surfactant protein A1 (SP-A1) variants from SP-A2 variants. One of these, cysteine 85 that could form intermolecular disulfide bonds, is present in SP-A1 (Cys85) and absent (Arg85). We hypothesized residue affects both structure function To test this, wild-type (WT) variants, 6A2 1A0 SP-A2, their mutants (6A2(C85R) 1A0(R85C)) were generated studied. found following: (1) Residue affected binding ability to...

Introduction. Harmful inhalation effects in the workplace, most of which are preventable, determine more than 10% chronic non-infectious bronchopulmonary pathology over world at population level. In this regard, updated information on risk factors various sectors economy and prevalence diseases associated with them is important for scientific justification targeted programs prevention occupational respiratory diseases. Materials methods. On basis competent statistical materials...

Surfactant protein A (SP-A) plays critical roles in host defense, regulation of inflammation and surfactant metabolism the lung. The human SP-A locus consists two functional genes, SFTPA1 SFTPA2 encoding proteins SP-A1 SP-A2, respectively. Structural differences exist between SP-A2 vitro vivo. Ozone is a major air pollutant with negative impact on many biological processes. In this study we used humanized transgenic (hTG) mice, KO mice to vivo effects bronchoalveolar lavage (BAL) proteomic...

Introduction. Along with physical, chemical, ergonomic, and biological factors, working psychosocial stress is one of the main risks for development metabolic syndrome (MS), system effects which can cause severe lesions cardiovascular, endocrine, hepatobiliary systems. The purpose work was to summarize analyze modern domestic foreign experience studying relationship between categories factors environment MS its components in different occupational cohorts. publications were searched through...

Surfactant protein A (SP-A) plays an important role in innate immunity. The sex-dependent survival of infected SP-A knockout (KO) mice has been observed. Our goal was to study the impact ozone (O3) and sex, as well gonadal hormones, on bronchoalveolar lavage (BAL) readouts survival, respectively, Klebsiella pneumoniae-infected KO mice. Male female were exposed O3 or filtered air with K. pneumoniae. We studied markers inflammation tissue damage at 4, 24, 48 h, over 14 days, gonadectomized...

Objective of this study was the molecular genetic characteristics uropathogenic strains Escherichia coli , isolated from urine patients with urinary tract infections in Saratov City, order to determine phylogenetic groups and subgroups which belong, identify markers associated virulence. Materials methods . Molecular 102 E. were infections, performed. PCR used frequency oc-currence genes ( fimH, pap, sfa, afa, iha, irp2, iuc, iroN, hlyA, vat, usp, set-1, kpsMT, iss, cva, ompT ) factors...

The review concentrates on generalizing results produced by contemporary studies relations between chronic occupational stress and a wide range of biomarkers homeostasis functional state the body. Bearing in mind diagnostic predictive significance neuroendocrine, neurophysiologic, immune metabolic stress, we analyzed sixty two published papers that were best suitable for our goals. These works selected from one hundred eighty seven information sources available CyberLeninka, PubMed Google...

This paper analyzes the parasitological state of natural water bodies — rivers, lakes, springs, wells, as sources drinking supply, according to content round and flat helminthes. It is shown that climate aridization, which has been taking place in recent decades, causes, first all, a decrease hygienic safety surface an increase number some helminth eggs them, particular, cestodes, associated with average annual temperature under conditions aridization. The unfavorable composition presence...

Introduction: Statistics show that over 11 million Russians use untreated tap water noncompliant with safety standards. Consumption of such may have adverse human health effects and pose risks various diseases. The objective this study was to investigate the relationship between consumption artesian sourced from centrally operated supply systems in arid areas Saratov Region prevalence cardiovascular, urogenital, digestive diseases local population. Materials methods: We examined 127 samples...