A5004512582- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Malaria Research and Control
- Bacteriophages and microbial interactions
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Trypanosoma species research and implications
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Molecular Communication and Nanonetworks
- Bacillus and Francisella bacterial research
- thermodynamics and calorimetric analyses
- Biotin and Related Studies
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Toxin Mechanisms and Immunotoxins
- Probiotics and Fermented Foods
- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Biomedical Research and Pathophysiology
- Amino Acid Enzymes and Metabolism
National Center for Genetic Engineering and Biotechnology
2012-2025
National Science and Technology Development Agency
2012-2025
Imperial College London
2018-2019
Whole-cell biosensors can form the basis of affordable, easy-to-use diagnostic tests that be readily deployed for point-of-care (POC) testing, but to date detection analytes such as proteins cannot easily diffuse across cell membrane has been challenging. Here we developed a novel biosensing platform based on agglutination using an E. coli whole-cell biosensor surface-displaying nanobodies which bind selectively target protein analyte. As proof-of-concept, show feasibility this design detect...
Several of the enzymes related to folate cycle are well-known for their role as clinically validated antimalarial targets. Nevertheless serine hydroxymethyltransferase (SHMT), one key this cycle, efficient inhibitors have not been described so far. On basis plant SHMT from an herbicide optimization program, highly potent Plasmodium falciparum (Pf) and vivax (Pv) with a pyrazolopyran core structure were identified. Cocrystal structures PvSHMT solved at 2.6 Å resolution. These ligands showed...
There is a growing need for low-cost, portable technologies the detection of threats to environment and human health. Here we propose label-free, optical whole-cell Escherichia coli biosensor 3-phenoxybenzoic acid (3-PBA), biomarker monitoring exposure synthetic pyrethroid insecticides. The functions like competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as element. When engineered cells are mixed with 3-PBA-protein conjugate crosslinking that can be visually...
Abstract Nanobodies (Nbs) hold great potential to replace conventional antibodies in various biomedical applications. However, methods for their discovery can be time-consuming and expensive. We have developed a reliable protein selection strategy that combines magnetic activated cell sorting (MACS)-based screening of yeast surface display (YSD) libraries functional ligand-binding identification by Tat-based recognition associating proteins (FLI-TRAP) isolate antigen-specific Nbs from...
Dihydropteroate synthase (DHPS) is a known sulfa drug target in malaria treatment, existing as bifunctional enzyme together with hydroxymethyldihydropterin pyrophosphokinase (HPPK). Polymorphisms key residues of Plasmodium falciparum DHPS (PfDHPS) have been characterized and linked to resistance malaria. Genetic sequencing P. vivax dhps (Pvdhps) from clinical isolates has shown several polymorphisms at the positions equivalent those Pfdhps genes conferring resistance, suggesting mechanism...
Plasmodial bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) is a validated antimalarial drug target. In this study, expression of the putative dhfr-ts Plasmodium ovale rescued DHFR chemical knockout and TS null bacterial strain, demonstrating its catalytic functions. PoDHFR-TS was expressed in Escherichia coli BL21 (DE3) affinity purified by Methotrexate Sepharose column. Biochemical enzyme kinetics characterizations indicated that similar to other plasmodial enzymes,...
Plasmodium parasites, the causative agent of malaria, rely heavily on de novo folate biosynthesis, and enzymes in this pathway have therefore been explored extensively for antimalarial development. Serine hydroxymethyltransferase (SHMT) from spp., an enzyme involved recycling dTMP synthesis, has shown to catalyze conversion L- D-serine glycine (Gly) a THF-dependent reaction, mechanism which is not yet fully understood. Here, crystal structures P. vivax SHMT (PvSHMT) binary complex with...
This opinion piece describes a new design for the remediation of recalcitrant biofilms. It builds on previous work to develop engineered E. coli that recognize quorum sensing signals from pathogens in biofilm and secrete toxins response. To solve challenge dilute signalling molecules, we propose use nanobodies enzymes displayed surface cells localize them degrade extracellular polymeric substances, thus creating solution with better ‘seek destroy’ capabilities.
Bacterial ghosts (BGs) are nonviable empty bacterial cell envelopes with intact cellular morphology and native surface structure. BGs made from pathogenic bacteria used for biomedical pharmaceutical applications. However, incomplete inactivation during BG preparation raises safety concerns that could limit the intended use. Therefore, safer types needed production. Here, we produced food-grade Gram-positive bacterium Lactobacillus plantarum TBRC 2-4 by conditional expression of a...
ABSTRACT Affordable, easy-to-use diagnostic tests that can be readily deployed for point-of-care (POC) testing are key in addressing challenges the diagnosis of medical conditions and improving global health general. Ideally, POC should highly selective biomarker, user-friendly, have a flexible design architecture low cost production. Here we developed novel agglutination assay based on whole E. coli cells surface-displaying nanobodies which bind selectively to target protein analyte. As...