A5076128010- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Mycorrhizal Fungi and Plant Interactions
- Fungal and yeast genetics research
- Entomopathogenic Microorganisms in Pest Control
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Plant Pathogens and Resistance
- Mycotoxins in Agriculture and Food
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Essential Oils and Antimicrobial Activity
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant and animal studies
- Plant tissue culture and regeneration
- Insect Pest Control Strategies
- Antifungal resistance and susceptibility
- Plant responses to elevated CO2
- Insect symbiosis and bacterial influences
- Plant Reproductive Biology
- Biochemical and Structural Characterization
- Polysaccharides and Plant Cell Walls
University of Naples Federico II
2006-2024
Federico II University Hospital
2021-2024
University of Córdoba
2012-2023
As the use of RNA-seq has popularized, there is an increasing consciousness importance experimental design, bias removal, accurate quantification and control false positives for proper data analysis. We introduce NOISeq R-package quality analysis count data. show how available diagnostic tools can be used to monitor issues, make pre-processing decisions improve demonstrate that non-parametric NOISeqBIO efficiently controls discoveries in experiments with biological replication outperforms...
In plants, innate immune responses are initiated by plasma membrane-located pattern recognition receptors (PRRs) upon of elicitors, including exogenous pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated (DAMPs). Arabidopsis thaliana produces more than 1000 secreted peptide candidates, but it has yet to be established whether any these act as elicitors. Here we identified an A. gene family encoding precursors PAMP-induced peptides (prePIPs) through in-silico...
Plant roots release complex mixtures of bioactive molecules, including compounds that affect the activity and modify composition rhizosphere microbiome. In this work, we investigated initial phase interaction between tomato an effective biocontrol strain Trichoderma harzianum (T22). We found root exudates (RE), obtained from plants grown in a split-root system exposed to various biotic abiotic stress factors (wounding, salt, pathogen attack), were able stimulate growth act as...
Successful biocontrol interactions often require that the beneficial microbes involved are resistant or tolerant to a variety of toxicants, including antibiotics produced by themselves phytopathogens, plant antimicrobial compounds, and synthetic chemicals contaminants. The ability Trichoderma spp., most widely applied fungi, withstand different chemical stresses, those associated with mycoparasitism, is well known. In this work, we identified an ATP-binding cassette transporter cell membrane...
Abstract Soilborne fungal pathogens cause devastating yield losses and are highly persistent difficult to control. During the infection process, these organisms must cope with limited availability of iron. Here we show that bZIP protein HapX functions as a key regulator iron homeostasis virulence in vascular wilt fungus Fusarium oxysporum. Deletion hapX does not affect uptake but causes derepression genes involved iron-consuming pathways, leading impaired growth under iron-depleted...
Fungi belonging to the genus Trichoderma are among most active and ecologically successful microbes found in natural environments, because they able use a variety of substrates affect growth other virtually any plant species. We isolated characterized novel type II hydrophobin secreted by biocontrol strain MK1 longibrachiatum. The corresponding gene (Hytlo1) has multiple role Trichoderma–plant–pathogen three-way interaction, while purified protein displayed direct antifungal as well...
Mitogen-activated protein kinase (MAPK) cascades mediate cellular responses to environmental signals. Previous studies in the fungal pathogen Fusarium oxysporum have revealed a crucial role of Fmk1, MAPK orthologous Saccharomyces cerevisiae Fus3/Kss1, vegetative hyphal fusion and plant infection. Here, we genetically dissected individual combined contributions three MAPKs Mpk1 Hog1 regulation development, stress response virulence F. on animal hosts. Mutants lacking Fmk1 or were affected...
Abstract Fusarium oxysporum is a cross-kingdom fungal pathogen that infects plants and humans. Horizontally transferred lineage-specific (LS) chromosomes were reported to determine host-specific pathogenicity among phytopathogenic F. . However, the existence functional importance of LS human pathogenic isolates are unknown. Here we report four unique in strain NRRL 32931, isolated from leukemia patient. These devoid housekeeping genes, but significantly enriched genes encoding metal ion...
