A5074250337- Pharmacogenetics and Drug Metabolism
- Plant tissue culture and regeneration
- Analytical Chemistry and Chromatography
- Computational Drug Discovery Methods
- Pesticide and Herbicide Environmental Studies
- Microbial Metabolic Engineering and Bioproduction
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant biochemistry and biosynthesis
- Weed Control and Herbicide Applications
- Insect Resistance and Genetics
- Microfluidic and Capillary Electrophoresis Applications
- Steroid Chemistry and Biochemistry
- Bacterial Genetics and Biotechnology
- Plant Gene Expression Analysis
- Allelopathy and phytotoxic interactions
- RNA and protein synthesis mechanisms
- Eicosanoids and Hypertension Pharmacology
- Electron Spin Resonance Studies
- Enzyme Production and Characterization
- Genomics, phytochemicals, and oxidative stress
- Drug Transport and Resistance Mechanisms
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
Kobe University
2012-2023
Toyama Prefectural University
2011
Hyogo Prefectural Institute of Technology
2011
Showa Pharmaceutical University
2011
Genomics (United Kingdom)
2002-2009
Yamaguchi University
1992-1994
Kyoto University
1989-1991
Kyoto Institute of Technology
1986-1990
Coplanar polychlorinated biphenyls included in dioxin-like compounds are bio-accumulated and adversely affect wildlife human health. Although many researchers have studied the metabolism of PCBs, there been few reports vitro 3,3',4,4',5-pentachlorobiphenyl (PCB126), despite fact that it has highest toxicity among PCB congeners. Cytochrome P450 (CYP) 1A1 proteins can metabolize some dioxins PCBs by hydroxylation, but activities rat CYP1A1 very different. The mechanism remains unclear. From...
Cytochrome P450 (P450 or CYP) monooxygenases play an important role in the oxidation of a number lipophilic substrates including secondary metabolites higher plants. Larkin reported that CYP78A1 was preferentially expressed developing inflorescences Zea mays (Larkin, Plant Mol. Biol. 25: 343-353, 1994). However, enzymatic function hasn't been clarified yet. To characterized activity CYP78A1, this study, cDNA and tobacco yeast NADPH-cytochrome oxidoreductase reductase) Saccharomyces...
We report an efficient and reproducible method to generate a microarray of model biological membranes on solid substrate by applying the inkjet printing technology. Although is currently widely used for industrial fabrication processes, including materials, lipid remains technically challenging due hydrophobic nature droplets instability bilayer structure against dehydration. In present study, we printed lipids onto glass covered with micropatterned membrane polymeric phospholipid bilayer....
Aminoxyl radical formation from DMPO in the presence of Fe ion was studied to clarify ambiguous ESR signals obtained by spin trapping with DMPO. It found that when used a Fenton system, Fe-DMPO complex formed immediately. This subsequently attacked oxidative species originating H2O2 and thus degradation induced system. On other hand, case M, PO, be very slow, indicating 3 position favorably species. Some products are probably aminoxyl radicals. series reactions may compete make it difficult...
The traditional Japanese alcoholic beverage sake is produced by fermentation of rice Saccharomyces cerevisiae and Aspergillus oryzae. A. oryzae releases ferulic acid, an antioxidant, from steamed during the process. concentration acid increased with time production rate peaked 9–12 days post inoculation. Analysis cultures oryzae, high-performance liquid chromatography (HPLC), revealed that p-coumaric induced 18.9-fold increase in level acid. Furthermore, SDS-PAGE analysis or decrease...
In order to overcome a common problem in spin trapping with high concentrations of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) where ESR spectra are obtained which include an unidentified set lines composed triplet doublets, commercial DMPO was analyzed for its impurities by high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy. It has been determined that this undesirable spectrum is due impurity included the trap. This compound assigned...
CYP 2A6 is a human enzyme that metabolizes many xenobiotics including coumarin, indole, nicotine and carcinogenic nitrosamines. The gene for CYP2A6 polymorphic. There are few data available to clarify the relationship between P450 genetic variants metabolism of materials in food. wild-type protein 13 mutants (CYP2A6.1, CYP2A6.2, CYP2A6.5, CYP2A6.6, CYP2A6.7, CYP2A6.8, CYP2A6.11, CYP2A6.15, CYP2A6.16, CYP2A6.17, CYP2A6.18, CYP2A6.21, CYP2A6.23 CYP2A6.25) were co-expressed with...
We describe a stable and functional model biological membrane based on polymerized lipid bilayer with chemically modified surface. A was formed from mixture of two diacetylene-containing phospholipids, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (DiynePE). DiynePC structure, whereas the ethanolamine headgroup DiynePE enabled molecules to be grafted onto Copolymerization resulted in robust bilayer....
A number of studies have demonstrated that cytochrome P450 (P450) converts furanocoumarin derivatives into reactive molecules, which form covalent bonds to biomolecules. 5-Methoxypsoralen (5-MOP) is a natural from apiaceous plants. In this study, we examined the effect on 5-MOP metabolism single nucleotide polymorphisms (SNPs) in CYP2A13. We used <i>Escherichia coli</i>-generated recombinant enzymes wild-type CYP2A13*1 and five variants, CYP2A13*4 (R101Q), CYP2A13*5 (F453Y), CYP2A13*6...
An assaying method of cytochrome P450 (P450 or CYP) monooxygenase activities for toxicological evaluation drugs and environmental pollutants was developed by immobilizing on an oxygen sensoring layer. Membrane fractions from E. coli expressing human were entrapped in agarose silica-based gels immobilized 96-well microarrays having sensing film at the bottom. The made organically modified silica (ORMOSIL) doped with Tris(4,7-diphenyl-1,10-phenanthroline) ruthenium dichloride...