A5038427170- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Advanced ceramic materials synthesis
- Click Chemistry and Applications
- Fungal Biology and Applications
- Chemical Synthesis and Reactions
- Metallurgy and Material Forming
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Aluminum Alloys Composites Properties
- Immune Response and Inflammation
- Metal and Thin Film Mechanics
- Synthetic Organic Chemistry Methods
- Organophosphorus compounds synthesis
- Advanced materials and composites
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Synthesis and biological activity
- Oxidative Organic Chemistry Reactions
- Synthesis and Characterization of Heterocyclic Compounds
- Synthesis and Catalytic Reactions
- Fungal Plant Pathogen Control
- Boron and Carbon Nanomaterials Research
- Plant biochemistry and biosynthesis
- S100 Proteins and Annexins
- Cancer therapeutics and mechanisms
RIKEN
2003-2024
RIKEN Center for Sustainable Resource Science
2017
RIKEN Advanced Science Institute
2006-2016
Wako University
2007-2016
European Society of Anaesthesiology
2007
Tokyo University of Science
1987-2006
Harvard University
2006
RIKEN Center for Brain Science
2001
Antibiotic Research UK
2001
Higashi Osaka City General Hospital
1996-2000
Abstract Polyketides form many clinically valuable compounds. However, manipulation of their biosynthesis remains highly challenging. An understanding gene cluster evolution provides a rationale for reprogramming the biosynthetic machinery. Herein, we report characterization giant modular polyketide synthases (PKSs) responsible production aminopolyol polyketides. Heterologous expression over 150 kbp clusters successfully afforded products, whose stereochemistry was established by taking...
AbstractThe high-temperature mechanical behaviour of copper, Cu–Al alloys, and nickel has been examined using torsional testing with hollow testpieces in conjunction microstructural observations on deformed quenched specimens both optical electron microscopy. Dynamic recrystallization occurred these materials as the restoration process during deformation. The factors influencing dynamic have considered, including high stacking fault energy. It was found that regime transition flow stress...
Genome sequencing of Streptomyces species has highlighted numerous potential genes secondary metabolite biosynthesis. The mining cryptic is important for exploring chemical diversity. Here we report the metabolite-guided genome and functional characterization a gene by biochemical studies. Based on systematic purification metabolites from sp. SN-593, isolated novel compound, 6-dimethylallylindole (DMAI)-3-carbaldehyde. Although many 6-DMAI compounds have been variety organisms, an enzyme...
Abstract This paper presents a new technique, termed femtosecond laser shock peening ablation in liquids (fs-LSPAL), which can realize simultaneous crack micro/nanomanufacturing and hierarchical micro/nanolaser ablation, giving rise to the formation of diverse multiscale structures, such as macroporous ratcheted structures en échelon microfringes decorated with parabolic nanoripples. Through analysis surface morphologies, many phenomena have been confirmed take place during fs-LSPAL,...
A potent inhibitor of a dual‐specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during screening microbial metabolites. This identified as 4‐isoavenaciolide (4‐iA), and determined to irreversibly inhibit phosphatase activity with 50% inhibitory concentration 1.2 μM. Detailed tandem mass spectrometry analyses proteolysed fragments revealed that two molecules 4‐iA bound molecule at the different fragments: one containing catalytic domain other α6 helix positioned surface...
We previously reported that the blood-brain barrier (BBB) function was deteriorated in vessels located along hippocampal fissures stroke-prone spontaneously hypertensive rats (SHRSP). In this study, we examined changes of gene expression BBB-damaged SHRSP.Vascular samples were microdissected from hippocampi SHRSP and Wistar-Kyoto (WKY) as a control difference between without BBB damage WKY by microarray. The differences protein brain tissues two strains using real-time quantitative reverse...
Growth factors were immobilized with photo-reactive gelatin and used for serum-free human mesenchymal stem cell (hMSC) culturing.
We previously reported that the blood-brain barrier (BBB) function was impaired in vessels hippocampus 3-month-old stroke-prone spontaneously hypertensive rats (SHRSP). In this study, we examined gene and protein expressions of P-glycoprotein, a representative efflux transporter cerebral vessels, BBB-damaged hippocampal SHRSP Wistar Kyoto (WKY) as controls, to clarify roles vessels.The expression P-glycoprotein cortical samples by real-time quantitative reverse transcriptase-polymerase chain...
We have constructed a natural products database, "RIKEN Natural Products Encyclopedia (RIKEN NPEdia)", as an informatics part of chemical resource bank, "the RIKEN Depository NPDepo)". Approximately 25,000 compound data are now listed in NPEdia, some which were transferred from "KNApSAcK", secondary metabolites database by Prof. Kanaya and his colleagues (http://kanaya.aist-nara.ac.jp/KNApSAcK/, http://prime.psc.riken.jp/ (mirror site)). Compounds NPEdia mainly isolated actinomycetes, fungi,...