Adrenoceptor subtypes have been localized in human lung by an autoradiographic method, using [125I]iodocyanopindolol (ICYP) to label beta-receptors tissue sections. The ICYP was incubated with cryostat sections of microscopically normal method on microscope slides for 2 h at 37 degrees C. Nonspecific binding determined incubating adjacent serial the presence 200 microM (-)-isoproterenol. Specific binding, which accounted greater than 90% total counts bound sections, saturable, high affinity,...

Brain edema and swelling is a critical factor in the high mortality morbidity associated with traumatic brain injury (TBI). Despite this, mechanisms its development are poorly understood interventions have not changed over 30 years. Although neuropeptides neurogenic inflammation been implicated peripheral formation, their role of central nervous system after trauma has investigated. This study examines neuropeptide, substance P (SP), functional deficits rats. After severe diffuse TBI adult...

To comprehensively review the existing studies of articaine in dentistry and conduct a systematic meta-analysis to answer following Population, Intervention, Comparison Outcome question: "Is safe efficacious local anaesthetic for routine dental treatment compared lidocaine?"Database searches were conducted Medline Ovid, Pubmed, Scopus, Emcare, Proquest Cochrane Central register Controlled Trials. Inclusion criteria all English, human, randomised controlled trials interventions involving 4%...

Magnesium (Mg) declines after traumatic brain injury (TBI), a decline believed associated with ensuing neuronal cell death and subsequent functional impairment. While Mg's effects on motor cognitive deficits following TBI have been well studied, few studies addressed post-traumatic depression as an outcome parameter, despite its being major clinical problem incidence of between 6 77%. We investigated the depression/anxiety in animal model diffuse TBI, explored use magnesium sulfate (MgSO(4))...

The usefulness of administrating adjunctive systemic antibiotics to expedite healing periodontal tissues is a topic interest given the lack clear guidelines.To compare clinical outcomes in patients azithromycin (AZ), amoxicillin plus metronidazole (AMX + MTZ), or scaling and root planing (SRP) alone treatment moderate-to-severe chronic periodontitis.Thirty-eight were randomly assigned into: SRP alone; 500 mg AMX 400 MTZ three times per day for 7 days; AZ 3 days. Antibiotics administered...

The mechanisms associated with edema formation after traumatic brain injury (TBI) have not been fully elucidated. In peripheral tissue injury, the neurogenic component of inflammation plays a significant role in increased vascular permeability and formation. However, few studies examined neuropeptide induced following TBI. Adult male Sprague-Dawley rats were either left untreated, or pre-treated capsaicin (125 mg/kg s.c.) equal volume vehicle, injured 14 days later using 2-meter...

1. Glucocorticoids are an effective treatment in the amelioration of chronic lung disease neonates. However, systemic administration glucocorticoids to neonates is associated with significant side-effects that preclude them as early intervention prevent onset condition. Conversely, local intratracheal may inflammatory insult lungs without development side-effects. We therefore investigated whether delivery corticosteroids could be facilitated using surfactant a vehicle. 2. Addition...

Traumatic brain injuries represent a leading cause of death and disability in the paediatric adult populations. Moderate-to-severe are associated with blood–brain barrier dysfunction, development cerebral oedema, neuroinflammation. Antagonists tachykinin NK1 receptor have been proposed as potential agents for post-injury treatment TBI. We report on identification EUC-001 clinical candidate novel TBI therapy. is selective antagonist high affinity human (Ki 5.75 × 10−10 M). It has sufficient...

Magnesium (Mg) deficiency has been shown to increase substance P release and induce a pro-inflammatory response that can be attenuated with the administration of P-antagonist. Neurogenic inflammation also implicated in traumatic brain injury (TBI), condition where intracellular free magnesium (Mg(f)) decline is known occur correlated functional outcome. We therefore examined whether antagonist restores concentration following TBI.Male, adult Sprague-Dawley rats were injured using Cernak...

A number of test paradigms have been used to determine acute and chronic motor cognitive deficits after experimental traumatic brain injury (TBI). Some involve daily testing either trained or untrained animals whereas others utilize periodic over extended time periods. Which paradigm is the most appropriate for assessment is, however, unclear. In current study, we both weekly in ascertain which protocol suited detection functional diffuse TBI rats. Animals were subjected severe using...

A high-performance liquid chromatography (HPLC) assay is described for the determination of indomethacin in porcine plasma using acetonitrile to precipitate proteins and one-step extraction. Calibration curves (using internal standard method) are linear (r2 > 0.98) over concentration range 50.0 3000 ng/mL both mobile phase plasma. Precision, expressed as inter- intraday coefficient variation (n = 5), < 7% on same day 5% between days at each control sample 300, 1000, ng/mL, respectively....

Regulated uterine contractions are important in many reproductive functions such as sperm transport and embryo positioning during implantation. The role of classical neurotransmitters including acetylcholine norepinephrine regulating myometrial contractility has been well studied; however, the peripheral sensory neurokinins is less clear. major substance P, neurokinin A, B, which predominantly activate receptors (NK-Rs) 1, 2, 3, respectively. This study utilized selective receptor agonists...