A5063733171- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Visual perception and processing mechanisms
- Neural dynamics and brain function
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Glaucoma and retinal disorders
- Biochemical Analysis and Sensing Techniques
- Axon Guidance and Neuronal Signaling
- Olfactory and Sensory Function Studies
- Retinal Diseases and Treatments
- Memory and Neural Mechanisms
- Animal Behavior and Reproduction
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Optical Imaging and Spectroscopy Techniques
- Nerve injury and regeneration
- Optical Coherence Tomography Applications
- Anesthesia and Neurotoxicity Research
- Hearing, Cochlea, Tinnitus, Genetics
- Vestibular and auditory disorders
- Biochemical effects in animals
- CCD and CMOS Imaging Sensors
- Functional Brain Connectivity Studies
University of Virginia
2001-2025
Northwestern University
2009-2018
Institute of Neurobiology
2009-2017
Brandeis University
2016
University of California, San Francisco
2004-2010
W. M. Keck Foundation
2004-2008
Foundation Center
2005
University of California, San Diego
2003
U.S. National Science Foundation
2000-2002
One of the first steps in coding olfactory information is transformation synaptic input to action potential firing mitral and tufted (M/T) cells mammalian bulb. However, little known regarding mechanisms underlying this process vivo. In study, we examined odor-evoked response patterns M/T granule using whole-cell recording anesthetized, freely breathing rats. We find that excitatory responses typically consist bursts potentials coupled approximately 2 Hz respiration rhythm. Odor-evoked,...
The mouse is a promising model in the study of visual system function and development because available genetic tools. However, quantitative analysis receptive field properties had not been performed superior colliculus (SC) despite its importance vision usefulness developmental studies. We have made single-unit extracellular recordings from superficial layers SC urethane-anesthetized C57BL/6 mice. first map fields with flashing spot stimuli show that most neurons spatially overlapped ON OFF...
The dorsal lateral geniculate nucleus (dLGN) receives visual information from the retina and transmits it to cortex. In this study, we made extracellular recordings in dLGN of both anesthetized awake mice, found that a surprisingly high proportion cells were selective for stimulus orientation. orientation selectivity was unchanged after silencing cortex pharmacologically, indicating is not due cortical feedback. tuning some correlated with their elongated receptive fields, while others...
Significance Rett syndrome is a neurodevelopmental disorder caused by mutations in methyl-CpG-binding protein 2 ( MeCP2 ). It thought to result from altered neuronal connectivity and/or plasticity, possibly through abnormal experience-dependent synapse development, but the underlying mechanisms remain obscure. Using -null mice, we examined development of neural circuits primary visual cortex where GABAergic interneurons regulate critical period plasticity. We provide evidence that precocious...
Purpose.: Glaucoma is characterized by retinal ganglion cell (RGC) death and frequently associated with elevated IOP. How RGCs degenerate before little understood, so we sought to investigate RGC degeneration in a mouse model of ocular hypertension. Methods.: A laser-induced chronic hypertension mimicked human high-tension glaucoma. Immunohistochemistry was used characterize overall loss an optomotor behavioral test measure corresponding changes visual capacity. Changes functional properties...
The superior colliculus (SC) is a layered midbrain structure important for multimodal integration and sensorimotor transformation. Its superficial layers are purely visual receive depth-specific projections from distinct subtypes of retinal ganglion cells. Here we use two-photon calcium imaging to characterize the response properties neurons in most lamina mouse SC, an undersampled population with electrophysiology. We find that these have compact receptive fields primarily overlapping ON...
The superficial superior colliculus (sSC) carries out diverse roles in visual processing and behaviors, but how these functions are delegated among collicular neurons remains unclear. Here, using single-cell transcriptomics, we identified 28 neuron subtypes subtype-enriched marker genes from tens of thousands adult mouse sSC neurons. We then asked whether the sSC's molecular tuned to different stimuli. Specifically, imaged calcium dynamics single vivo during stimulation mapped gene...
The responses of cells in the visual cortex to stimulation two eyes changes dramatically following a period monocular deprivation (MD) during critical early life. This phenomenon, referred as ocular dominance (OD) plasticity, is widespread model for understanding cortical plasticity. In this study, we designed stimulus patterns and quantification methods analyze OD mouse using optical imaging intrinsic signals. Using periodically drifting bars restricted binocular portion field, obtained...
The orderly projections from retina to superior colliculus (SC) preserve a continuous retinotopic representation of the visual world. development retinocollicular maps depend on combination molecular guidance cues and patterned neural activity. Here, we characterize functional in mice lacking molecules ephrin-A2, -A3, -A5 deficient both ephrin-As structured spontaneous retinal activity, using method Fourier imaging intrinsic signals. We find that SC ephrin-A2/A3/A5 triple knock-out contains...
Purpose.: We investigated the progressive degeneration of retinal and superior collicular functions in a mouse model sustained ocular hypertension. Methods.: Focal laser illumination injection polystyrene microbeads were used to induce chronic Retinal ganglion cell (RGC) loss was characterized by vivo optical coherence tomography (OCT) immunohistochemistry. dysfunction also monitored full-field ERG. light responses recorded using 256-channel multielectrode array (MEA), RGC subtypes...
We seek to develop techniques for high-resolution imaging of the tree shrew retina visualizing and parameterizing retinal ganglion cell (RGC) axon bundles in vivo. applied visible-light optical coherence tomography fibergraphy (vis-OCTF) temporal speckle averaging (TSA) visualize individual RGC retina. For first time, we quantified bundle width, height, cross-sectional area vis-OCT angiography (vis-OCTA) microvasculature shrews. Throughout retina, as distance from optic nerve head (ONH)...
Topographic maps are a fundamental feature of the brain's representations sensory environment as well an efficient way to organize motor control networks. Although great progress has been made in our understanding map development, very little is known about how topographic for develop and interface with maps. Here we representation eye movements superior colliculus (SC) awake mice. As stimulation sites were sampled along anterior–posterior axis, small amplitude, nasally directed...