Summary Soil‐inhabiting fungal pathogens use chemical signals to locate and colonise the host plant. In vascular wilt fungus Fusarium oxysporum , hyphal chemotropism towards tomato roots is triggered by secreted plant peroxidases (Prx), which catalyse reductive cleavage of reactive oxygen species (ROS). Here we show that this chemotropic response requires regulated synthesis ROS conserved NADPH oxidase B (NoxB) complex, their transformation into hydrogen peroxide (H 2 O ) superoxide...
Soil-inhabiting fungal pathogens use chemical signals released by roots to direct hyphal growth towards the host plant. Whether other soil microorganisms exploit this capacity for their own benefit is currently unknown. Here we show that endophytic rhizobacterium Rahnella aquatilis locates hyphae of root-infecting pathogen Fusarium oxysporum through pH-mediated chemotaxis and uses them as highways efficiently access colonize plant roots. Secretion gluconic acid (GlcA) R. in rhizosphere leads...
Trichoderma atroviride is a mycoparasitic fungus used as biological control agent to protect plants against fungal pathogens. Successful biocontrol based on the perception of signals derived from both plant symbiont and prey. Here, we applied three different chemotropic assays study chemosensing capacity T. toward compounds known or suspected play role in mycoparasite/plant host/prey interactions cover complete spectrum developmental stages. Purified compounds, including nutrients, secondary...
Endophytic fungi (EF) can enhance both plant growth and defense barriers against pests pathogens, contributing to the reduction of chemical pesticides fertilizers use in agriculture. Beauveria bassiana is an entomopathogenic fungus showing endophytism several crops, often associated with a good capacity limit development disease agents. However, diversity protective efficacy response different strains be remarkable needs carefully assessed for successful predictable these beneficial...
Abstract Fungal interactions with plant roots, either beneficial or detrimental, have a crucial impact on agriculture and ecosystems. The cosmopolitan pathogen Fusarium oxysporum (Fo) provokes vascular wilts in more than hundred different crops. Isolates of this fungus exhibit host-specific pathogenicity, which is conferred by lineage-specific Secreted In Xylem (SIX) effectors encoded accessory genomic regions. However, such isolates also can colonize the roots other plants asymptomatically...
Super-resolution microscopy has evolved as a powerful method for subdiffraction-resolution fluorescence imaging of cells and cellular organelles, but requires sophisticated expensive installations. Expansion (ExM), which is based on the physical expansion structure interest, provides cheap alternative to bypass diffraction limit enable super-resolution conventional microscope. While ExM shown impressive results magnified visualization proteins RNAs in tissues, it not yet been applied fungi,...
During an infection, a single or multispecies biofilm can develop. Infections caused by non-dermatophyte molds, such as Fusarium spp. and yeasts, Candida spp., are particularly difficult to treat due the formation of mixed two species. oxysporum is responsible for approximately 20% human fusariosis, while albicans superficial mucosal dermal infections disseminated bloodstream with mortality rate above 40%. This study aims investigate interactions between C. F. dual-species biofilm,...
Fungal phytopathogens cause devastating losses in global agriculture. All plant-infecting fungi use conserved MAPK signaling pathways to successfully locate, enter, and colonize their hosts.
Verticillium wilts caused by spp. are among the most challenging plant diseases to control and affect numerous hosts worldwide. Due lack of effective, conventional methods, integrated strategies provide a promising approach manage these diseases. The non-pathogenic Fusarium oxysporum strain FO12 was reported in previous studies be an effective biocontrol agent against dahliae, however its mode action remains elucidated. In this study, complementary vitro vivo experiments were conducted order...
Fusaric acid (FA) is one of the first secondary metabolites isolated from phytopathogenic fungi belonging to genus Fusarium. This molecule exerts a toxic effect on plants, rhizobacteria, and animals, it plays crucial role in both plant animal pathogenesis. In metal chelation by FA considered possible mechanisms action. Here, we evaluated different nitrogen sources, iron content, extracellular pH cellular signalling pathways production siderophores pathogen Fusarium oxysporum (Fol). Our...