A5089007444- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microbial Metabolic Engineering and Bioproduction
- Biochemical Acid Research Studies
- Chemistry and Chemical Engineering
- Folate and B Vitamins Research
- Various Chemistry Research Topics
- Mobile Learning in Education
- Trypanosoma species research and implications
- Natural Antidiabetic Agents Studies
- Coconut Research and Applications
- Mangiferin and Mango Extracts
- Educational Games and Gamification
- Biodiesel Production and Applications
- Microbial bioremediation and biosurfactants
- HIV/AIDS drug development and treatment
- Teaching and Learning Programming
Srinakharinwirot University
2019-2024
Mahidol University
2014-2022
Several of the enzymes related to folate cycle are well-known for their role as clinically validated antimalarial targets. Nevertheless serine hydroxymethyltransferase (SHMT), one key this cycle, efficient inhibitors have not been described so far. On basis plant SHMT from an herbicide optimization program, highly potent Plasmodium falciparum (Pf) and vivax (Pv) with a pyrazolopyran core structure were identified. Cocrystal structures PvSHMT solved at 2.6 Å resolution. These ligands showed...
Trihydroxyphenolic acids such as 3,4,5-trihydroxycinnamic acid (3,4,5-THCA) 4c and 2-(3,4,5-trihydroxyphenyl)acetic (3,4,5-THPA) 2c are strong antioxidants that potentially useful medicinal agents. Our results show p-hydroxyphenylacetate (HPA) 3-hydroxylase (HPAH) from Acinetobacter baumannii can catalyze the syntheses of 3,4,5-THPA 3,4,5-THCA 4-HPA 2a p-coumaric 4a, respectively. The wild-type HPAH convert completely into within 100 min (total turnover number (TTN) 100). However, enzyme...
The oxygenase component (C2) of p-hydroxyphenylacetate (4-HPA) 3-hydroxylase (HPAH) from Acinetobacter baumannii catalyzes the hydroxylation various phenolic acids. In this report, we found that substitution a residue close to group binding site yield S146A variant resulted in an enzyme is more effective than wild-type catalyzing 4-aminophenylacetate (4-APA). Product yields for both and enzymes are better at lower pH values. Multiple turnover reactions indicate first hydroxylate 3-APA give...
The coronavirus (COVID-19) pandemic prevented students from attending on-site classes. Consequently, their face-to-face interactions decreased. Thus, it became crucial to design learning activities enhance the motivation of and inspire them achieve desired outcomes during long disruption Mobile devices applications represent most frequently used alternatives online tools. During COVID-19 pandemic, biochemists faced challenges integrating technology biochemistry principles allow investigate...
Serine hydroxymethyltransferase (SHMT) catalyzes the transfer of a hydroxymethyl group from l-serine to tetrahydrofolate yield glycine and 5,10-methylenetetrahydrofolate. Our previous investigations have shown that SHMTs Plasmodium spp. (P. falciparum, Pf; P. vivax, Pv) are different enzyme rabbit liver in SHMT can use d-serine as substrate. In this report, biochemical biophysical properties human cytosolic form (hcSHMT) enzymes including ligand binding kinetics were investigated. The data...
We have employed computational approaches-FireProt and FRESCO-to predict thermostable variants of the reductase component (C1 ) (4-hydroxyphenyl)acetate 3-hydroxylase. With additional aid experimental results, two C1 variants, A166L A58P, were identified as thermotolerant enzymes, with thermostability improvements 2.6-5.6 °C increased catalytic efficiency 2- to 3.5-fold. After heat treatment at 45 °C, both remain active generate reduced flavin mononucleotide (FMNH- for reactions catalyzed by...
This research developed learning activities for students to explore and connect the concepts of acid–base titration, equivalence points, pKa, molar absorption coefficients. Polyphenolic compounds consisting p-coumaric acid (CMA), caffeic (CFA), 3,4,5-trihydroxycinnamic (3,4,5-THCA) were selected as study models. The inquiry-based consisted four phases, including in-classroom, prelaboratory discussion, in-laboratory work, postlaboratory discussion. was conducted through a single group...