A5007150931- Plant Virus Research Studies
- Cassava research and cyanide
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Plant Micronutrient Interactions and Effects
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Potato Plant Research
- Plant responses to water stress
- Soybean genetics and cultivation
- Seed Germination and Physiology
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Transgenic Plants and Applications
- Genetic and Environmental Crop Studies
- Banana Cultivation and Research
- Phytase and its Applications
- Phytoplasmas and Hemiptera pathogens
- Bacteriophages and microbial interactions
- Cellular transport and secretion
Institute for Sustainable Plant Protection
2014-2024
National Research Council
2019-2023
International Institute of Tropical Agriculture
2017-2023
International Institute of Tropical Agriculture
2016-2023
Centro di Ricerca per la Patologia Vegetale
2010-2014
Friedrich Miescher Institute
2001-2005
Centre hospitalier universitaire de Québec
2001
RNA silencing plays a critical role in plant resistance against viruses. To counteract host defense, viruses encode viral suppressors of (VSRs) that interfere with the cellular machinery through various mechanisms not always well understood. We examined Mungbean yellow mosaic virus (MYMV) AC4 and showed it is essential for infectivity but replication. It acts as determinant pathogenicity counteracts induced gene by strongly suppressing systemic phase whereas does local production siRNA....
Cassava storage roots are among the most important root crops worldwide, and represent one of consumed staple foods in sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous from its stem. These formed through activation secondary growth processes. However, underlying genetic regulation development is largely unknown. Here we report distinct structural transcriptional changes occurring during early phases development. A pronounced increase auxin-related...
The transport of a viral genome from cell to is enabled by movement proteins (MPs) targeting the periphery mediate gating plasmodesmata. Given their essential role in development infection, understanding regulation MPs great importance. Here, we show that cauliflower mosaic virus (CaMV) MP contains three tyrosine-based sorting signals interact with an Arabidopsis (Arabidopsis thaliana) μA-adaptin subunit. Fluorophore-tagged incorporated into vesicles labeled endocytic tracer...
Cassava is an important staple crop in sub-Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this are poorly understood including the mode of photosynthesis, reasons for rate extent source/sink limitation, impact environment, and variation between cultivars. Six commercial African cassava cultivars were grown a greenhouse Erlangen, Germany, field Ibadan, Nigeria. Source leaves, sink stems storage roots harvested during root...
The function of the virion-associated protein (VAP) cauliflower mosaic virus (CaMV) has long been only poorly understood. VAP is associated with virion but dispensable for morphogenesis and replication. It mediates transmission by aphids through simultaneous interaction both aphid factor virion. However, although insect not fundamental to CaMV survival, indispensable spreading infection within host plant. We used a GST pull-down technique demonstrate that interacts viral movement coiled-coil...
Despite the importance of storage root (SR) organs for cassava and other crops yield, their developmental origin is poorly understood. Here we use multiple approaches to shed light on initial stages development demonstrating that SR fibrous roots (FR) follow different rhizogenic processes. Transcriptome analysis carried out collected before, during after bulking highlighted early specific activation a number functions essential swelling identified root-specific genes able effectively...
Cassava (Manihot esculenta Crantz) is a vital tropical root crop providing essential dietary energy to over 800 million people in and subtropical regions. As climate-resilient crop, its significance grows as the human population expands. However, yield improvement faces challenges from biotic abiotic stress limited breeding. Advanced sequencing assembly techniques enabled generation of highly accurate, nearly complete, haplotype-resolved genome African cassava cultivar TMEB117. It most...
Dioecy (distinct male and female individuals) scarce to non-flowering are common features of cultivated yam (Dioscorea spp.). However, the molecular mechanisms underlying flowering sex determination in Dioscorea largely unknown. We conducted SuperSAGE transcriptome profiling male, monoecious individuals identify sex-related genes white Guinea (D. rotundata), generating 20,236 unique tags. Of these, 13,901 were represented by a minimum 10 A total 88 tags significantly differentially expressed...
ABSTRACT All plant pararetroviruses belong to the Caulimoviridae family. This family contains six genera of viruses with different biological, serological, and molecular characteristics. Although some important mechanisms viral replication host infection are understood, much remains be discovered about many functions proteins. The focus this study, virion-associated protein (VAP), is conserved among all members group a coiled-coil structure that has been shown assemble as tetramer in case...
Plant viruses move through plasmodesmata (PD) either as nucleoprotein complexes (NPCs) or tubule-guided encapsidated particles with the help of movement proteins (MPs). To explore how and why MPs specialize in one mechanism other, we tested exchangeability encoded by DNA RNA virus genomes means an engineered alfalfa mosaic (AMV) system. We show that Caulimoviridae (DNA genome virus) are competent for particle transport but unable to mediate NPC movement, discuss this restriction terms...
Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of parqui mosaic disease. The genome was cloned and clone proven to be infectious C. parqui. presence typical viroplasms in virus-infected plant tissue information obtained from complete genomic sequence confirmed CmYLCV a member Caulimoviridae family. All characteristic domains conserved pararetroviruses were found CmYLCV. Its is 8253 bp long contains seven open reading frames (ORFs). Phylogenetic...
ABSTRACT Cauliflower mosaic virus (CaMV) open reading frame III (ORF III) codes for a virion-associated protein (Vap), which is one of two viral proteins essential aphid transmission. However, unlike the transmission factor encoded by CaMV ORF II, Vap also systemic infection, suggesting that it multifunctional protein. To elucidate additional function or functions Vap, we tested replication noninfectious III-defective mutants in transfected turnip protoplasts. PCR and Western blot analyses...
Mulberry badnavirus 1 (MBV1) has been characterized as the aetiological agent of a disease observed on mulberry tree in Lebanon (accession L34). A small RNA next-generation sequencing library was prepared and analysed from L34 extract, these data together with genome walking experiments have used to obtain full-length virus sequence. Uniquely among badnaviruses, MBV1 sequence encodes single ORF containing all conserved pararetrovirus motifs. Two sizes (6 kb 7 kb) were found be encapsidated...
Systemin is a plant signal peptide hormone involved in the responses to wounding and insect damage Solanaceae family. It works same signaling pathway of jasmonic acid (JA) enhances expression proteinase inhibitors. With aim studying role for systemin antiviral responses, tomato (Solanum lycopersicum) transgenic line overexpressing prosystemin cDNA, i.e. precursor, was inoculated with Cucumber mosaic virus (CMV) strain Fny supporting either necrogenic or non-necrogenic satellite RNA (satRNA)...
Cassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich carbohydrate, are deficient essential micronutrients, including provitamin A carotenoids. Increasing carotenoid content cassava roots important to reduce the incidence of vitamin deficiency, public health problem sub-Saharan Africa. However, improvement advances slowly, mainly due limited information on molecular factors influencing β-carotene accumulation cassava....
Abstract Dioecy (distinct male and female individuals) combined with scarce to non-flowering are common features of cultivated yam ( Dioscorea spp.). However, the molecular mechanisms underlying flowering sex determination in unknown. We conducted SuperSAGE transcriptome profiling male, monoecious individuals identify sex-related genes white Guinea D. rotundata ). analysis generated a total 20,236 unique tags, which 13,901 were represented by minimum 10 tags. Of these, 88 tags significantly...
The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin content major staple crops. Cassava holds much promise for food security Africa, but a negative correlation between β-carotene, carotenoid, and dry matter has been reported, which poses challenge cassava biofortification by conventional breeding. To identify suitable material genetic transformation tissue culture with the overall aim increase β-carotene maintain starch as well...