A5101458161- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Synthetic Organic Chemistry Methods
- Marine Biology and Environmental Chemistry
- Natural product bioactivities and synthesis
- Plant Toxicity and Pharmacological Properties
- Sesquiterpenes and Asteraceae Studies
- Chemical synthesis and alkaloids
- Fungal Biology and Applications
- Phytochemical compounds biological activities
- Chemical Synthesis and Analysis
- Synthesis and Biological Activity
- Marine Ecology and Invasive Species
- Protein Structure and Dynamics
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Plant biochemistry and biosynthesis
- Enzyme Structure and Function
- Analytical Chemistry and Chromatography
- Traditional and Medicinal Uses of Annonaceae
- Mass Spectrometry Techniques and Applications
- Steroid Chemistry and Biochemistry
- Cancer Treatment and Pharmacology
- Asymmetric Synthesis and Catalysis
Nihon University
2016-2025
Japan Atomic Energy Agency
2024
RIKEN
2006-2021
RIKEN Center for Integrative Medical Sciences
2005-2017
RIKEN Center for Sustainable Resource Science
2015-2017
RIKEN Advanced Science Institute
2009-2014
Nippon Soken (Japan)
2014
Yokohama City University
2002-2012
Center for Genomic Science
2003-2012
Yokohama National University
2012
Mussel power: The structures and configurations have been determined of four new indole alkaloids, notoamides A–D, which were isolated from mussel-derived Aspergillus sp. Notoamides A–C show moderate cytotoxicity against cancer cell lines. relationship between these other metabolites suggests that the fungus uses a diverse biosynthetic pathway. Marine-derived fungi proven to be rich sources structurally novel biologically active secondary metabolites, are emerging as significant resource for...
Structure-based virtual screening is carried out using molecular docking programs. A number of such programs are currently available, and the selection program difficult without knowing characteristics or performance each program. In this study, performances three programs, DOCK, AutoDock, GOLD, were evaluated with 116 target proteins. The validated two novel standards, along a traditional enrichment rate measurement. For evaluations, run was repeated 1000 times initial conformations ligand....
Zinc (Zn) is an essential trace metal required by many enzymes and transcription factors for their activity or the maintenance of structure. Zn has a variety effects in immune responses inflammation, although it not been well known how affects these reactions on molecular basis. We here showed that suppresses Th17-mediated autoimmune diseases at lest part inhibiting development Th17 cells via attenuating STAT3 activation. In mice injected with type II collagen to induce arthritis, treatment...
Many benthic marine invertebrates, like barnacles, have a planktonic larval stage whose primary purpose is dispersal. How these species colonize suitable substrata fundamental to understanding their evolution, population biology, and wider community dynamics. Unlike dispersal, settlement occurs on relatively small spatial scale involves behavior in response physical chemical characteristics of the substratum. Biogenic cues been implicated this process. Their identification, however, has...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBioactive marine metabolites. Part 35. Cytotoxic metabolites of the sponge Mycale adhaerens LambeNobuhiro Fusetani, Takeo Sugawara, Shigeki Matsunaga, and Hiroshi HirotaCite this: J. Org. Chem. 1991, 56, 16, 4971–4974Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August 1991https://pubs.acs.org/doi/10.1021/jo00016a031https://doi.org/10.1021/jo00016a031research-articleACS PublicationsRequest...
Six new prenylated indole alkaloids, named notoamides F−K (8−13), were isolated from a marine-derived Aspergillus sp. Their structures, including absolute configurations, elucidated by spectroscopic methods. Notoamide I (11) showed weak cytotoxicity against HeLa cells with an IC50 value of 21 μg/mL.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGambieric acids: unprecedented potent antifungal substances isolated from cultures of a marine dinoflagellate Gambierdiscus toxicusHiroshi Nagai, Koichiro Torigoe, Masayuki Satake, Michio Murata, Takeshi Yasumoto, and Hiroshi HirotaCite this: J. Am. Chem. Soc. 1992, 114, 3, 1102–1103Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January...
Antipodal (−)-versicolamide B and notoamides L−N were isolated from a marine-derived Aspergillus sp. The possible biosynthetic pathway of enantiomeric pairs notoamide versicolamide are proposed. Notoamide L is the first metabolite containing 25 carbons in related prenylated indole alkaloids. M potentially precursor to proposed azadiene species involved putative intramolecular Diels−Alder reaction biogenesis bicyclo[2.2.2]diazaoctane ring system.
Notoamides O−R were isolated from a marine-derived Aspergillus sp. Notoamide O possesses novel hemiacetal/hemiaminal ether functionality hitherto unknown among this family of prenylated indole alkaloids. The structure represents an unusual branch point for the oxidative modification other members in alkaloids biogenetic pathway.
Polytheonamide B (pTB), a highly cytotoxic polypeptide, is one of the most unusual nonribosomal peptides sponge origin. pTB linear 48-residue peptide with alternating d- and l-amino acids contains total eight types nonproteinogenic amino acids. To investigate mechanisms underlying its activity, we determined three-dimensional structure by NMR spectroscopy, calculation, energy minimization. adopts single right-handed β6.3-helical in 1:1 mixture methanol/chloroform length approximately 45 Å...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrbiculamide A: a novel cytotoxic cyclic peptide from marine sponge Theonella spNobuhiro Fusetani, Takeo Sugawara, Shigeki Matsunaga, and Hiroshi HirotaCite this: J. Am. Chem. Soc. 1991, 113, 20, 7811–7812Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September 1991https://pubs.acs.org/doi/10.1021/ja00020a080https://doi.org/10.1021/ja00020a080research-articleACS PublicationsRequest reuse...
A new oroidin dimer, mauritiamine (1), has been isolated from the marine sponge Agelas mauritiana along with known (2) and 4,5-dibromopyrrole-2-carbamide (3). Their structures were determined by spectral analysis. Compounds 1 2 inhibited larval metamorphosis of barnacle Balanus amphitrite ED50 values 15 19 μg/mL, respectively, while 3 promoted ascidian Ciona savignyi at a concentration 2.5 μg/mL.
Notoamide E was identified to be a short-lived precursor in the biosynthesis of prenylated indole alkaloids mussel-derived Aspergillus sp. In addition, feeding experiment (13)C-labeled notoamide afforded structurally novel metabolites.
Human CA125, encoded by the MUC16 gene, is an ovarian cancer antigen widely used for a serum assay. Its extracellular region consists of tandem repeats SEA domains. In this study we determined three-dimensional structure domain from murine homologue using multidimensional NMR spectroscopy. The forms unique alpha/beta sandwich fold composed two alpha helices and four antiparallel beta strands has characteristic turn named TY-turn between alpha1 alpha2. internal mobility main chain low...