A5076858404- Cell death mechanisms and regulation
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Carcinogens and Genotoxicity Assessment
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- DNA Repair Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- NF-κB Signaling Pathways
- MicroRNA in disease regulation
- Corneal Surgery and Treatments
- Pluripotent Stem Cells Research
- Autophagy in Disease and Therapy
- Renal Diseases and Glomerulopathies
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Renal and related cancers
- Bone Tissue Engineering Materials
- PARP inhibition in cancer therapy
- Immune Cell Function and Interaction
- Muscle Physiology and Disorders
University of Chieti-Pescara
2015-2024
Temple University
2023-2024
Università Cattolica del Sacro Cuore
2024
Weatherford College
2024
University of Pisa
2014
Uniformed Services University of the Health Sciences
2006
University of Ferrara
2001-2003
Istituto per il Rilevamento Elettromagnetico dell'Ambiente
1996-2003
University of Trieste
2003
University of Helsinki
2003
Tissue engineering is widely recognized as a promising approach for bone repair and reconstruction. Several attempts have been made to achieve materials that must be compatible, osteoconductive, osteointegrative mechanical strength provide structural support. Composite scaffolds consisting in biodegradable natural polymers are very constructs. Hydroxyapatite (HAp) can support alginate inorganic reinforcement osteoconductive component of alginate/HAp composite scaffolds. Therefore,...
Endothelial cells express tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) receptors, but the function of TRAIL in endothelial is not completely understood. We explored role regulation key intracellular signal pathways cells. The addition to primary human increased phosphorylation nitric oxide synthase (eNOS), NOS activity, and NO synthesis. Moreover, induced cell migration cytoskeleton reorganization an NO-dependent manner. did activate NF-κB or COX-2 Instead, prostanoid...
Extracellular vesicles (EVs) are secreted from many tumors, including glioblastoma multiforme (GBM), the most common and lethal brain tumor in adults, which shows high resistance to current therapies poor patient prognosis. Given relevance of information provided by cancer cell secretome, we performed a proteomic analysis microvesicles (MVs) exosomes (EXOs) released GBM-derived stem cells (GSCs). The latter, obtained GBM patients, expressed P2X7 receptors (P2X7Rs), positively correlate with...
The human placenta is an important source of stem cells that can be easily collected without ethical concerns since it usually discarded after childbirth. In this study, we analyzed the amniotic membrane (AM) from with aim mapping different regions respect to their morpho-functional features and regenerative potential. AMs were obtained 24 healthy women, undergoing a caesarean section, mapped into 4 according position in relation umbilical cord: central, intermediate, peripheral, reflected...
Pterygium is a progressive disease of the human eye arising from sub-conjunctival tissue and extending onto cornea. Due to its invasive growth, pterygium can reach pupil compromising visual function. Currently available medical treatments have limited success in suppressing efficiently disease. Previous studies demonstrated that curcumin, polyphenol isolated rhizome
Abstract The aim of this work was to evaluate the involvement survival pathways in response Jurkat T leukaemic cells sensitive cytotoxic action tumour necrosis factor (TNF)‐related apoptosis‐inducing ligand (TRAIL)/Apo2L. express TRAIL‐R2/DR5 and TRAIL‐R4/DcR2 receptors start die by apoptosis early (3 h) upon TRAIL administration reaching a dose‐dependent increase percentage dead within 48 h (up 85–90%). This cell death is accompanied significant ( P < 0.05) G 0 /G 1 phase cycle reverted...
Satellite cell (SC) proliferation and differentiation have critical roles in skeletal muscle recovery after injury adaptation response to hypertrophic stimuli. Normal ageing hinders SC differentiation, is associated with increased expression of a number pro-apoptotic factors muscle. In light previous studies that demonstrated age-related altered genes involved antioxidant repair activity, this investigation was aimed at evaluating the incidence apoptotic features human SCs. Primary cells...
Human amniotic fluid-derived stem cells (AFSCs) represent a novel class of broadly multipotent sharing characteristics both embryonic and adult cells. However, the origin these their actual properties in terms pluripotent differentiation potential are still debated. In order to verify presence features pluripotency human second trimester AFSCs, we have investigated ability form vitro three-dimensional aggregates, known as embryoid bodies (EBs), express specific genes (ESCs) primordial germ...
Mesenchymal Stem Cells derived from Amniotic Fluid (AFMSCs) are multipotent cells of great interest for regenerative medicine. Two predominant cell types, that is, Epithelial-like (E-like) and Fibroblast-like (F-like), have been previously detected in the amniotic fluid (AF). In this study, we examined AF 12 donors observed prevalence E-like phenotype 5, whereas F-like morphology was 7 samples. These phenotypes showed slight differences membrane markers, with higher CD90 lower Sox2 SSEA-4...
The potential clinical applications of human amniotic membrane (hAM) and epithelial cells (hAECs) in the field regenerative medicine have been known literature since long. However, it has yet to be elucidated whether hAM contains different anatomical regions with plasticity differentiation potential. Recently, for first time, we highlighted many differences terms morphology, marker expression, capabilities among four distinct hAM, demonstrating peculiar functional features hAEC populations....
Abstract We recently demonstrated the activation of phosphatidylinositol 3‐kinase (PI3‐K/Akt) survival pathway in Jurkat T leukemia cells known for their sensitivity to tumor necrosis factor (TNF)‐related apoptosis‐inducing ligand (TRAIL)/Apo2L cytotoxic action. The present investigation was done elucidate role cAMP‐response element‐binding (CREB) protein this system. were treated with 100–1,000 ng/ml TRAIL time intervals up 24 h presence or absence selective pharmacologic inhibitors...
Extracellular vesicles (EVs) released from tumor cells are actively investigated, since molecules therein contained and likely transferred to neighboring cells, supplying them with oncogenic information/functions, may represent cancer biomarkers and/or druggable targets. Here, we characterized by a proteomic point of view two EV subtypes isolated sequential centrifugal ultrafiltration technique culture medium glioblastoma (GBM)-derived stem-like (GSCs) obtained surgical specimens human GBM,...