A5005592166- Cellular Mechanics and Interactions
- Skin Protection and Aging
- Cell Adhesion Molecules Research
- Textile materials and evaluations
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- thermodynamics and calorimetric analyses
- Skin and Cellular Biology Research
- Marine Invertebrate Physiology and Ecology
- Thermoregulation and physiological responses
- Plant Surface Properties and Treatments
- Invertebrate Immune Response Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Medicinal Plants and Neuroprotection
- Advancements in Transdermal Drug Delivery
- Microtubule and mitosis dynamics
- Immune Response and Inflammation
- Polymer Surface Interaction Studies
- Cancer Cells and Metastasis
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Animal testing and alternatives
- Pharmacological Effects of Natural Compounds
bioMérieux (France)
2020-2024
Centre National de la Recherche Scientifique
2017-2024
Centre de Recherche en Cancérologie de Lyon
2019-2024
Université Claude Bernard Lyon 1
2017-2024
Inserm
2017-2024
École Normale Supérieure de Lyon
2017-2021
Laboratoire de Biologie et Modélisation de la Cellule
2017-2020
The regulation of morphogenesis by the basement membrane (BM) may rely on changes in its mechanical properties. To test this, we developed an AFM-based method to measure BM stiffness during two key processes Drosophila ovarian follicle development. First, elongation depends epithelial cells that collectively migrate secreting fibrils perpendicularly anteroposterior axis. Our data show increases this migration and fibril incorporation enhances stiffness. In addition, heterogeneity, due...
Anas barbariae 200K, a homeopathic medicine, is traditionally used for influenza-like illnesses. We investigated the effects of 200K on microglial cells, subpopulation macrophages specific to central nervous system often study inflammatory processes and oxidative stress generated during episodes. The demonstrates effect cell stiffness reactive oxygen species production using atomic force microscopy fluorescence techniques, respectively. Our results showed that rapidly increased in resting...
Skin homeostasis and self-renewal are partially maintained by interfollicular stem cells (ISCs), located in the basal layer above dermal papillae of dermo-epidermal junction (DEJ). Aging leads to a decline skin renewal concurrent reduction cell potential. It is also marked disorganization extracellular matrix both DEJ dermis, flattening DEJ. To better understand ISC aging, new methods needed characterize ISCs their environment. Since mechanical properties substrate influence fate, we...
It is unknown how growth in one tissue impacts morphogenesis a neighboring tissue. To address this, we used the Drosophila ovarian follicle, which cluster of 15 nurse cells and posteriorly located oocyte are surrounded by layer epithelial cells. known that as grow, overlying flatten wave begins anterior. Here, demonstrate an anterior to posterior gradient decreasing cytoplasmic pressure present across this acts through TGFβ control both triggering progression cell flattening. Our data...
Because plant cells are glued to each other via their cell walls, failure coordinate growth among adjacent can create cracks in tissues. Here, we find that the unbalanced of inner and outer tissues clavata3 de-etiolated3 (clv3 det3) mutant Arabidopsis thaliana stretched epidermal cells, ultimately generating stems. Stem slowed before appeared along clv3 det3 stems, whereas pith became drastically distorted accelerated growth, yielding stress, after appearance cracks. This is consistent with...
Human skin is particularly vulnerable to age-related deterioration and undergoes profound structural functional changes, reflected in the external appearance. Skin ageing characterized by features such as wrinkling or loss of elasticity. Even if research advances have been done concerning molecular mechanisms that underlie these very few studies conducted structure stiffness organ a whole. In this study, we showed, thanks human reconstructs Japanese Medaka fish model, biomechanics new...
The intrinsic biomechanical properties of cancer cells remain poorly understood. To decipher whether cell stiffness modulation could increase melanoma cells’ invasive capacity, we performed both in vitro and vivo experiments exploring by atomic force microscopy (AFM). We correlated with morphology adaptation the molecular mechanisms underlying epithelial-to-mesenchymal (EMT)-like phenotype switching. found that reduction was systematically associated acquisition cutaneous lines, human skin...
Abstract It is unknown how growth in one tissue impacts morphogenesis a neighboring tissue. To address this, we used the Drosophila ovarian follicle, where cluster of 15 nurse cells and posteriorly located oocyte are surrounded by layer epithelial cells. known that as grow, overlying flatten wave begins anterior. Here, demonstrate an anterior to posterior gradient decreasing cytoplasmic pressure present across this acts through TGFß control both triggering progression cell flattening. Our...
Background: The level of oxidative stress is a known marker inflammation. Microglial cells are macrophages present in the central nervous system and play important roles inflammatory responses making microglial powerful model for studying Homeopathic medicines often used to alleviate inflammation clinical practice Oscillococcinum® indicated treatment flu-like symptoms. However, date, mechanism action unknown. anti-oxidative effect homeopathic dilutions Arnica montana, Arsenicum album,...
Abstract Human skin is submitted to various factors leading either extrinsic or intrinsic aging. In addition inducing molecular and morphological changes, aging characterized by modifications in biomechanical properties modifications. These can be used as biomarkers both evaluate the degree of advancement but also detect potential pathologies. Hence, it from critical interest able fully monitor these alterations. Here we introduce a portable innovative ® device, EASYSTIFF , which thought...