A5019180595- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Ion channel regulation and function
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal and fetal brain pathology
- Polyamine Metabolism and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Vestibular and auditory disorders
- Endoplasmic Reticulum Stress and Disease
- Parkinson's Disease Mechanisms and Treatments
- Genetic Neurodegenerative Diseases
- Erythrocyte Function and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Hearing, Cochlea, Tinnitus, Genetics
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Mitochondrial Function and Pathology
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
Sage Therapeutics (United States)
2024
Harvard University
2018-2023
Massachusetts General Hospital
2018-2023
Erasmus MC
2016-2018
American Academy of Arts and Sciences
2014
Erasmus University Rotterdam
2011-2013
The rodent whisker system is widely used as a model for investigating sensorimotor integration, neural mechanisms of complex cognitive tasks, development, and robotics. pathways to the barrel cortex have received considerable attention. However, many subcortical structures are paramount system. They contribute important processes, like filtering out salient features, integration with other senses, adaptation general behavioral state animal. We present here an overview brain regions their...
Abstract Chloride homeostasis determines the impact of inhibitory synaptic transmission and thereby mediates excitability neurons. Even though cerebellar Purkinje cells (PCs) receive a pronounced GABAergic input from stellate basket cells, role chloride in these neurons is largely unknown. Here we studied at both cellular systems physiological level function recently discovered channel, SLC26A11 or kidney brain anion transporter (KBAT), which prominently expressed PCs. Using perforated patch...
Whisker-based object localization requires activation and plasticity of somatosensory motor cortex. These parts the cerebral cortex receive strong projections from cerebellum via thalamus, but it is unclear whether to what extent cerebellar processing may contribute such a sensorimotor task. Here, we subjected knock-out mice, which suffer impaired intrinsic in their Purkinje cells long-term potentiation at parallel fiber-to-Purkinje cell synapses (L7-PP2B), an task with time response window...
Developmental, cellular, and subcellular variations in the direction of neuronal Cl<sup>–</sup> currents elicited by GABA<sub>A</sub> receptor activation have been frequently reported. We found a corresponding variance reversal potential (E<sub>GABA</sub>) for synapses originating from individual interneurons onto single pyramidal cell. These findings suggest similar heterogeneity cytoplasmic intracellular concentration chloride ([Cl<sup>–</sup>]<sub>i</sub>) dendrites. determined...
Background: There is evidence for dysregulated cholesterol homeostasis in Huntington’s disease (HD). The brain-specific metabolite 24(S)-hydroxycholesterol (24(S)-OHC) decreased manifest HD. 24(S)-OHC an endogenous positive allosteric modulator (PAM) of the N-methyl-D-aspartate (NMDA) receptor, suggesting lower may contribute to NMDA receptor hypofunction We hypothesized changes would be associated with cognitive impairment early Objective: To determine interactions between oxysterols...
In in vitro models of acute brain injury, neuronal death may overwhelm the capacity for microglial phagocytosis, creating a queue dying neurons awaiting clearance. Neurons undergoing programmed cell are this queue, and most visible frequently quantified measure after injury. However, size should be equally sensitive to changes rate phagocytosis. Using rodent organotypic hippocampal slice cultures as model perinatal serial imaging demonstrated that phagocytosis was overwhelmed 2 weeks....
Epileptic networks are characterized by two outputs: brief interictal spikes and rarer, more prolonged seizures. Although either output state is readily modeled in silico induced experimentally, the transition mechanisms unknown, part because no models exhibit both states spontaneously. In small-world neural were built using single-compartment neurons whose physiological parameters derived from dual whole-cell recordings of pyramidal cells organotypic hippocampal slice cultures that...
Abstract Background Polyamine catabolism plays a key role in maintaining intracellular polyamine pools, yet its physiological significance is largely unexplored. Here, we report that the disruption of leads to severe cerebellar damage and ataxia, demonstrating fundamental maintenance function integrity. Methods Mice with simultaneous deletion two principal catabolic enzymes, spermine oxidase spermidine/spermine N 1 -acetyltransferase ( Smox / Sat1 -dKO), were generated by crossbreeding -KO −...
After acute brain injury, neuronal apoptosis may overwhelm the capacity for microglial phagocytosis, creating a queue of dying neurons awaiting clearance. The size this should be equally sensitive to changes in death and rate phagocytosis. Using rodent organotypic hippocampal slice cultures as model perinatal serial imaging demonstrated that phagocytosis was overwhelmed two weeks. Altering rates, e.g. by changing number microglia, dramatically changed visibly neurons. Similar effects were...
Abstract Developmental, cellular, and subcellular variations in the direction of neuronal Cl − currents elicited by GABA A receptor activation have been frequently reported, we found a corresponding variance reversal potential (E ) for individual interneurons synapsing on single pyramidal cell. These findings suggest cytoplasmic concentration ([Cl i ]). We determined [Cl ] by: 1) two-photon imaging sensitive, ratiometric fluorescent protein SuperClomeleon (sCLM); 2) Fluorescence Lifetime...