Plant salt tolerance can be improved by grafting onto salt-tolerant rootstocks. However, the underlying signaling mechanisms behind this phenomenon remain largely unknown. To address issue, we used a range of physiological and molecular techniques to study responses self-grafted pumpkin-grafted cucumber plants exposed 75 mM NaCl stress. Pumpkin significantly increased plants, as revealed higher plant dry weight, chlorophyll content photochemical efficiency (Fv/Fm), lower leaf Na+ content....

Potassium (K+) is a critical determinant of salinity tolerance, and H2O2 has been recognized as an important signaling molecule that mediates many physiological responses. However, the details how regulates K+ uptake in root under salt stress remain elusive. In this study, salt-sensitive cucumber salt-tolerant pumpkin which belong to same family, Cucurbitaceae, were used answer above question. We show higher tolerance was related its superior ability for accumulation apex. Transcriptome...

Tissue tolerance to salinity stress is a complex physiological trait composed of multiple 'sub-traits' such as Na+ compartmentalization, K+ retention, and osmotic tolerance. Previous studies have shown that some Cucurbita species employ tissue combat we aimed identify the molecular mechanisms involved. Five C. maxima (salt-tolerant) five moschata (salt-sensitive) genotypes were comprehensively assessed for their salt results showed tissue-specific transport characteristics enabled more...

A fungus Fusarium oxysporum F. sp. niveum (FON) is the causal organism of wilt in watermelon. In this study, we evaluated effect wheat intercropping on Our results showed that decreases incidence watermelon, likely due to secretion coumaric acid from roots dramatically inhibits FON spore germination, sporulation, and growth. The p-hydroxybenzoic acid, ferulic cinnamic watermelon stimulates phenolic acids organic also promoted by infection. However, substantially reduced under systems....

Abstract Cucurbit crops are suitable models for studying long-distance signaling in horticultural plants. Although thousands of substances graft transmissible cucurbits, functional studies have been hampered by the lack efficient genetic transformation systems. Here, we report a convenient and root method several cucurbit that will facilitate genes shoot–root crosstalk. We obtained healthy plants with completely transformed roots non-transgenic shoots within 6 weeks. Furthermore, combined...

Drought, cold and salinity are the major environmental stresses that limit agricultural productivity. NAC transcription factors regulate stress response in plants. Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop it has strong resistance to abiotic stress; however, biological functions of stress-related genes this largely unknown. This study reports function CmNAC1, a stress-responsive pumpkin domain protein. The CmNAC1-GFP fusion protein was transiently expressed tobacco...

Pumpkin is of great economic importance not only as food resources but also the most widely used rootstock to graft cucurbit crops. improves salt tolerance cucumber scion through respiratory burst oxidase homolog protein D (CmRbohD)-mediated reactive oxygen species (ROS) burst, which further enhances Na+ export and K+ uptake. RbohD activation requires calcium signaling. However, underlying mechanism remains largely unknown. In this study, we discovered two members from pumpkin involved in...

Soil salinity adversely affects the growth and yield of crops, including cucumber, one most important vegetables in world. Grafting with salt-tolerant pumpkin as rootstock effectively improves cucumber under different salt conditions by limiting Na⁺ transport from to scion. High-affinity potassium transporters (HKTs) are crucial for long distance plants, but function HKTs this process grafted plants remains unclear. In work, we have characterized CmHKT1;1 a member HKT gene family Cucurbita...

Abstract The NAC transcription factor is a type of plant-specific that can regulate plant salt tolerance, but the underlying mechanism unclear in grafted vegetables. H2O2 and ABA pumpkin rootstocks be transported to cucumber scion leaves, promoting stomatal closure improve tolerance cucumbers. Despite these observations, regulatory unknown. Here, our research revealed CmoNAC1 key regulates signaling roots under stress. function was analyzed using root transformation RNA-seq, we found...

In the early period under salt stress conditions, reduced stomatal opening could prevent water loss and avoid wilting. ABA signal plays an important role during this process. Here, we show that cucumber grafted onto pumpkin have a more rapid closure helps plant adapt to osmotic caused by salinity. Accelerated abscisic acid (ABA) contents in root, xylem sap leaf were evaluated for two grafting combinations (self-grafted rootstock), four transcript of signaling genes (NCED2, ABCG22, PP2C...

Vegetable crop quality and productivity are impaired by adverse light temperature stresses, such as low light, cold, heat stress. Alternative splicing is a powerful strategy for coping with these abiotic stresses producing multiple mRNA variants different subcellular locations, translation efficiency, coding sequences. Pre-mRNA alternatively spliced the spliceosome consisting of splicing-related proteins termed factors. Recent studies have shown that factors diverse effects on development,...

Low-light-stress is a common meteorological disaster that can result in slender seedlings. The photoreceptors play crucial role perceiving and regulating plants' tolerance to low-light-stress. However, the low-light-stress of cucumber has not been effectively evaluated, functions these photoreceptor genes cucumber, particularly under conditions, are clear.

ABSTRACT Cucumber blight is a destructive disease. The best way to control this disease resistance breeding. This study focuses on gene mapping and molecular marker development. We used the resistant cucumber, JSH, susceptible B80, as parents construct F2 populations. Bulked segregant analysis (BSA) combined with specific length amplified fragment sequencing (SLAF‐seq) were used, from which we developed cleaved polymorphic sequence (CAPs) markers map gene. Resistance in individuals showed...

Source-sink relationship, which was influenced by both genotype and environmental factors, contributed to the variation in photosynthesis photosynthate partitioning of wheat. Source reduction partial defoliation increased leaf net photosynthetic rate (PN), sink decreased PN irrigated However, change varied among genotypes. enhanced translocation into grain enhancement more evident cv. Lumai 215953 than incv. 15. Sink had little effect on 15, but it stem 215953. In rainfed, non-irrigated...

Sugar allocation is based on the source-to-sink and intracellular transport between different organelles, sugar transporters are usually involved in these processes. Tonoplast (TST) responsible for transporting into vacuoles; however, role of TSTs root growth response to abiotic stress poorly studied. Here, RNA analysis promoter-β-glucuronidase staining revealed that a melon TST1 gene (CmTST1) highly expressed roots. The feeding experiment results showed expression CmTST1 roots was induced...

The melon (Cucumis melo L.) is an economically important fruit crop around the world. Its colorful and diverse peels also possess remarkable aesthetic value. G2-like transcription factors (GLKs) play a pivotal role in regulating peel color. However, melon's GLKs have not been comprehensively analyzed. In this study, total of 37 CmGLK genes were identified further categorized into four distinct subfamilies: I, II, III, IV. organ-specific gene expression revealed that CmAPRR2;1 specifically...

Grafting can improve the salt tolerance of many crops. However, whereas critical genes in scions responsive to rootstock under stress remain a mystery. We found that pumpkin decreased content Na+ by 70.24%, increased K+ 25.9%, and K+/Na+ ratio 366.0% cucumber scion leaves. Furthermore, RNA-seq analysis showed ion transport-related were key responding grafted cucumbers. The identification expression channel proteins reveals 6 5 HAK5 members pumpkin, respectively. CsHAK5;3 was elevated...